Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Nitric oxide production and inducible nitric oxide synthase expression induced by Porphyromonas gingivalis lipopolysaccharide

Porphyromonas gingivalis의 세균내독소가 RAW264.7세포에서의 nitric oxide의 생성과 inducible nitric oxide synthase의 발현에 미치는 영향 및 기전

  • Paek, Eun-Young (Department of Periodontology, College of Dentistry, Pusan National University) ;
  • Choi, Eun-Young (Department of Life Science, College of Natural Science, Silla University) ;
  • Choi, Jeom-II (Department of Periodontology, College of Dentistry, Pusan National University) ;
  • Lee, Ju-Yun (Department of Periodontology, College of Dentistry, Pusan National University) ;
  • Kim, Sung-Jo (Department of Periodontology, College of Dentistry, Pusan National University)
  • 백은영 (부산대학교 치과대학 치주과학교실) ;
  • 최은영 (신라대학교 자연대학 생물과학과) ;
  • 최점일 (부산대학교 치과대학 치주과학교실) ;
  • 이주연 (부산대학교 치과대학 치주과학교실) ;
  • 김성조 (부산대학교 치과대학 치주과학교실)
  • Published : 2005.12.31
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Abstract

본 연구는 치주질환 주요 병인균주 중의 하나인 Porphyromonas gingivalis의 세균내독소가 마우스 대식 세포주인 RAW264.7 세포에서의 nitric oxide의 생성과 iNOS의 발현에 미치는 영향을 분석하고 그 기전을 규명하기 위해 수행되었다. Butanol추출법과 phenol-water법에 의해 P. gingivalis 381로부터 세균내독소를 추출하였으며, NO의 생성은 배양 상층액 내의 nitrite 농도를 측정하여 결정하였다. 또한, iNOS의 western blot 분석과 reverse transcription (RT)-PCR 산물의 분석을 수행하였다. P. gingivalis의 세균내독소는 부가적인 자극이 없는 상태에서도 iNOS의 발현과 NO 생성을 유발하였으며, NF- ${\kappa}B$, microtubule polymerization, protein tyrosine kinase, 그리고 protein kinase C 등이 P. gingivalis 세균내독소에 의한 NO 생성에 간여하는 것으로 여겨진다. 또한, P. gingivalis 세균내독소에 의한 NO 생성에는 L-arginine이 요구되었다. P. gingivalis 세균내독소에 의한 NO 생성은 염증성 치주질환의 발병과 진행에 있어 중요한 역할을 하는 것으로 여겨진다.

Keywords

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