Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Genetic characteristics of the Korean isolate KI -1 of Toxoplasma gondii

  • LIN Aifen (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • SHIN Eun-Hee (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • KIM Tae-Yun (Institute of Malariology and Department of Parasitology, Inje University College of Medicine) ;
  • PARK Jae-Hwan (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • GUK Sang-Mee (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • CHAI Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center)
  • Published : 2005.03.01
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Abstract

Toxoplasma gondii tachyzoites were isolated from an ocular patient in the Republic of Korea and maintained in the laboratory (designated KI-1). In the present study, its genotype was determined by analyzing dense granule antigen 6 (GRA6) gene and surface antigen 2 (SAG2) gene as typing markers. Digestion of the amplification products of GRA6 and of the 5' and 3' ends of SAG2, respectively, with Mse I, Sau3A I, and Hha I, revealed that KI-1 is included in the genotype I, which includes the worldwide virulent RH strain. In addition, when the whole sequences of the coding regions of SAG1, rhoptry antigen 1 (ROP1), and GRA8 genes of KI-1 were compared with those of RH, minor nucleotide polymorphisms and amino acid substitutions were identified. These results show that KI-1 is a new geographical strain of T. gondii that can be included in the genotype I.

Keywords

References

  1. Chai JY, Lin A, Shin EH, Oh MD, Han ET, Nan HW, Lee SH (2003) Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea. Korean J Parasitol 41: 147-154 https://doi.org/10.3347/kjp.2003.41.3.147
  2. Dubey JP, Graham DH, Blackston CR, Lehmann T, Gennari SM, Ragozo AM, Nishi SM, Shen SK, Kwok OC, Hill DE, Thulliez P (2002) Biological and genetic characterization of Toxoplasma gondii isolates from chickens (Gallus domesticus) from Sao Paulo, Brazil: unexpected findings. Int J Parasitol 32: 99-105 https://doi.org/10.1016/S0020-7519(01)00364-2
  3. Fazaeli A, Carter PE, Darde ML, Pennington TH (2000a) Molecular typing of Toxoplasma gondii strains by GRA6 gene sequence analysis. Int J Parasitol 30: 637-642 https://doi.org/10.1016/S0020-7519(00)00036-9
  4. Fazaeli A, Carter PE, Pennington TH (2000b) Intergenic spacer (IGS) polymorphism: a new genetic marker for differentiation of Toxoplasma gondii strains and Neospora caninum. J Parasitol 86: 716-723 https://doi.org/10.1645/0022-3395(2000)086[0716:ISIPAN]2.0.CO;2
  5. Grigg ME, Bonnefoy S, Hehl AB, Suzuki Y, Boothroyd JC (2001a). Success and virulence in Toxoplasma as the result of sexual recombination between two distinct ancestries. Science 294: 161-165 https://doi.org/10.1126/science.1061888
  6. Grigg ME, Ganatra J, Boothroyd JC, Margolis TP (2001b) Unusual abundance of atypical strains associated with human ocular toxoplasmosis. J Infect Dis 184: 633-639 https://doi.org/10.1086/322800
  7. Hogdall E, Vuust J, Lind P, Petersen E (2000) Characterization of Toxoplasma gondii isolates using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) of the non-coding Toxoplasma gondii (TGR)-gene sequences. Int J Parasitol 30: 853-858 https://doi.org/10.1016/S0020-7519(00)00059-X
  8. Howe DK, Honore S, Derouin F, Sibley LD (1997) Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxoplasmosis. J Clin Microbiol 35: 1411-1414
  9. Howe DK, Sibley LD (1995) Toxoplasma gondii comprises three clonal lineages: Correlation of parasite genotype with human disease. J Infect Dis 172: 1561-1566 https://doi.org/10.1093/infdis/172.6.1561
  10. Johnson AM (1998) Is there more than one species in the genus Toxoplasma? Tokai J Exp Clin Med 23: 383-389
  11. Lehmann T, Blackston CR, Parmley SF, Remington JS, Dubey JP (2000) Strain typing of Toxoplasma gondii: comparison of antigen-coding and housekeeping genes. J Parasitol 86: 960-971 https://doi.org/10.1645/0022-3395(2000)086[0960:STOTGC]2.0.CO;2
  12. Lyons RE, Johnson AM (1998) Gene sequence and transcription differences in 70 kDa heat shock protein correlate with murine virulence of Toxoplasma gondii. Int J Parasitol 28: 1041-1051 https://doi.org/10.1016/S0020-7519(98)00074-5
  13. Sibley LD, Boothroyd JC (1992) Virulent strains of Toxoplasma gondii comprise a single clonal lineage. Nature 359: 82-85 https://doi.org/10.1038/359082a0
  14. Suzuki Y, Conley FK, Remington JS (1989) Differences in virulence and development of encephalitis during chronic infection vary with the strain of Toxoplasma gondii. J Infect Dis 159: 790-794 https://doi.org/10.1093/infdis/159.4.790