Journal of Preventive Medicine and Public Health

  • 제38권4호
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  • Pages.437-442
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  • 2005
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  • 1975-8375(pISSN)
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  • 2233-4521(eISSN)
대한예방의학회 (The Korean Society for Preventive Medicine)
권호 표지

임산부의 혈중 엽산과 호모시스틴 수준이 태반세포의 DNA 메틸화에 미치는 영향

Folate and Homocysteine Levels during Pregnancy affect DNA Methylation in Human Placenta

  • 박보현 (이화여자대학교 의과대학 예방의학교실) ;
  • 김영주 (이화여자대학교 의과대학 산부인과학교실, 이화여자대학교 의과학연구소) ;
  • 이화영 (이화여자대학교 의과대학 해부학교실, 이화여자대학교 의과학연구소) ;
  • 하은희 (이화여자대학교 의과대학 예방의학교실, 이화여자대학교 의과학연구소) ;
  • 민정원 (이화여자대학교 의과대학 예방의학교실) ;
  • 박종순 (이화여자대학교 의과대학 산부인과학교실) ;
  • 박혜숙 (이화여자대학교 의과대학 예방의학교실, 이화여자대학교 의과학연구소)
  • Park, Bo-Hyun (Departments of Preventive Medicine, Ewha Womans University) ;
  • Kim, Young-Ju (Departments of Obstetrics and Gynecology, Medical Research Institute, Ewha Womans University) ;
  • Lee, Hwa-Young (Departments of Anatomy, Medical Research Institute, Ewha Womans University) ;
  • Ha, Eun-Hee (Departments of Preventive Medicine, Medical Research Institute, Ewha Womans University) ;
  • Min, Jung-Won (Departments of Preventive Medicine, Ewha Womans University) ;
  • Park, Jong-Soon (Departments of Obstetrics and Gynecology, Ewha Womans University) ;
  • Park, Hye-Sook (Departments of Preventive Medicine, Medical Research Institute, Ewha Womans University)
  • 발행 : 2005.12.01
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초록

Objectives : DNA methylation is one of the best characterized epigenetic mechanisms that play a regulatory role in genome programming and imprinting during embryogenesis. In this present study, we investigated the association between DNA methylation in the human placenta and the maternal folate and homocysteine concentrations on the Methylenetetrahydrofolatereductase (MTHFR) genetic polymorphism during pregnancy. Methods : We investigated 107 pregnant women who visited Ewha Woman's University Hospital for prenatal care during their $24{\sim}28$ weeks-period of gestation. During the second trimester, we measured the serum homocysteine and folate concentrations . The MTHFR 677 genetic polymorphism was determine by performing PCR-RFLP assay. The expression of DNA methylation in the human placentas was estimated by using immunohistochemistry method. Results : Serum folate was negatively correlated with the serum homocysteine concentration for all the MTHFR genotypes. We found positive correlation between the folate concentrations and the DNA methylation in the human placenta (p<0.05). An increasing concentration of homocysteine was associated with reduced DNA methylation in the human placenta. The coefficient value was -2.03 (-3.77, -0.29) on the regression model (p<0.05). Conclusion : These findings suggest that the maternal folate and homocysteine levels along with the MTHFR 677 genetic polymorphism during pregnancy affect the DNA methylation in the human placenta.

키워드

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