Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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The cytokine-inducing activities of surface components of the periodontopathogenic bacterium Porphyromonas gingivalis

Porphyromonas gingivalis의 각종 표면성분이 싸이토카인 형성에 미치는 영향

  • Kim, Sung-Jo (Department of Periodontology, College of Dentistry, Pusan National University)
  • 김성조 (부산대학교 치과대학 치주과학교실)
  • Published : 2005.09.30
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Abstract

This study was carried out to examine the potency of the three surface compo- nents from Porphyromonas gingivalis to stimulate the murine macrophage cell line RAW264.7 to synthesize the pro-inflammatory cytokine tumor necrosis factor alpha($TNF-{\alpha}$) and nitric oxide (NO). Lipopolysaccharide(LPS). lipid A-associated proteins(LAP) and saline-extractable surface -associated material(SAM) were isolated from P. gingivalis 381. $TNF-{\alpha}$ release into culture supernatants was determined by two-site ELISA. NO production was assayed by measuring the accumulation of nitrite in culture supernatants. Western blot analysis of iNOS and analysis of reverse transcription(RT)-PCR products were carried out. The surface components extracted from this bacterium were almost equally potent in stimulating release of $TNF-{\alpha}$ and NO by RAW264.7 cells. $TNF-{\alpha}$ that was being measured immunologically was due to activation of $TNF-{\alpha}$ gene transcription. The present study clearly shows that P. gingivalis surface components fully induced iNOS expression in RAW264.7 cells in the absence of other stimuli. The ability of P. gingivalis surface components to promote the production of $TNF-{\alpha}$ and NO may be important in the pathogenesis of inflammatory periodontal disease.

Keywords

References

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