Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Plant Regeneration through Somatic Embryogenesis of Leymus chinensis Trin.

양초(Leymus chinensis Trin.)의 체세포배발생에 의한 식물체 재분화

  • Kim Myoung Duck (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Jin Hua (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Institute of Biological Resources and Environment Research. Dalian Nationalities University, China) ;
  • Park Eun-Joon (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kwon Suk-Yoon (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee Haeng-Soon (Laboratory of Plant Cell Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kwak Sang-Soo (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 김명덕 (한국생명공학연구원 환경생명공학연구실) ;
  • 김화 (한국생명공학연구원 환경생명공학연구실, 중국 대련민족대학 생물자원환경연구소) ;
  • 박은준 (한국생명공학연구원 환경생명공학연구실) ;
  • 권석윤 (한국생명공학연구원 환경생명공학연구실) ;
  • 이행순 (한국생명공학연구원 식물세포공학연구실) ;
  • 곽상수 (한국생명공학연구원 환경생명공학연구실)
  • Published : 2005.03.01
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Abstract

Chinese leymus (Leymus chinensis Trin.) is a perennial grass that is widely distributed at high pH sodic and arid soil in the northeastern Asia. An efficient regeneration system was established through somatic embryogenesis of mature seeds to understand its high adaptability to harsh environmental conditions on the basis of molecular biology. The calli were efficiently induced (about $70\%$) from mature seeds on MS medium supplemented with $1.5\;\cal{mg/L}$ 2,4-D. Somatic embryos were formed from the surface of embryogenic callus on MS medium supplemented with $2.0\;\cal{mg/L}\;kinetin\;and\;0.5\;\cal{mg/L}$ NAA after 3 weeks of culture. Roots were induced from the shoot when transferred to MS medium without plant growth regulator for 1 week. Plant regeneration rate was $36\%$ and regenerated plantlets were grown to normal mature plants in pot. An efficient plant regeneration system in this study will be useful for molecular breeding of L. chinensis.

양초(Leymus chinensis Trin.)의 성숙종자로부터 캘러스 유도조건 및 식물체 재분화 체계를 확립하였다. 성숙종자로부터 $1.5\;\cal{mg/L}$ 2,4-D가 포함된 MS 배지로부터 배양 6주 후, 캘러스가 높은 빈도 (약 $70\%$)로 유도되었다. 배발생 캘러스는 $2.0\;\cal{mg/L}$ kinetin과 $0.5\;\cal{mg/L}$ NAA가 첨가된 M배지에서 배양 3주 후부터 다양한 단계의 체세포배로 발달하였다. 식물 생장조절제가 포함되지 않은 MS 배지에서 배양4주부터 소식물체로 재분화되었다 (재분화율 $36\%$). 재분화된 소식물체를 1/2 MS 배지에서 1주 동안 배양하여 뿌리가 발달한 완전한 식물체로 성장하였으며 토양에 이식하여 온실에서 정상적인 식물체로 재배할 수 있었다. 본 연구를 통하여 확립한 재분화시스템은 분자육종을 통한 복합환경스트레스 양초의 개발에 유용하게 응용되어질 수 있을 것이다.

Keywords

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