Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
권호 표지

Characteristics and Improving Breed of Economic Traits of Korea Native Chicken

한국 재래 닭 품종 특성 및 초기성장 개량을 위한 분자표지 개발

  • Oh J. D. (Genomic Informatics Center, Hankyong National University) ;
  • Park M. H. (Genomic Informatics Center, Hankyong National University) ;
  • Kong H. S. (Animal Products Grading Service) ;
  • Lee H. K. (Genomic Informatics Center, Hankyong National University) ;
  • Jeon G. J. (Genomic Informatics Center, Hankyong National University) ;
  • Yeon S. H. (National Livestock Research Institute) ;
  • Sang B. D. (National Livestock Research Institute) ;
  • Choi C. H. (National Livestock Research Institute) ;
  • Cho B. W. (Department of Animal Science, Miryang National University)
  • Published : 2005.03.01
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Abstract

This study was conducted to estimate the effects of genotype for chicken major histocompatibility complex (MHC) B-LB genes on economic traits. To detect polymorphism, 400 bp fragments of MHC B-LB genes were obtained and sequenced. After digestions using restriction enzyme Hea III, two restriction enzyme sites were observed. There were two mutations at position 427 and 651 those were decided as Type I and Type II, respectively. Using RFLP analyses, type I were genotyped to TT, TC and CC, and type II to MM, Mm and mm. The relatively higher TC genotype frequencies (0.8) of Type I and Mm genotype frequencies (0.88) of Type II were observed in Korean native chickens. The effects of the genotype on 150 days body weight trait were investigated by the associations of CC and Mm genotypes (P<0.05) in Korean native chickens. This result suggests that a significant association exists between the SNP and 150 days body weight.

본 연구는 한국 재래 닭의 유전적 특성을 분자표지를 이용하여 그 차이를 규명하고 초기성장에 미치는 영향을 분석하여 이를 이용한 재래닭의 개량을 목적으로 실시하였다. MHC class II B-LB 유전자 내의 염기변이체가 경제형질에 미치는 영향에 대하여 연구하였다. MHC class II B-LB유전자 내 400 bp 크기의 유전자를 증폭하여 염기서열 분석과 제한효소 처리를 이용한 다형성 분석을 실시하였다. 연구결과 두 개의 제한효소 절단지역이 발견되었으며 427 지역을 Type I 으로 651 지역은 Type II로 정하여 RFLP 분석을 실시하였다. Type I지역의 유전자형은 TT, TC, CC로 나타났으며, TypeII 지역의 유전자형은 MM, Mm, mm으로 나타났다. TC와 Mm 유전자형이 다른 유전자형과 비교하였을 때 한국재래 닭에서 높은 출현빈도를 보였다(0.8, 0.88). 유전자형이 한국 재래 닭의 150일령 체중에 미치는 영향을 분석한 결과 CC와 Mm 유전자형에서 통계적 유의성이 도출되었다 (P<0.05). 따라서 본 연구의 결과를 이용하여 한국 재래 닭의 유전적 특성을 규명할 수 있으며 초기 성장이 높은 성적을 나타내는 CC, Mm 유전자형을 개량에 이용하게 된다면 큰 효과를 얻을 수 있을 것으로 사료되어진다. 본 연구의 결과는 차후 한국 재래 닭의 과학적이고 지속적인 유전자원의 보존과 육종 전략에 있어 매우 유용하게 활용될 것으로 기대된다.

Keywords

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