Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Effect of the combined use of bone morphogenetic protein and platelet-derived growth factor on bone formation in nude mouse

누드마우스에서 골 형성에 대한 BMP와 PDGF 복합사용의 효과

  • Lee, Seoung-Ho (Department of Periodontology, Graduate School of Clinical Dentistry, Ewha Woman's University) ;
  • Choi, Byung-Ho (Department of Dentistry, Wonju Christian Hospital, Yonsei University) ;
  • Zhu, Shi-Jiang (Department of Dentistry, Wonju Christian Hospital, Yonsei University) ;
  • Huh, Jin-Young (Department of Dentistry, Gangneung Asan Hospital, University of Ulsan) ;
  • Jung, Jae-Hyung (Department of Dentistry, Ilsan Hospital) ;
  • Kim, Byung-Yong (Mi plus dental clinic, Seoul)
  • 이승호 (이화여자대학교 임상치의학대학원 치주과학교실) ;
  • 최병호 (원주의과대학 치과학교실) ;
  • 주석강 (원주의과대학 치과학교실) ;
  • 허진영 (울산대학교 의과대학 강릉아산병원 치과) ;
  • 정재형 (일산병원 치과) ;
  • 김병용 (서울 미플러스치과의원)
  • Published : 2005.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Bone morphogenetic protein(BMP) and platelet-derived growth factor(PDGF) have been demonstrated tostimulate bone formation when applied locally in vivo. To explore whether or not the combined use of BMP and PDGF could have promotive effect and synergic interaction on bone formation in vivo, bone marrow mesenchymal stem cells were treated with BMP-2, PDGF-BB, or BMP-2 plus PDGF-BB, and then these cells were injected into the subcutaneous space on the dorsum of nude mice. The bone formation was evaluated after 12 weeks. Histomorphometric analysis demonstrated that the subcutaneous nodules formed in nude mice contained 25.3% newly formed bone in the BMP-2 treated cells, 14.4% newly formed bone in the PDGF-BB treated cells, and 8.9% newly formed bone in the RMP-2 plus PDGF-BB treated cells. The results showed that the combination of BMP-2 and PDGF-BB had neither a promotive effect nor synergic interact on bone formation in vivo.

