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Up-regulation of Galectin-3 in HIV-1 tat-transfected Cells

  • Yu Hak Sun (Department of Parasitology, College of Medicine, Pusan National University) ;
  • Kim KoanHoi (Department of Pharmacology, College of Medicine, Pusan National University)
  • Published : 2005.04.01

Abstract

Previous studies have demonstrated that expression of galectin-3, a member of family of beta-galactoside-binding animal lectin, is associated with pathological conditions including cancer, atherosclerosis, and viral infection. An increase of this lectin has been observed after infection by Kirsten murine sarcoma, human T lymphotropic virus-l (HTLV-l), and human immunodeficiency virus-l (HIV-l). Viral transactivation protein Tax of HTLV-l mediates the increase in the lectin. In case of HIV-1, there are evidences that Tat would be related with increase in galectin-3. We investigated whether Tat directly induced galectin-3 expression in cells. We found that HIV-l tat gene activated galectin-3 promoter in RAW264.7 cells. To demonstrate direct induction of galectin-3 by HIV-l tat, we transfected the tat into a rabbit smooth muscle cell line (Rb1) and obtained RblTatCl-2, a clone of cell stably transfected with tat gene. The Rb1TatCl-2 cells exhibited activation of LTR promoter and up-regulation of galectin-3 transcript as well as protein. Our results indicate that HIV-l tat alone is sufficient to induce the expression of galectin-3. The Rb1TatCl-2 cells could be valuable for study of the effect of HIV-1 tat on expression of cellular genes.

