생명과학회지 (Journal of Life Science)

  • 제15권2호
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  • Pages.287-292
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  • 2005
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Trichoderma erinaceum DG-312 신규 곰팡이 균주로부터 생산된 galactomannan의 항염증 활성

Anti-inflammatory Activity of the Galactomannans Produced by a New Fungal Strain Trichoderma erinaceum DG-312

  • 주지훈 (대구대학교 공과대학 생물공학과) ;
  • 임종민 (대구대학교 공과대학 생물공학과) ;
  • 구광본 (대구대학교 생명환경대학 생명산업학과) ;
  • 윤종원 (대구대학교 공과대학 생물공학과) ;
  • 최장원 (대구대학교 생명환경대학 생명산업학과)
  • Joo Ji-Hoon (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Lim Jong-Min (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Koo Kwang-Bon (Department of Bioindustry, College of Life & Environment, Daegu University) ;
  • Yun Jong-Won (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Choi Jang Won (Department of Bioindustry, College of Life & Environment, Daegu University)
  • 발행 : 2005.04.01
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초록

새로 분리된 곰팡이 Trichoderma erinaceum DG-312의 균사체 배양에 의해 갈락토만난(GMs)을 생산하여 쥐에서 항염증 활성을 조사하였다. 5 리터 교반 발효조에서 3일 동안 배양에 의해 얻어진 균사체 및 갈락토만난의 최대 농도는 각각 9.44 g/l 및 2.72 g/l 이였다. Sepharose CL-6B 젤 크로마토그라피와 다당체 성분 분석 결과, crude 갈락토만난의 조성은 주로 mannose $(74.9\%)$와 galatose $(24.1\%)$로 구성된 이종의 다당체인 것으로 밝혀졌다. Trichoderma erinaceum DC-312로부터 생산된 갈락토만난은 아세트산으로 처리한 쥐에서 체중(kg)에 대하여 100 또는 200 mg의 양으로 처리하였을 때 농도에 비례하여 현저한 항 염증 활성을 갖는 것으로 나타났다. 갈락토만난의 항염증 활성을 나타내는 모세혈관 투과도(vascular permeability) 및 발작 반응(writhing response)의 저해 수치는 각각 $60.6\%$ 및 $62.5\%$로 나타났다. 따라서 이런 갈락토만난의 항염증 및 발작반응 저해에 효과적인 특성을 이용한다면 치료 목적을 위한 의약물질 개발의 잠재성을 보여준다.

The crude galactomannans (GMs) were obtained from the culture of a newly isolated fungus Trichoderma erinaceum DG-312 and their anti-inflammatory activity was investigated in mice. The maximum concentrations of mycelial biomass and GMs reached 9.44 g/l, 2.72 g/l at day 3 in a 5-l stirred-tank bioreactor, respectively. The results of Sepharose CL-6B gel chromatography and compositional analysis revealed that the crude GMs contain heterogeneous polysaccharides consisting of $74.9\%$ mannose and $24.1\%$ galactose. The GMs was shown to possess a significant anti-inflammatory activity against acetic acid-induced inflammatory mouse model in a dose-dependent manner, when mice were treated with 100 and 200 mg GMs/kg body weight. The inhibition in vascular permeability $(60.6\%)$ and in writhing response $(62.5\%)$ evidenced an anti-inflammatory activity of the GMs. The marked anti-inflammatory and writhing-lowering properties of the GMs suggest its potential therapeutic use.

키워드

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