Tuberculosis and Respiratory Diseases

  • 제59권5호
  • /
  • Pages.517-521
  • /
  • 2005
  • /
  • 1738-3536(pISSN)
  • /
  • 2005-6184(eISSN)
대한결핵및호흡기학회 (The Korean Academy of Tuberculosis and Respiratory Diseases)
권호 표지

삼출성 흉수에서 기질 금속단백분해효소-1과 금속단백분해효소의 조직억제제-1

Matrix Metalloproteinase-1 and Tissue Inhibitor of Metalloproteinase-1 levels in Exudative Pleural Effusions

  • 조재화 (인하대학교 의과대학 내과학교실) ;
  • 남정현 (인하대학교 의과대학 진단검사의학교실) ;
  • 이금호 (인하대학교 의과대학 내과학교실) ;
  • 윤병갑 (인하대학교 의과대학 내과학교실) ;
  • 류정선 (인하대학교 의과대학 내과학교실) ;
  • 곽승민 (인하대학교 의과대학 내과학교실) ;
  • 이홍렬 (인하대학교 의과대학 내과학교실)
  • Cho, Jae Hwa (Department of Internal Medicine, College of Medicine, Inha University) ;
  • Nam, Jeong Hyeon (Department of Clinical Pathology, College of Medicine, Inha University) ;
  • Lee, Kyum Ho (Department of Internal Medicine, College of Medicine, Inha University) ;
  • Yoon, Byeong Kab (Department of Internal Medicine, College of Medicine, Inha University) ;
  • Ryu, Jeong Sun (Department of Internal Medicine, College of Medicine, Inha University) ;
  • Kwak, Sung Min (Department of Internal Medicine, College of Medicine, Inha University) ;
  • Lee, Hong Lyeol (Department of Internal Medicine, College of Medicine, Inha University)
  • 투고 : 2005.05.02
  • 심사 : 2005.09.21
  • 발행 : 2005.11.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

배 경 : 흉수에서 기질 금속단백분해효소(MMP)와 금속단백분해효소 조직억제제(TIMP)는 감염과 면역질환의 병인에 중요한 역할을 한다. 저자들은 삼출성 흉수에서 MMP-1과 TIMP-1의 발현에 대한 측정을 하였다. 방 법 : 결핵성 흉막염 33예, 악성흉수 17예, 심부전 또는 간경화 흉수환자 5예의 흉막천자액에서 세포분석, 생화학적 분석을 하였고, 효소면역측정방법으로 MMP-1과 TIMP-1(Biotrack$^{TM}$, Amersharm Pharmacia Biotech, Freiburg, FRG)를 측정하였다. 결 과 : 흉수 MMP-1은 결핵성 흉수(n=33, $12.1{\pm}8.8ng/mL$)가 악성 흉수(n=17, $4.8{\pm}4.0ng/mL$)나 누출액(n=5, $2.6{\pm}1.5ng/mL$)보다 높았다(p=0.002). 흉수 TIMP-1은 결핵성 흉수(n=23, $110.9{\pm}38.2{\mu}g/mL$)가 누출성 흉수(n=5, $53.7{\pm}21.8{\mu}g/mL$)보다 높았고(p=0.004), 악성 흉수(n=17, $90.2{\pm}39.8{\mu}g/mL$)도 누출성 흉수보다 높았으나(p=0.039), 결핵성 흉수와 악성 흉수는 유의한 차이가 없었다(p=0.132). 결 론 : 흉수 MMP-1, TIMP-1는 결핵성 흉막염에서 심부전이나 간경화 흉수보다 높았다.

Background : The balances of the proteinases and antiproteinases system have been implicated in the pathogenesis of various exudative pleural effusions. The aim of this study was to examine the matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) levels in exudative pleural effusions. Methods : The study included 33 tuberculous effusions, 17 malignant, and 5 transudates. The pleural levels of MMP-1 and TIMP-1 were determined using a commercially available ELISA assay. Results : The group of tuberculous effusions showed higher pleural MMP-1 levels than the malignant and transudates. The pleural TIMP-1 levels of the tuberculous and malignant effusions were higher than the transudates. Conclusion : Elevated pleural MMP-1 and TIMP-1 levels were found in tuberculous effusions.