Keywords

References

  1. Agrwal, N., J. L. Wang and P. G. Voss. 1989. Carbohydratebinding protein 35. Levels of transcription and mRNA accumulation in quiescent and proliferating cells. J. Biol. Chem. 264, 17236-17242
  2. Akahani, S., P. Nangia-Makker, H. Inohara, H. R. Kim and A. Raz. 1997. Galectin-3: a novel antiapoptotic molecule with a functional BH1 (NWGR) domain of Bcl-2 family. Cancer Res. 57, 5272-5276
  3. Albini, A., R. Soldi, D. Giunciuglio, E. Giraudo, R. Benelli, L. Primo, D. Noonan, M. Salio, G. Camussi, W. Rockl and F. Bussolino. 1996. The angiogenesis induced by HIV-1 tat protein is mediated by the Flk-1/KDR receptor on vascular endothelial cells. Nat. Med. 2, 1371-1375 https://doi.org/10.1038/nm1296-1371
  4. Barondes, S. H., D. N. W. Cooper, M. A. Gitt and H. Leffler. 1994. Galectins. Structure and function of a large family of animal lectins. J. Biol. Chem. 269, 20807-20810
  5. Buonaguro, L., G. Barillari., H. K Chang, C. A. Bohan, V. Kao, R. Morgan, R. C. Gallo and B. Ensoli. 1992. Effects of the human immunodeficiency virus type 1 Tat protein on the expression of inflammatory cytokines. J. Virol. 66, 7159-7167
  6. Castronovo, V., F. A. Van den Brule, P. Jackers, N. Clausse, F-T. Liu, C. Gillet and M. E. Sobel. 1996. Decreased expression of galectin-3 is associated with progression of human breast cancer. J. Pathol. 179, 43-48 https://doi.org/10.1002/(SICI)1096-9896(199605)179:1<43::AID-PATH541>3.0.CO;2-N
  7. Dagher, S. F., J. L. Wang and R. J. Patterson. 1995. Identification of galectin-3 as a factor in pre-mRNA splicing. Proc. Natl. Acad. Sci. U.S.A. 92, 1213-1217
  8. Fogel, S., M. Guittaut, A. Legrand, M. Monsigny and E. Hebert. 1999. The tat protein of HIV-I induces galectin-3 expression. Glycobiology 9, 383-387 https://doi.org/10.1093/glycob/9.4.383
  9. Frigeri, L. G., R. I. Zuberi and F-T. Liu. 1993. Epsilon BP, a beta-galactoside-binding animal lectin, recognizes IgE receptor (Fe epsilon RI) and activates mast cells. Biochemistry 32, 7644-7649 https://doi.org/10.1021/bi00081a007
  10. Ganju, R. K., N. Munshi, B. C. Nair, Z. Y. Liu, P. Gill and J. E. Groopman. 1998. Human immunodeficiency virus tat modulates the FIk-1/KDR receptor, mitogen-activated protein kinases, and components of focal adhesion in Kaposi's sarcoma cells. J. Virol. 72, 6131-6137
  11. Gaudin, J. C., C. Arar, M. Monsigny and A. Legrand. 1997. Modulation of the expression of the rabbit galectin-3 by p53 and Ha-ras proteins and PMA. Glycobiology 7, 1089-1098 https://doi.org/10.1093/glycob/7.8.1089
  12. Hsu, D. K., C. A. Dowling, K. C. G. Jeng, J. T. Chen, R. Y. Yang and F-T. Liu. 1999. Galectin-3 expression is induced in cirrhotic liver and hepatocellular carcinoma. Int. J. Cancer 81, 519-526 https://doi.org/10.1002/(SICI)1097-0215(19990517)81:4<519::AID-IJC3>3.0.CO;2-0
  13. Hsu, D. K., S. R. Hammes, I. Kuwabar, W. C. Greene and F-T. Liu. 1996. Human T lymphotropic virus-1 infection of human T-Iymphocytes induces expression of the betagalactoside-binding lectin, galectin-3. Am. J. Pathol. 148, 1661-1670
  14. Hsu, D. K., R. Y. Yang, Z. Pan, L. Yu, D. R. Salomon, W. P. Fung-Leung and F-T. Liu. 2000. Targeted disruption of the galectin-3 gene results in attenuated peritoneal inflammatory responses. Am. J. Pathol. 156, 1073-1083 https://doi.org/10.1016/S0002-9440(10)64975-9
  15. Konstantinov, K. N., B. A. Robbins and F-T. Liu. 1996. Galectin-3, a galactoside-binding animal lectin, is a marker of anaplastic larger-cell lymphoma. Am. J. Pathol. 148, 25-30
  16. Li, F-T. 1993. S-type mammalian lectins in allergic inflammation. Immunol. Today 14, 486-490 https://doi.org/10.1016/0167-5699(93)90263-K
  17. Liu, F-T., D. K. Hsu, R. I. Zuberi, I. Kuwahara, E. Y. Chi and W. R. Jr. Henderson. 1995. Expression and function of galectin-3, a beta-galactoside-binding lectin, in human monocytes and macrophages. Am. J. Pathol. 147, 1016-1028
  18. Lotz, M. M., C. W. Jr. Andrews, C. A. Korzelius, E. C. Lee, D. G. Jr. Steele, A. Clarke and A. M. Mercurio. 1993. Decreased expression of Mac-2 (carbohydrate binding protein 35) and loss of its nuclear localization are associated with the neoplastic progression of colon carcinoma. Proc. Natl. Acad. Sci. U.S.A. 90, 3466-3472
  19. Matarrese, P., O. Fusco, N. Tinari, C. Natoli, F-T. Liu, M. L. Semeraro, W. Malorni and S. Iacobelli. 2000. Galectin-3 overexpression protects from apoptosis by improving cell adhesion properties. Int. J. Cancer 85, 545-54 https://doi.org/10.1002/(SICI)1097-0215(20000215)85:4<545::AID-IJC17>3.0.CO;2-N
  20. Menegon, A, C. Leoni, F. Benfenati and F. Valtorta. 1997. Tat protein from HIV-1 activates MAP kinase in granular neurons and glial cells from rat cerebellum. Biochem. Biophys. Res. Commun. 238, 800-805 https://doi.org/10.1006/bbrc.1997.7393
  21. Moutsatsos, I. K., M. Wade, M. Schindler and J. L. Wang. 1987. Endogenous lectins from cultured cells: nuclear localization of carbohydrate-binding protein 35 in proliferating 3T3 fibroblasts. Proc. Natl. Acad. Sci. U.S.A. 84, 6452-6456
  22. Nachtigal, M., Z. Al-Assaad, E. P. Mayer, K. Kim and M. Monsigny. 1998 Galectin-3 expression in human atherosclerotic lesions. Am. J. Pathol. 152, 1199-1208
  23. Nachtigal, M., M. L. Nagpal, P. Greenspan, S. A. Nachtigal and A. Legrand. 1989. Characterization of a continuous smooth muscle cell line derived from rabbit aorta. In Vitro Cell Dev. Biol. 5, 892-898
  24. Nangia-Makker, P., J. Ochieng, J. K. Christman and A. Raz. 1993. Regulation of the expression of galactosidebinding lectin during human monocytic differentiation. Cancer Res. 53, 5033-5037
  25. Raz, A., D. G. Zhu, V. Hogan, N. Shah, T. Raz, R. Karkash, G. Pazerini and P. Carmi. 1990. Evidence for the role of 34-kDa galactoside-binding lectin in transformation and metastasis. Int. J. Cancer 46, 871-877 https://doi.org/10.1002/ijc.2910460520
  26. Sastry, K. J., H. R. Reddy, R. Pandita, K. Totpal and B. B. Aggarwal. 1990. HIV-1 tat gene induces tumor necrosis factor-beta (lymphotoxin) in a human B-lymphoblastoid cell line. J. Biol. Chem. 265, 20091-20093
  27. Schroder, H. C., H. Ushijima, C. Theis, A. P. Seve, J. Hubert and W. E. Muller. 1995. Expression of nuclear lectin carbohydrate-binding protein 35 in human immunodeficiency virus type 1-infected Molt-3 cells. J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. 9, 340-348
  28. Schwartz, O., J. L. Virelizier, L. Montagnier and U. Hazan. 1990. A microtransfection method using the luciferase-encoding reporter gene for the assay of human immunodeficiency virus LTR promoter activity. Gene 88, 197-205 https://doi.org/10.1016/0378-1119(90)90032-M
  29. Van den Brule, F. A., C. Buicu, A. Berchuck, R. C. Bast, M. Deprez, F-T. Liu, D. N. W. Cooper, C. Pieters, M. E. Sobel and V. Castronovo. 1996. Expression of the 67-kDa laminin receptor, galectin-1 and galectin-3 in advanced human uterine adenocarcinoma. Hum. Pathol. 27, 1185-1191 https://doi.org/10.1016/S0046-8177(96)90313-5
  30. Westendorp, M. O., M. Li-Weber, R. W. Frank and P. H. Krammer. 1994. Human immunodeficiency virus type 1 Tat upregulates interleukin-2 secretion in activated T cells. J. Virol. 68, 4177-4185
  31. Xu, X. C., A. K. El-Naggar and R. Lotan. 1995. Differential expression of galectin-1 and galectin-3 in thyroid tumors: potential diagnostic implications. Am. J. Pathol. 147, 815-822
  32. Yang, R. Y., D. K. Hsu and F-T. Liu. 1996. Expression of galectin-3 modulates T-cell growth and apoptosis. Proc. Natl. Acad. Sci. U.S.A. 93, 6737-6742