Archives of Pharmacal Research

  • 제28권2호
  • /
  • Pages.238-242
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  • 2005
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  • 0253-6269(pISSN)
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  • 1976-3786(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Benzodiazepine System is Involved in Hyperalgesia in Rats Induced by the Exposure to Extremely Low Frequency Magnetic Fields

  • Jeong Ji Hoon (Department of Pharmacology, College of Pharmacy, Chung Ang University) ;
  • Choi Kyung Bum (Department of Pharmacology, College of Pharmacy, Chung Ang University) ;
  • Moon Nam Ju (Department of Ophthalmology, Chung Ang University) ;
  • Park Eon Sub (Department of Pathology, College of Medicine, Chung Ang University) ;
  • Sohn Uy Dong (Department of Pharmacology, College of Pharmacy, Chung Ang University)
  • 발행 : 2005.02.01
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초록

Many reports demonstrate that extremely low frequency magnetic fields (ELF MFs, 60 Hz) may be involved in hyperalgesia. In a previous investigation, we suggested that MFs may produce hyperalgesia and such a response may be regulated by the benzodiazepine system. In order to further confirm this effect of MFs, we used diazepam and/or flumazenil with MFs exposure. When testing the pain threshold of rats using hot plate tests, MFs or diazepam ($0.5\;{\mu}g$, i.c.v.; a benzodiazepine receptor agonist) induced hyperalgesic effects with the reduction of latency. These effects were blocked by a pretreatment of flumazenil (1.5 mg/kg, i.p.; a benzodiazepine receptor antagonist). When the rats were exposed simultaneously to MFs and diazepam, the latency tended to decrease without statistical significance. The induction of hyperalgesia by co-exposure to MFs and diazepam was also blocked by flumazenil. However, the pretreatment of GABA receptor antagonists such as bicuculline ($0.1\;{\mu}g$, i.c.v.; a $GABA_A$ antagonist) or phaclofen ($10\;{\mu}g$, i.c.v.; a $GABA_B$ antagonist) did not antagonize the hyperalgesic effect of MFs. These results suggest that the benzodiazepine system may be involved in MFs-induced hyperalgesia.

키워드

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