Keywords

References

  1. Bostrom MPG. Expressions of bone morphogenetic proteins in fracture healing. Clin Orthop 1998:355 (Suppl) :S116-S123
  2. Canalis E. McCarthy TL. Centrella M. Effects of platelet-derived growth factor on bone formation in vitro. J Cell Physiol 1989;140:530-537 https://doi.org/10.1002/jcp.1041400319
  3. Cassiede P. Dennis JE. Ma F, Caplan AI. Osteochondrogenic potential of marrow mesenchymal progenitor cells exposed to TGF-beta 1 or PDGF-BB as assayed in vivo and in vitro. J Bone Miner Res 1996;11:1264-1273
  4. Dennis JE. Haynesworth SE, Young RG, Caplan AI. Osteogenesis in marrow-derived mesenchymal stem cells/porous ceramic composites transplanted subcutaneously: effect fibronectin and laminin on cell retention and rate of osteogenesis expression. Cell Transplant 1992;1:23-32
  5. Fiedler J. Roderer G, Gunther KP, Brenner RE. BMP-2, BMP-4, and PDGFBB stimulate chemotactic migration of primary human mesenchymal progenitor cells. J Cell Biochem 2002;87:305-312 https://doi.org/10.1002/jcb.10309
  6. Hollinger JO, Leong K. Polyt(a-hydroxy acids) : carriers for bone morphogenetic proteins. Biomaterials 1996;17:187-194 https://doi.org/10.1016/0142-9612(96)85763-2
  7. Lee YM, Park YJ, Lee SJ, Ku Y. Han SB, Klokkevold PR. Chung CP. The bone regenerative effect of plateletderived growth factorBB delivered with a chitosan/tricalcium phosphate sponge carrier. J Periodontol 2000;71:418-424 https://doi.org/10.1902/jop.2000.71.3.418
  8. Lynch SE, Nixon JC, Colvin RB, Antoniades HN. Role of platelet-derived growth factor in wound healing: synergistic effects with other growth factors. Proc Natl Acad Sci USA 1987;84:7696-7700 https://doi.org/10.1073/pnas.84.21.7696
  9. Lynch SE, Colvin RB, Antoniades HN. Growth factors in wound healing. J Clin Invest 1989;84:640-646 https://doi.org/10.1172/JCI114210
  10. Maniatopoulos C, Sodek J, Mekcher AH. Bone formation in vitro by stromal cells obtained from marrow of young adults. Cell Tissue Res 1988;254:317-330
  11. Mitlak BH. Finkelman RD, Hill EL, Li J, Martin B, Smith T. The effects of systemically administered PDGF-BB on the rodent skeleton. J Bone Miner Res 1996;11:238-247
  12. Noshi T, Yoshikawa T, Dohi Y, Ikeuchi M. Horiuchi K. Ichiiima K. Sugimura M. Yonemasu K. Ohgushi H. Recombinant human bone morphogenetic protein-2 potentiates the in vivo osteogenic ability of marrow/hydroxyapatite composites. Artif Organs 2001;25:201-208 https://doi.org/10.1046/j.1525-1594.2001.025003201.x
  13. Onishi T, Ishidou Y, Nagamine T. Distinct and overlapping patterns of localization of bone morphogenetic protein (BMP) family members and a bmp type II receptor during fracture healing in rats. Bone 1998;22:605-612 https://doi.org/10.1016/S8756-3282(98)00056-8
  14. Pierce GF, Vande Berg J, Rudolph R, Tarpley J, Mustoe TA. Platelet-derived growth factor BB and transforming growth factor beta 1 selectively modulate glvcosaminoglycans, collagen, and myofibroblasts in excisional wounds. Am J Pathol 1991;138:629-646
  15. Roberts AB, Anzano MA, Wakefield LM, Roche NS, Stern DF, Sporn MB. Type beta transforming growth factor: a bifunctional regulator of cellular growth. Proc Natl Acad Sci USA 1985;82:119-123 https://doi.org/10.1073/pnas.82.1.119
  16. Schmitt JM, Hwang K, Winn SR, Hollinger JO. Bone morphogenetic proteins: an update on basic biology and clinical relevance. J Orthop Res 1999;17:269-278 https://doi.org/10.1002/jor.1100170217
  17. Shimakura Y, Yamzaki Y, Uchinuma E. Experimental study on bone formation potential of cryopreserved human bone marrow mesenchymal cell/hydroxyapatite complex in the presence of recombinant human bone morphogenetic protein-2. J Craniofac Surg 2003;14:108-116 https://doi.org/10.1097/00001665-200301000-00021
  18. Sporn ME, Roberts AB, Wakefield LM, Assoian RK. Transforming growth factor beta: biological function and chemical structure. Science 1986;233:532-534 https://doi.org/10.1126/science.3487831
  19. Vehof JW, Mahmood J, Takita H. Ectopic bone formation in titanium mesh loaded with bone morphogenetic protein and coated with calcium phosphate. Plast Reconstr Surg 2001;108:434-443 https://doi.org/10.1097/00006534-200108000-00024
  20. Vikjaer D, Blom S, Hiorting-Hansen E, Pinholt EM. Effect of platelet-derived growth factor-BB on bone formation in calvarial defects: an experimental study in rabbits. Eur J Oral Sci 1997;105:59-66 https://doi.org/10.1111/j.1600-0722.1997.tb00181.x
  21. Zhang L, Leeman E, Carnes DC, Graves DT. Human osteoblasts synthesize and respond to platelet-derived growth factor. Am J Physiol 1991;261:C348-C354