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The Pharmaceutical Society of Korea (대한약학회)
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In Vitro Inhibitory Effect of Triterpenoidal Saponins from Platycodi Radix on Pancreatic Lipase

  • Xu Bao Jun (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, The Pharmaceutical Institute, Dalian University) ;
  • Han Li Kun (Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto) ;
  • Zheng Yi Nan (College of Chinese Material Medicine, Jilin Agricultural University) ;
  • Lee Jeong Hyun (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University) ;
  • Sung Chang Keun (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University)
  • Published : 2005.02.01
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Abstract

In the process of investigating anti-obesity effect of Platycodi Radix, we found that aqueous extract of Platycodi Radix might inhibit intestinal absorption of dietary fat by inhibiting pancreatic lipase (PL) activity. In order to clarify the anti-obesity mechanism of Platycodi Radix, activity-guided isolation was performed to find active components. The total saponin fraction of Platycodi Radix appeared to have a potent inhibitory activity against the hydrolysis of triolein emulsified with phosphatidycholine by pancreatic lipase in vitro. Based on these results, further purification of active components yielded 10 known triterpenoidal saponins, among these compounds, platycodin A, C, D, and deapioplatycodin D exhibited significant inhibitory effects on PL at the concentration of $500\;{\mu}g/mL$ with 3.3, 5.2, 34.8, and $11.67\%$ pancreatic lipase activity vs control, respectively. Platycodin D was found to inhibit the PL activity in a dose-dependent manner. Therefore, the anti-obesity effect of Platycodi Radix might be due to the inhibition of pancreatic lipase by its saponins.

Keywords

References

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