Effect of Liquid Circulation Velocity and Cell Density on the Growth of Parietochloris incisa in Flat Plate Photobioreactors

  • Changhai Wang (Institute of Marine Biochemical Engineering, Yantai University, Marine Biochemical Engineering Lab., Dalian University of Technology) ;
  • Yingying Sun (Marine Biochemical Engineering Lab., Dalian University of Technology) ;
  • Ronglian Xing (Marine Biochemical Engineering Lab., Dalian University of Technology) ;
  • Liqin Sun (Institute of Marine Biochemical Engineering, Yantai University, Marine Biochemical Engineering Lab., Dalian University of Technology)
  • Published : 2005.03.01

Abstract

For more accurately describing the durations of the light and the dark phases of micro-algal cells over the whole light-dark cycle, and probing into the relationship between the liquid circulation time or velocity, the aeration rate and cell density, a series of experiments was carried out in 10 cm light-path flat plate photobioreactors. The results indicated that the liquid flow in the flat plate photobioreactor could be described by liquid dynamic equations, and a high biomass output, higher content and productivity of arachidonic acid, $70.10\;gm^{-2}d^{-1},\;9.62\%$ and 510.3 mg/L, respectively, were obtained under the optimal culture conditions.

Keywords

References

  1. Richmond, A. (1999) Physiological principles and modes of cultivation in mass production of photoautotrophic microalgae. pp. 353-386. In: Cohen, Z. (ed.). Chemical from Microalgae. Taylor & Francis Ltd
  2. Molina Grima, E., J. A. Sanchen Perez, F. Garcia Camacho, J. M. Fernandez Sevilia, and F. G. Acien Fernandez (1997) Productivity analysis of outdoor chemostat culture in tubular air-lift photobioreactors. J. Appl. Phycol. 8: 369-380 https://doi.org/10.1007/BF02178580
  3. Richmond, A. and A. Vonshak (1978) Spirulina culture in Israel. Arch. Hydrobiol. Beih. Ergebn. Limnol. 11: 274-280
  4. Markl, H. (1980) Modeling of algal production systems. pp. 361-383. In: Shelef, G. and C. J. Soeder (eds.). Algae Biomass. Elsevier/Noth-Holland Biomedical Press
  5. Erikson, L. E. and H. Y. Lee (1986) Process analysis and design of algal growth systems. pp. 197-206. In: Barclay W. and R. Mcintosh (eds.). Algal Biomass Technologies. Nova Hedwigia
  6. Hu, Q. and A. Richmond (1996) Productivity and photosynthetic efficiency of Spirulina platensis as affected by light intensity, algal density and rate of mixing in a flat plate photobioreactor. J. Appl. Phycol. 8: 139-145 https://doi.org/10.1007/BF02186317
  7. Hu, Q. and A. Richmond (1996) Productivity and photosynthetic efficiency of Spirulina platensis as affected by light intensity, algal density and rate of mixing in a flat plate photobioreactor. J. Appl. Phycol. 8: 139-145 https://doi.org/10.1007/BF02186317
  8. Hu, Q., Y. Zarmi, and A. Richmond (1998) Combined effects of light intensity, light-path and culture density on output rate of Spirulina platensis (Cyanobacteria). Eur. J. Phycol. 33: 165-171 https://doi.org/10.1080/09670269810001736663
  9. Wang, C. H. (1998) Microalgae Culture in Photobioreactor. Ph.D. Thesis. State Key Laboratory of Biochemical Engineering, Chinese Academy of Science, China
  10. Wang, C. H. and F. Ouyang (2000) The cultures of Porphyridium cruentum in photobioreactor. Engineering Chemistry Metallurgy (China) 21: 47-51
  11. Bosca, C., A. Dauta, and O. Marvalin (1991) Intensive outdoor algal cultures: How mixing enchces the photosynthetic production rate. Bioresour. Technol. 38: 185-188 https://doi.org/10.1016/0960-8524(91)90152-A
  12. Laws, E. A., K. L. Terry, J. Wickman, and M. S. Challup (1983) A simple algal production system designed to utilize the flashing light effect. Biotechnol. Bioeng. 25: 2319-1335 https://doi.org/10.1002/bit.260251004
  13. Richmond, A. and J. U. Grobbelaar (1986) Factors affecting the output rate of Spirulina platensis with reference to mass cultivation. Biomass 10: 253-263 https://doi.org/10.1016/0144-4565(86)90002-8
  14. Hu, Q., H. Guterman, and A. Richmond (1996) A flat inclined modular photobioreactor for outdoor mass cultivation of photoautotrophs. Biotechnol. Bioeng. 51: 51-60 https://doi.org/10.1002/(SICI)1097-0290(19960705)51:1<51::AID-BIT6>3.0.CO;2-#
  15. Kok, B. (1953) Experiments on photosynthesis by Chlorella in flashing light. pp. 63-75. In: Burlew, J. S. (ed.). Algal Culture: From Laboratory to Pilot Plant. Carnegie Institute of Washington, Washington Pub., USA
  16. Laws, E. R., K. L. Terry, J. Wickman, and M. S. Challup (1983) A simple algal production system designed to utilize the flashing light effect. Biotech. Bioeng. 25: 2319-2335 https://doi.org/10.1002/bit.260251004
  17. Lee, Y. K. and S. J. Pirt (1981) Energetic of photosynthetic algal growth: Influence of intermittent illumination in short (40s) cycles. J. Gen. Microbiology 124: 43-52
  18. Terry, K. L. (1986) Photosynthesis in modulated light:quantitative dependence of photosynthetic enhancement on flashing rate. Biotechnol. Bioeng. 28: 988-995 https://doi.org/10.1002/bit.260280709
  19. Wen, S. H. and C. H. Wang (2001) Study on the light decline model of Porphyridium cruentum. Marine Science Bulletin 20: 35-39
  20. Wang, C. H. and F. Ouyang (1999) Effect of irradiance and temperature on the growth of Spirulina platensis. Engineering Chemistry Metallurgy (China) 20: 371-375