Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Metabolic Flux Analysis of Beijerinckia indica for PS-7 Production

  • Wu Jian-Rong (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Son Jeong Hwa (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Seo Hyo-Jin (Interdisciplinary Program of Marine Biotechnology, Pukyong National University) ;
  • Kim Ki-Hong (Aquatic Life of Medicine, Pukyong National University) ;
  • Nam Yoon-Kwon (Aquaculture, Pukyong National University) ;
  • Lee Jin-Woo (Division of Biotechnology, Dong-A University) ;
  • Kim Sung-Koo (Department of Biotechnology and Bioengineering, Pukyong National University)
  • Published : 2005.02.01
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Abstract

In order to investigate central metabolic changes in Beijerinckia indica, cells were grown on different carbon sources and intracellular flux distributions were studied under varying concentrations of nitrogen. Metabolic fluxes were estimated by combining material balances with extracellular substrate uptake rate, biomass formation rate, and exopolysaccharide (EPS) accumulation rate. Thirty-one metabolic reactions and 30 intracellular metabolites were considered for the flux analysis. The results revealed that most of the carbon source was directed into the Entner-Doudoroff pathway, followed by the recycling of triose-3-phosphate back to Hexose­6-phosphate. The pentose phosphate pathway was operated at a minimal level to supply the precursors for biomass formation. The different metabolic behaviors under varying nitrogen concentrations were observed with flux analysis.

Keywords

References

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