YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Protective Effect of Yangguksanwha-tang Metabolized by Liver Homogenate on Hypoxia-reperfusion Induced PC12 Cell Damage

간효소에 의해 대사된 양격산화탕의 저산소/재관류로부터 PC12 세포 보호효과

  • Soh Yunjo (Department of Neuroscience, Graduate School of East-West Medical Science, Kyung Hee University)
  • 소윤조 (경희대학교 동서의학대학원 신경과학교실)
  • Published : 2005.02.01
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Abstract

The protective effect of Yangguksanwha-tang (YST) against hypoxia-reperfusion insult was investigated in PC12 cells. To elucidate the mechanism of the protective effect of YST, cell viability, the changes in activities of superoxide dismutase, glutathione peroxidase, catalase, caspase 3 and the production of malondialdehyde were observed after treating PC12 cells with YST which was metabolized by rat liver homogenate. Pretreatment of YST with liver homogenate appeared to increase its protective effect against hypoxia-reperfusion insult. The result showed that YST had the highest protective effect against hypoxia/reperfusion at the dose of $2\;{\mu}g/ml$ in PC12 cells, probably by recovering the redox enzyme activities and MDA to control level.

Keywords

References

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