Archives of Pharmacal Research

  • 제28권4호
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  • Pages.421-432
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  • 2005
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  • 0253-6269(pISSN)
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  • 1976-3786(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Reabsorption of Neutral Amino Acids Mediated by Amino Acid Transporter LAT2 and TAT1 in The Basolateral Membrane of Proximal Tubule

  • Park Sun Young (Department of Pediatrics, Kwangju Christian Hospital) ;
  • Kim Jong-Keun (Department of Pharmacology, Chonnam National University Medical School) ;
  • Kim In Jin (Department of Oral Physiology, Chosun University College of Dentistry) ;
  • Choi Bong Kyu (Department of Pharmacology, Wonkwang University School of Medicine) ;
  • Jung Kyu Yong (Department of Pharmacology, Wonkwang University School of Medicine) ;
  • Lee Seoul (Department of Pharmacology, Wonkwang University School of Medicine) ;
  • Park Kyung Jin (Department of Oral Physiology, Chosun University College of Dentistry) ;
  • Chairoungdua Arthit (Department of Pharmacology and Toxicology, Kyorin University School of Medicine) ;
  • Kanai Yoshikatsu (Department of Pharmacology and Toxicology, Kyorin University School of Medicine) ;
  • Endou Hitoshi (Department of Pharmacology and Toxicology, Kyorin University School of Medicine) ;
  • Kim Do Kyung (Department of Oral Physiology, Chosun University College of Dentistry)
  • 발행 : 2005.04.01
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초록

In order to understand the renal reabsorption mechanism of neutral amino acids via amino acid transporters, we have isolated human L-type amino acid transporter 2 (hLAT2) and human T-type amino acid transporter 1 (hTAT1) in human, then, we have examined and compared the gene structures, the functional characterizations and the localization in human kidney. Northern blot analysis showed that hLAT2 mRNA was expressed at high levels in the heart, brain, placenta, kidney, spleen, prostate, testis, ovary, lymph node and the fetal liver. The hTAT1 mRNA was detected at high levels in the heart, placenta, liver, skeletal muscle, kidney, pancreas, spleen, thymus and prostate. Immunohistochemical analysis on the human kidney revealed that the hLAT2 and hTAT1 proteins coexist in the basolateral membrane of the renal proximal tubules. The hLAT2 transports all neutral amino acids and hTAT1 transports aromatic amino acids. The basolateral location of the hLAT2 and hTAT1 proteins in the renal proximal tubule as well as the amino acid transport activity of hLAT2 and hTAT1 suggests that these transporters contribute to the renal reabsorption of neutral and aromatic amino acids in the basolateral domain of epithelial proximal tubule cells, respectively. Therefore, LAT2 and TAT1 play essential roles in the reabsorption of neutral amino acids from the epithelial cells to the blood stream in the kidney. Because LAT2 and TAT1 are essential to the efficient absorption of neutral amino acids from the kidney, their defects might be involved in the pathogenesis of disorders caused by a disruption in amino acid absorption such as blue diaper syndrome.

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