Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Prevalence of fimA Genotypes of Porphyromonas gingivalis Strains in peri-implant sulcus

임플란트 주위 치주낭내의 Porphyromonas gingivalis 섬모유전형의 출현율

  • 서동건 (경희대학교 치과대학 치주과학교실) ;
  • 권영혁 (경희대학교 치과대학 치주과학교실) ;
  • 박준봉 (경희대학교 치과대학 치주과학교실) ;
  • 허익 (경희대학교 치과대학 치주과학교실) ;
  • 정종혁 (경희대학교 치과대학 치주과학교실)
  • Published : 2005.12.31
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Abstract

Porphyromonas gingivalis is a gram negative. black-pigmented anaerobe, associated with periodontitis & peri-implantitis. Fimbriae(fimA) of P. gingivalis are filamentous components on the cell surface and important in the colonization and invasion of periodontal tissue. But all P. gnigivalis strains don't have equal pathogenicity, inequality among strains originates from different fimA genotype. P. gnigivalis fimA gene encoding fimbrillin(structural subunit of fimbriae) has been classified into 5 genotypes(types I to V) based on the nucleotide sequences. In the present study, we examined the prevalence of these fimA genotypes in patients with dental implant and the relationship between prevalence of these genotypes and a condition of peri-implant tissue. Dental plaque specimens obtained from 189 peri-implant sulci of 97 patients with dental implants were analyzed by 16S rRNA fimA gene-directed PCR assay. P. gingivalis were detected in 86.2% of the alll samples. Among the P. gingivalis-positive samples, a significant difference in the occurrence of typeII was observed between test and the two control groups. In two control groups, typeII fimA were detected in 6.3%(PD<5mm/BOP-). 18.7%(PD<5mm/BOP+). In the test $group(PD{\geqq}5mm/BOP+)$, type II fimA genotype were detected most frequently in 50.0% . And a correlation between specific fimA types and peri-implantitis was found in $typeII(R^2=l.105)$. These results suggest that P. gingivalis strains that possess typeII fimA are gradually increased, as a condition of peri-implant tissue is getting complicated and are closely associated with peri-implant health status. We speculate that these organisms be involved in peri-implantitis