키워드

참고문헌

  1. Antony VB, Godbey SW, Kunkel SL, Hott JW, Hartman DL, Burdick MD, et al. Recruitment of inflammatory cells to the pleural space: chemotactic cytokines, IL-8 and monocyte chemotactic peptide-1 in human pleural fluids. J Immunol 1993;151:7216-23
  2. Philip-Joet F, Alessi MC, Philip-Joet C, Alilaud M, Barriere JR, Arnaud A, et al. Fibrolytic and inflammatory processes in pleural effusion. Eur Respir J 1995;8:1352-6 https://doi.org/10.1183/09031936.95.08081352
  3. Hurewitz AN, Zucker S, Mancusco P, Wu CL, Dimassimo B, Lysik RM, et al. Human pleural effusions are rich in matrix metalloproteases. Chest 1992;102: 1808-14 https://doi.org/10.1378/chest.102.6.1808
  4. Birkedal-Hansen H, Moore WG, Bodden MK, Windsor LJ, Birkedal-Hansen B, DeCarlo A, et al. Matrix metalloproteinase: a review. Crit Rev Oral Biol Med 1993; 4:197-250 https://doi.org/10.1177/10454411930040020401
  5. Stetler-Stevenson WG. Dynamics of matrix turnover during pathologic remodeling of the extracellular matrix. Am J Pathol 1996;148:1345-50
  6. O'Connor CM, Fitzgerald MX. Matrix metalloproteases and lung disease. Thorax 1994;49:602-9 https://doi.org/10.1136/thx.49.6.602
  7. Eickelberg O, Sommerfeld CO, Wyser C, Tamm M, Reichenberger F, Bardin PG, et al. MMP and TIMP expression in pleural effusions of different origin. Am J Respir Crit Care Med 1997;156:1987-92 https://doi.org/10.1164/ajrccm.156.6.9704112
  8. Light RW, MacGregor MI, Luchsinger PC, Ball WC Jr. Pleural effusion: the diagnostic separation of transudates and exudates. Ann Intern Med 1972;77:507-13 https://doi.org/10.7326/0003-4819-77-4-507
  9. Ocana I, Martinez-Vazquenz JM, Segura RM, Fernandez-DeSevilla T, Capdevila JA. Adenosine deaminase in pleural fluid: test for diagnosis of tuberculous pleural effusion. Chest 1983;84:51-3 https://doi.org/10.1378/chest.84.1.51
  10. Murphy G, Doherty AJ. The matrix metalloproteinase and their inhibitors. Am J Respir Cell Mol Biol 1992; 7:120-5 https://doi.org/10.1165/ajrcmb/7.2.120
  11. Segura-Valdez L, Pardo A, Gaxiola M, Uhal BD, Becerril C, Selman M. Upregulation of gelatinases A and B, collagenase 1 and 2, and increased parenchymal cell death in COPD. Chest 2000;117:684-94 https://doi.org/10.1378/chest.117.3.684
  12. Hoheisel G, Sack U, Hui DS, Huse K, Chan KS, Chan KK, et al. Occurance of matrix metalloproteinases and tissue inhibitors of metalloproteinases in tuberculous pleuritis. Tuberculosis 2001;81:203-9 https://doi.org/10.1054/tube.2000.0276
  13. Chang JC, Wysocki A, Tchou-Wong KM, Moskowitz N, Zhang Y, Rom WN. Effect of Mycobacterium tuberculosis and its components on macrophages and the release of matrix metalloproteinases. Thorax 1996;51: 306-11 https://doi.org/10.1136/thx.51.3.306
  14. Iglesias D, Alegre J, Aleman C, Ruiz E, Soriano T, Armadans LI, et al. Metalloproteinases and tissue inhibitors of metalloproteinases in exudative pleural effusions. Eur Respir J 2005;25:104-9 https://doi.org/10.1183/09031936.04.00010504
  15. Odeh M, Sabo E, Srugo I, Oliven A. Correlation between polymorphonuclear leukocyte counts and levels of tumor necrosis factor- in pleural $\alpha$ fluid of patients with parapneumonic effusion. Lung 2002;180:265-71 https://doi.org/10.1007/s004080000100