Journal of Industrial Technology (산업기술연구)
- Volume 25 Issue B
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- Pages.241-251
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- 2005
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- 1229-9588(pISSN)
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- 1598-1371(eISSN)
Antitumor Activity and Effect on Cell Proliferation and Differenciation of Exopolysaccharide Produced by Submerged Cultivation of Ganoderma lucidum
영지(Ganoderma lucidum)의 액체배양에 의한 세포외 다당의 항암활성과 세포증식 및 분화에 미치는 영향
- Published : 2005.08.31
Abstract
Exopolysaccharide (CBP) from submerged culture broth of Ganoderma lucidum mycelium and the water soluble (BWS) and water insoluble (BWI) fractions of CBP were prepared by gel filtration. Antitumor activity and effects on proliferation and differenciation of human cancer cells and mouse NIH 3T3 cells were studied. Cytotoxicity test of CBP, BWS and BWI fractions on human cancer cell lines was performed by using sulforhodamine B (SRB) assay. A549 (lung carcinoma), Colo320 DM and HSR (colon carcinoma), and NIH 3T3 cells were used. BWI fraction showed the strongest cytotoxicity (maximum 20% survival) to all human cells tested. However it did not induced apoptosis. Interestingly BWI fraction did not exert cytotoxic effect on NIH 3T3 cells at low concentration of cells (
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