생명과학회지 (Journal of Life Science)

  • 제15권2호
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  • Pages.236-241
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  • 2005
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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미역취뿌리 추출물이 성장기 흰쥐의 골대사에 미치는 영향

The Effects of Solidago virga-aurea var. gigantea Miq. Root Extract on Bone Metabolism in Growth Period Rats

  • 이지원 (계명대학교 식품가공학과) ;
  • 박정현 (계명대학교 전통 미생물자원 개발 및 산업화 연구센터) ;
  • 이효주 (계명대학교 식품가공학과) ;
  • 이인선 (계명대학교 전통 미생물자원 개발 및 산업화 연구센터)
  • Lee Ji-Won (Dept. of Food Science & Technology, Keimyung University) ;
  • Park Jung-Hyun (The Center for Traditional Microorganism Resources center, Keimyung University) ;
  • Lee Hyo-Joo (Dept. of Food Science & Technology, Keimyung University) ;
  • Lee In-Seon (The Center for Traditional Microorganism Resources center, Keimyung University)
  • 발행 : 2005.04.01
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초록

본 연구에서는 3주령의 암컷 흰쥐를 이용하여 미역취뿌리 추출물이 골대사에 미치는 영향을 조사하고자 미역취뿌리 메탄올 추출물을 10, 50, 100 mg/kg/day의 농도로 음용수에 희석하여 9주간 사육시켰다. 매주 체중과 사료효율을 측정한 결과, 대조군에 비해 체중의 변화나 사료효율에는 유의적으로 차이가 없는 것으로 보아 미역취 뿌리 추출물에 대한 기본적 독성은 없고, 성장에 미치는 영향은 없는 것으로 사료되었다. 그리고 3주 간격으로 소변으로 배출되는 Ca과 P의함량을 ICP로 분석 한 결과, 10 mg/kg/day농도로 음용한 군에서 유의적으로 감소됨을 확인 할 수 있었고 혈액으로 유리되는 Ca과 P의 함량 또한 섭취군에서 유의적으로 감소되는 것을 확인하였다. 또한 섭취 후 9주째에 양에너지 방사선 골밀도 측정기(dual energy x-ray absorptiometry, DEXA)를 이용하여 척추, 대퇴골, 골반의 골밀도와 골무기질 함량을 측정한 결과, 메탄을 추출물을 음용한 군의 척추 골밀도가 대조군에 비해 유의적으로 높게 나타났으며, 글 무기질 함량 또한 대퇴골과 골반에서 대조군에 비해 유의적으로 높게 나타났다. 따라서 미역취뿌리 메탄을 추출물의 음용이 암컷 흰쥐에서 골밀도 및 골함량에 유의하게 작용하고 또한 골대사 과정에서 유리되는 Ca와 P의 대사과정에 작용하여 유리되는 Ca과 P의 양을 감소시켜 이상의 결과로 미뤄볼 때, 적절한 미역취뿌리 추출물의 투여는 흰쥐의 성장에 대해 부작용이나 독성없이 골밀도와 골무기질 함량을 높였으며 앞으로 조골세포 기능 저하로 인한 골대사 질환에 사용되어 질 수 있을 것이다.

To investigate the bioactivities of Solidago virga-aurea var. gigantea Mig. Root (SVR), we studied the effect of a SVR methanol extract on the activity of bone metabolism. Spraque-Dawley three-week-old female rats were randomly assigned to groups as follows : non-supplemented rats and supplemented with SVR at 10, 50, 100 mg/kg bw/day. Every week determined weight gain and food intake, urine and blood examination of mineral content of calcium and phosphorus was performed each at experimental periods of 3 and 9 weeks respectively; bone mineral density and bone mineral content were also assayed. There were no significant differences in body weight or feed efficiency ratio levels. However, the biological value of calcium and phosphorus excretion in the group supplemented with SVR extract decreased significantly more than that in the group not supplemented with SVR extract. Also, spine BMD, femur BMC and pelvis BMC per weight were significantly greater on SVR extract supplemented groups than that of the control group. In conclusion, it might be expected that methanol extract of SVR does not impair the growth of rats and may improve bone metabolism in rats.