Keywords

References

  1. Karoussis IK, Salvi GE. Heitz-Mayfield LJA Brägger D, Hämmerle CHF, Lang PL. Long-term implant prognosis in patients with and without a history of chronic periodontitis: a 10-year prospective cohort study of the ITI$^{\circledR}$ Dental Implant system. Clin Oral Impl Res 2003; 14: 329-339 https://doi.org/10.1034/j.1600-0501.000.00934.x
  2. Jemt T, Leckholm U, Adell R. Osseointegrated implants in the treatment of partially edentulous patients: a preliminary study on 876 consecutively placed fixtures, Int J Oral Maxillofac Implants 1989:4:211-217
  3. Rutar A, Lang N, Buser D, Bürgin W, Mombelii A. Retrospective assessment of clinical and microbiological facteors affecting periimplant tissue conditions. Clin Oral Impl Res 2001: 12: 189-195. https://doi.org/10.1034/j.1600-0501.2001.012003189.x
  4. Salcetti JM. Moriarty JD, Cooper LF, Smith FW, Collins JG. The clinical. microbial. and host response charicteristics of the failing implant. Int J Oral Maxillofac Implants 1997:12:32-42
  5. Karoussis IK. Brägger U, Salvi GE, Bürgin W, Lang PL. Effect of implant design on survival and success rates of titanium oral implants: a 10-year prospective cohort study of the ITI Dental Implant system. Clin Oral Impl Res 2004: 15 : 8-17 https://doi.org/10.1111/j.1600-0501.2004.00983.x
  6. Esposito M. Hirsch JM. Lekholm U, Thomsen P. Biological factors contributing to failures of osseointegrated oral implants. (II) Etiopathogenesis. European Journal of Oral Sciences 1998: 106:721-764 https://doi.org/10.1046/j.0909-8836..t01-6-.x
  7. Lee KH, Tanner ACR, Maiden MFJ, Weber HP. Pre- and post-implantation microbiota of the tongue, teeth, and newly-placed implant. J Clin Periodontol 1999: 26: 822-832 https://doi.org/10.1111/j.1600-051X.1999.tb02527.x
  8. Quirynen M. de Soete M, van Steenberghe D. Infectious risks for oral implants: a review of the literature. Clin Oral Impl Res 2002:13:1-19 https://doi.org/10.1034/j.1600-0501.2002.130101.x
  9. Albrektsson T & Isidor F. Consensus report of session IV. In: Lang NP & Karring T, eds. Processing of the 1st Eropean Workshop on Periodontology, 365-369. London: Quintessence Publishing Co., Ltd
  10. Paster BJ, Boches SK, Galvin JL. Ericson RE, Lau CN. Levanos VA. Sahasrabudhe A. Dewhirst FE. Bacterial diversity in human subgingival plaque. J Bacteriol 2001: 183: 3770-3783 https://doi.org/10.1128/JB.183.12.3770-3783.2001
  11. Zambon JJ. Periodontal diseases: microbial factors. Ann Periodontol 1996: 1: 879-925 https://doi.org/10.1902/annals.1996.1.1.879
  12. Sumida S. Ishihara K, Kishi M, Okuda K. Transmission of periodontal disease-associated bacteria from teeth to osseointegrated implant regions. Int J Oral Maxillofac Implants 2002; 17:696-702
  13. Leonhardt A. Adolfsson B. Lekholm U. Wickstrom M. Dahlen G. A longitudinal microbiolgical study on osseointegrated titanium implants in partially edentulous patients. Clin Oral Impl Res 1993 :4: 113-120. https://doi.org/10.1034/j.1600-0501.1993.040301.x
  14. Mombelli A. Van Oosten MAC. Schurch E, Lang NP. The microbiota associated with successful or failing osseointegrated titanium implants. Oral Microbiol and Immunology 1987;2:145-151 https://doi.org/10.1111/j.1399-302X.1987.tb00298.x
  15. Mombelli A. Marxer M. Gaberthuel T. Grunder U. Lang NP. The microbiota of osseointegrated implants in patients with a history of periodontal disease. J Clin Periodontol 1995; 22: 124-130 https://doi.org/10.1111/j.1600-051X.1995.tb00123.x
  16. Heydenrijk K, Meijer HJ. van der Reijden WA. Raghoebar GM. Vissink A. Stegenga B. Microbiota around root-form endosseous implants: a review of the literature. Int J Oral Maxillofac Implants 2002 ; 17: 829-838
  17. Listgarten MA. Lai CH. Comparative microbiological characteristics of failing implants and periodontally diseased teeth. J Periodontol 1999:70:431-437 https://doi.org/10.1902/jop.1999.70.4.431
  18. Becker W. Becker BE. Newman MG, Nyman S. Clinical and microbiologic findings that may contribute to dental implant failure. Int J Oral maxillofac Surg 1990:5:31-38
  19. Van Winkelhoff AJ. Wolf JWA. Actinobacillus actinomycetemcomitans-associated periimplantitis in an edentulous patients: A case report. J Clin periodontol 2000: 27: 531-535 https://doi.org/10.1034/j.1600-051x.2000.027007531.x
  20. Beikler T, Peters U, Prajanef S, Prior K, Ehmke B, Flemming TF. Prevalence of Porphyromonas gingivalis fimA genotypes in Caucasians. Eur J Oral Sci 2003:111:390-394 https://doi.org/10.1034/j.1600-0722.2003.00065.x
