Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Osteoblastic differentiation of adult stem cells by Biphasic Calcium Phosphate

이형상 칼슘 인산염(Biphasic Calcium Phosphate)에 의한 성체 줄기세포의 골아세포 분화

  • Lee, Myoung-Ku (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Lim, Sung-Woo (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Pi, Sung-Hee (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Kim, Yun-Sang (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Shin, Hyung-Shik (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You, Hyoung-Keun (Department of Periodontology, School of Dentistry, Wonkwang University)
  • 이명구 (원광대학교 치과대학 치주과학 교실) ;
  • 임성우 (원광대학교 치과대학 치주과학 교실) ;
  • 피성희 (원광대학교 치과대학 치주과학 교실) ;
  • 김윤상 (원광대학교 치과대학 치주과학 교실) ;
  • 신형식 (원광대학교 치과대학 치주과학 교실) ;
  • 유형근 (원광대학교 치과대학 치주과학 교실)
  • Published : 2005.12.31
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Abstract

The present study was to determine the influence of micro-macro biphasic calcium phosphate(MBCP) on proliferation and differentiation of human marrow-derived mesenchymal stem cells. Primary stem cells were cultured from bone marrow and 3-4 passaged cells were used. This study tested the proliferative effects by cell counting. Collagen sythensis, alkaline phosphatase activity, expression of osteocalcin and bone sialoprotein by Western blot analysis were evaluated. The cellular proliferation of ASC was not influenced by MBCP. Collagen synthesis of ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The ALP activity in ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The expression of OC and BSP incresaed in ASC cultured on MBCP. These results suggest that MBCP may stimulates the osteoblastic activity of ASC.

Keywords

References

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