키워드

참고문헌

  1. Boonen, A., P. Broos and J. Deqeker. 1997. The prevention of treatment of age-related osteoporosis in the elderly by systemic recombinant growth factor therapy(rhIGF-I or rhRGF-$\beta$):a perspective. J. internal medicine 242, 285-290 https://doi.org/10.1046/j.1365-2796.1997.00133.x
  2. Chae, J. H. 2001. The effects of isoflavones on bone mineral density and bone mineral content in growing male rats. Master thesis, Keimyung University, Daegu, Korea
  3. Cho, S. H., K. G, Kim, S. R. Kim, J. A. Lee, H. Moon and Y. Y. Hwang. 1996. The effects of 17-$\beta$) etradiol, medroxyprogesterone acetate and parathyroid hormone on the differentiation of osteoblast cell. Korean Soc. Obst. Gyn. 39, 1497-1506
  4. Choi, K. H., S. E. Hong, Y. J. Seo and B. R. Park. 1999. Effects of hominis placenta on the ovariectomized rat model of postmenopausal osteoporosis. The J Oriental Gynecology 12, 75-100
  5. Choi, M. J. 2002. Effects of soy protein on bone mineral content and bone mineral density in growing male rats. J. Korean Soc. Food Sci. Nutr. 35, 409-413
  6. Glajchen, N., S. Thomas, P. Jowell, S. Epstein, F. Ismail and M. Fallon. 1990. The effect of high-dose salmon clacitonin on bone mineral metabolism in the normal rat. Calcif Tissue Int. 46, 28-32 https://doi.org/10.1007/BF02555821
  7. Goswami. A., R. N. Barua, R. P. Sharma, J. N. Baruah and P. Kulanthaivel 1984. Clerodanes from Solidago virga-aurea. Phytochemistry 23, 837-841 https://doi.org/10.1016/S0031-9422(00)85038-4
  8. Ha, K. S. 1996. Effect of hormones and dietary calcium levels on bone metabolism of mouse. Master thesis, Sookmyung women's universtiy, Seoul, Korea
  9. Hansen, M. A., K. Overgaard, B. J. Riis and C. Christiansen. 1991. Role of peak bone mass and bone loss in postmenopausal osteoporosis:12year study. Br Med J. 303, 961-964 https://doi.org/10.1136/bmj.303.6808.961
  10. Hawker, G. A. 1996. The epidemiology of osteoporosis. J Rheumatol suppl. 45, 2-5
  11. Hwang, J.B., M. O. Yang and H. K. Shin. 1997. Suevey for approximate composition and mineral content of medicinal herbs. J. Food Sci. 29, 671-679
  12. Jung, S. H. 1994. The effect of dietary protein source and sulfur amino acid content on bone metabolism in male rats. Master Thesis, Keimyung University, Daegu, Korea
  13. Kalu, D. N., R. Cockerham, B. P. Yu and B. A Roos. 1983. Lifelong dietary modulation of calcitonin levels in rats. Endocrinology 113, 2010-2016 https://doi.org/10.1210/endo-113-6-2010
  14. Kalu, D. N., R. H. Hardin, R. Cockerham and B. P. Yu. 1984. Agin and dietary modulation of rat skeleton and parathyroid hormone. Endocrinology 115, 1239-1247 https://doi.org/10.1210/endo-115-4-1239
  15. Kim, H. S. 1996. Study for volatile compounds, antimicrobial and antioxidant of Solidago oirga-au rea and Solidago virgaaurea var. coreana NAKAI. Master thesis, Busan Universtiy, Busan, Korea
  16. Kim, H. W and B. H. Ahn. 2001. A comparative study of tricalcium phosphate and gelatin on osteogenesis. Korean Aca Oral Biol. 86, 255-269
  17. Kim, M. R., C. H. Yang and B. I. Seo. 1998. Effect of safflower seeds on bone mineral density in ovariectomyinduced postmenopausal osteoporotic rats. Kor. J. herbology. 13, 37-43
  18. Kim, N. S., Y. S. You, C. W. Kang and I. H. Choi. 2000. The changes of osteocalcin, bone-specific alkaline phosphatase, estrogen, IGF-I, Ca2+, P and bone mineral density on osteoporosis induced by ovariectomy in rats. Korea J. vet. Res. 40, 755-762
  19. Kim, T. J. 1997. Wild flowers of korea. Kugil media, Seoul, Korea
  20. Lee, J. A. 1992. Effects of various heat-treated milks on growth, protein and calcium metabolism of rats. Master thesis, Chonnam National University, Kwang ju, Korea
  21. Lee, Y. G., J. S. Choi, S. S. Seo, K. M. Kong and J. W. Kim. 1998. The effect of recombinant human growth hormone on prevention of osteoporosis in ovariectomized rat. J. of Korean Orthop. Assoc. 33, 1941-1951
  22. Lee, Y. M., S. M. Choi, Y. J. Park, S. J. Lee, Y. Ku and C. P. Chung. 1998. Tissue engineered bone formation using porous chitosan and chitosan/tricalcium phosphate matrices. J. Periodont. Res. 28, 577-604
  23. Maskarinec, G., S. Singh, L. Meng and A. A. Franke. 1998. Dietary soy intake and urinary isoflavone excretion among women from a multi-ethic population. Cancer Epidemiology Biomarkers & Prevention. 7, 613-619
  24. Oh, H. S. H. C. Kim, S. I. Lee and D. K. Ahn. 1995. Effects of eucommiae cortex and folium on the ovariectomized rat as the model of postmenopaual osteoporosis. J. of Herbology 10, 59-68
  25. Ott, S. M. 1990. Attainment of peak bone mass. J Clin Endoclinol Metab. 71, 1082 https://doi.org/10.1210/jcem-71-5-1082
  26. Shiraki, M and H. Orimo. 1991. The effect of estrogen and sex-steroids and thyroid hormone preparation on bone mineral density in senile osteoporosis - a comparative study of the effect of 1 alpha-hydroxy-cholecalciferol in ssenile osteoporosis. Nippon Naibupi Gakkai Zasshi. 67, 84-95
  27. Sung, J. H., J. O. Lee, J. K. Son, N. S. Park, M. R. Kim, J. G. Kim and D. C. Moon. 1999. Cytotoxic constituents from Solidago virga-au rea var. gigantea Miq. Arch Pharm Res. 22, 633-637 https://doi.org/10.1007/BF02975338
  28. The Korean Nutrition Society. 1995. Korean nutrient standard. Seoul, Korea
  29. Toshio, M., I. Yoshinori and U. Akira. 1991. Studies on the constituents of Solidago virga-aurea L.I. structural elucidation of saponins in the Herb. Chem. Pharm. Bull. 39, 2037-2042 https://doi.org/10.1248/cpb.39.2037
  30. Toshio, M., I. Yoshinori and U. Akira. 1992. Studies on the constituents of Solidago virga-au rea L.II. structures of Solidagosaponins X-XX Chem. Pharm. Bull. 40, 946-953 https://doi.org/10.1248/cpb.40.946
  31. Ziegler, R., S. N. Christa and S. Scharla. 1995. Pathophysiology of osteoporosis : unresolved problem and new insights. J. Nutr. 125, 2003-2037