Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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A Study of Carry Over Contamination in Chematology

이월오염에 대한 연구

  • Chang, Sang-Wu (Technical Information & Quality Assurance Div, Green Cross Reference Laboratories) ;
  • Kim, Nam-Yong (Technical Information & Quality Assurance Div, Green Cross Reference Laboratories) ;
  • Lyu, Jae-Gi (Healthcare Center, Sam Sung Medical Center) ;
  • Jung, Dong-Jin (Department of Laboratory Medicine, Gaemyong University) ;
  • Kim, Gi-You (Department of Laboratory Medicine, St. Vencent's Hospital) ;
  • Park, Yong-Won (Clinical Chematology Dept., Green Cross Reference Laboratories) ;
  • Chu, Kyung-Bok (Clinical Chematology Dept., Green Cross Reference Laboratories)
  • 장상우 (녹십자의료재단 고객지원 및 QA부) ;
  • 김남용 (녹십자의료재단 고객지원 및 QA부) ;
  • 류재기 (삼성서울병원 건강의학센타) ;
  • 정동진 (계명대학교 동산병원 진단검사의학과) ;
  • 김기유 (성빈센트병원 진단검사의학과) ;
  • 박용원 (녹십자의료재단 화학.혈액검사실) ;
  • 추경복 (녹십자의료재단 화학.혈액검사실)
  • Published : 2005.12.30
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Abstract

Carry over contamination has been reduced in some systems by flushing the internal and external surfaces of the sample probe with copious amount of diluent. It between specimens should be kept as small as possible. A built-in, continuous-flow wash reservoir, which allows the simultaneous washing of the interior and exterior of the syringe needles, addresses this issue. In addition, residual contamination can further be prevented through the use of efficient needle rinsing procedures. In discrete systems with disposable reaction vessels and measuring cuvets, any carry over is entirely caused by the pipetting system. In analyzers with reuseable cuvets or flow cells, carry over may arise at every point through which high samples pass sequentially. Therefore, disposable sample probe tips can eliminate both the contamination of one sample by another inside the probe and the carry over of in specimen into the specimen in the cup. The results of the applicative carry over experiment studied on 21 items for total protein (TP), albumin (ALB), total bilirubin (TB), alkaline phosphatase (ALP), aspratate aminotranferase (AST), alanine aminotranferase (ALT), gamma glutamyl transferase (GGT), creatinine kinase (CK), lactic dehydrogenase (LD), creatnine (CRE), blood urea nitrogen (BUN), uric acid (UA), total cholesterol (TC), triglyceride (TG), glucose (GLU), amylase (AMY), calcium (CA), inorganic phosphorus (IP), sodium (Na), potassium (K), chloride (CL) tests in chematology were as follows. Evaluation of process performance less than 1% in all tests was very good, but a percentage of ALB, TP, TB, ALP, CRE, UA, TC, GLU, AMY, IP, K, Na, and CL was 0%, implying no carry over. Other tests were ALT(-0.08%), GGT(-0.09%), CK(0.08%), LD(0.06%), BUN(0.12%), TG (-0.06%), and CA(0.89%).

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