  21. Hamada S, Amano A, Kimura RK, Nakagawa I. Kawabata S, Morisaki I. The importance of the fimbriae in the virulence and etiology of some oral bacteria. Oral Microbiol Immunol 1998 : 13: 129-138 https://doi.org/10.1111/j.1399-302X.1998.tb00724.x
  22. Hamada N, Watanabe K, Arai M. Hiramine H, Umemoto T. Cytokine production induced by a 67-kDa fimbrial protein from Porphyrotnones gingivalis. Oral Microbiol Immunol 2002: 17: 197-200 https://doi.org/10.1034/j.1399-302X.2002.170311.x
  23. Sugano N, Ikeda K, Oshikawa M, Sawamoto Y, Tanaka H, Ito K. Differential cytokine induction by two types of Porphyromonas gingivalis. Oral Microbiol Immunol 2004: 19: 121-123 https://doi.org/10.1046/j.0902-0055.2003.00119.x
  24. Amano A, Sojar HT, Lee JY, Sharma A, Levin MJ, Genco RJ. Salivary receptors for recombinant fimbrillin of Porphyromonas gingivalis. Infect Immun 1994: 62: 3372-3380
  25. Amano A. Sharma A. Lee JY. Sojar HT, Raj PA Genco RJ. Structural domains of Phorphyomonas gingivalis recombinant fimbrillin that mediate binding to salivary proline-rich protein and statherin. Infect Immun 1996;64: 1631-1637
  26. Lee JY. Solar HT. Bedi GS. Genco RJ. Porphyromoas (Bacteroides) gingivalis fimbrillin: size. amino-terminal sequence. and antigenic heterogenecity. Infect Immun 1991:59:383-389
  27. Nakagawa I. Amano A, Ohara-Nemoto Y. Endoh N. Morisaki I. KImura S. Kawabata S. Hamada S. Identification of a new variant of fimA gene of Porphyromonas gingivalis and its distribution in adults and disabled population with periodontitis. J Periodontal Res 2002; 37: 425-432 https://doi.org/10.1034/j.1600-0765.2002.01637.x
  28. Amano A, Nakagawa I. Okahashi N. Hamada N. Variations of Porphyromonas gingivalis fimbriae in relation to microbial pathogenesis. J Periodontol Res 2004;39: 136-142 https://doi.org/10.1111/j.1600-0765.2004.00719.x
  29. Amano A, Kuboniwa M. Nakagawa I. Akiyama S. Morisaki I. Hamada S. Prevalence of specific genotypes of Porphyromonas gingivalis fimA and periodontal health status. J Dent Res 2000;79: 1664-1668 https://doi.org/10.1177/00220345000790090501
  30. Amano A, Nakagawa I. Kataoka K. Morisaki I. Hamada S. Distribution of Porphyromonas gingivalis strains with fimA genotypes in periodontitis patients. J Clin Microbiol 1999; 37: 1426-1430
  31. Nakagawa I. Amano A, Kimura RK. Nakamura T. Kawabata S. Hamada S. Distribution and molecular characterization of Porphyromonas gingivalis carrying a new type of fimA gene. J Clin Microbiol 2000; 38: 1909-1914
  32. 신승일, 권영혁, 박준봉, 허익. 임플란트주위염시 Porphyromonas gingivalis 섬모유전형의 출현율. 2003(unpublished)
  33. Gouvoussis J. Sindhusake D, Yeung S. Cross-infection from periodontitis sites to failing implant sites in the same mouth. Int J Oral Maxillofac Implants 1997; 12:666-673
  34. Brägger U, Aeschlimann S, Burgin W, Hammerle HFC, Lang NP. Biological and technical complications and failures with fixed partial denture (FPD) on implants and teeth after four to five years of function. Clin Oral Impl Res 2001: 12: 26-34 https://doi.org/10.1034/j.1600-0501.2001.012001026.x
  35. Mombelli A. Lang NP. Clinical parameters for the evaluation of dental implants. Periodontol 2000 1994:4:81-86 https://doi.org/10.1111/j.1600-0757.1994.tb00008.x
  36. Hamada S, Fujiwara T, Morishima S, Takahashi I. Nakagawa I. Kimura S, Ogawa T. Molecular and immunolocial charaterization of the fimbriae of Porpbyromonas gingivalis. Microbiol Immunol 1994;38:921-930 https://doi.org/10.1111/j.1348-0421.1994.tb02148.x
  37. Hamada S, Fujiwara T, Morishima S, Takahashi I, Nakagawa I, Kimura S, Ogawa T. Molecular and immunolocial charaterization of the fimbriae of Potphyromonas gingivalis. Microbiol Immunol 1994:38:921-930 https://doi.org/10.1111/j.1348-0421.1994.tb02148.x
  38. Neiders NE, Chen PB, Suido H. Reynolds HS, Zambon JJ, Shlossman M. Genco RJ. Heterogeneity of virulence among strains of Bacteroides gingivalis. J Periodont Res 1989;24: 192-198 https://doi.org/10.1111/j.1600-0765.1989.tb02005.x
  39. Nakagawa I. Amano A. Kuboniwa M. Nakamura T, Kawabata S, Hamada S. Functional differences among fimA variants of Porphyromonas gjngivaljs and their effects on adhesion to and invasion of human epithelial cells. Infect Immun 2002:70:277-285 https://doi.org/10.1128/IAI.70.1.277-285.2002
  40. Van Steenbergen TJM. Kastelein P, Touw JJ, de Graaff J. Virulence of black-pigmented Bacteroides strains from periodontal pockets and other sites in experimentally induced skin lesions in mice. J Periodont Res 1982; 17:41-49 https://doi.org/10.1111/j.1600-0765.1982.tb01129.x