Journal of Periodontal and Implant Science

  • 제35권3호
  • /
  • Pages.675-685
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  • 2005
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  • 2093-2278(pISSN)
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  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
권호 표지

Treponema lecithinolyticum lipopolysaccharide에 의한 matrix metalloproteinase-9의 발현

Effect of Treponema lecithinolyticum lipopolysaccharide on matrix metalloproteinase-9 expression

  • 남정아 (연세대학교 치과대학 구강생물학교실) ;
  • 문선영 (연세대학교 치과대학 구강생물학교실) ;
  • 이진욱 (연세대학교 치과대학 구강생물학교실) ;
  • 차정헌 (연세대학교 치과대학 구강생물학교실) ;
  • 최봉규 (서울대학교 구강악안면 감염 면역학) ;
  • 유윤정 (연세대학교 치과대학 구강생물학교실)
  • Nam, Jeong-Ah (Dept of Oral Biology, College of Dentistry, Yonsei University) ;
  • Moon, Sun-Young (Dept of Oral Biology, College of Dentistry, Yonsei University) ;
  • Lee, Jin-Wook (Dept of Oral Biology, College of Dentistry, Yonsei University) ;
  • Cha, Jeong-Heon (Dept of Oral Biology, College of Dentistry, Yonsei University) ;
  • Choi, Bong-Kyu (Dept of Oromaxillofacial Infection and Immunity, and Dental Research Institute, College of Dentistry, Seoul National University) ;
  • Yoo, Yun-Jung (Dept of Oral Biology, College of Dentistry, Yonsei University)
  • 발행 : 2005.09.30
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초록

Bone resorption involves sequential stages of osteoclast precursor migration and differentiation of osteoclast precursors into multinucleated osteoclasts. Stromal cell derived factor (SDF)-1 is a chemotactic factor for osteoclast precursor migration. Matrix metalloproteinase (MMP)-9 is involved in migration of osteoclast precursors and activation of $interleukin(IL)-1{\beta}$. Alveolar bone destruction is a characteristic feature of periodontal disease. Treponema lecithinolyticum is a oral spirochete isolated from the periodontal lesions. The effect of lipopolysaccharide(LPS) from T. lecithinolyticum on expression of SDF-1 and MMP-9 was examined in cocultures of bone marrow cells and osteblasts derived from mouse calvariae. T. lecithinolyticum LPS increased expression of MMP-9 in the coculture. Polymyxin B, an inhibitor of LPS, abolished the increase of MMP-9 mRNA expression by LPS. LPS did not increase the expression of SDF-1, $IL-1{\beta}$ and tumor necrosis $factor(TNF)-{\alpha}$ mRNA in cocultures. Prostaglandin $E_2(PGE_2)$ up-regulated the expression of MMP-9 and NS398, an inhibitor of $PGE_2$ synthesis, down-regulated the induction of MMP-9 expression by T. lecitbinolyticm LPS. These results suggest that T. lecitbinolyticm LPS increases MMP-9 expression in bone cells via $PGE_2$ and that the induction of MMP-9 expression by T. lecitbinolyticm LPS is involved in alveolar bone destruction of periodontitis patients by the increase of osteoclast precursor migration and the activation of bone resorption-inducing cytokine.

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