The Mentum Deformity of C. plumosus Following Exposure to Endocrine Disruption Chemicals

내분비교란 물질에 노출된 C. plumosus (장수깔따구)의 하순기절 기형성

  • 이원철 (한양대학교 자연과학대학 생명과학과) ;
  • 곽인실 (한양대학교 자연과학대학 생명과학과)
  • Published : 2005.03.31


Egg masses of C. plumosus were collected from Jungnang Stream in Seoul and reared in an incubator chamber. The larvae of C. plumosus were treated with potential endocrine disruption chemicals (EDCs) such as Diethylhexyl phthalate (DEHP), Bisphenol A (BPA) and Tebufenozide, and the effects of morphological abnormalities were observed. The deformities of the mentum following exposure to EDCs showed the smooth/round tooth, the loss of ${\ge}$1 tooth, reduced median lateral teeth (MLT) and light brown color. The incidence rates of the mentum deformity were associated with chemicals: BPA (73.7 ${\sim}$ 90.9%)>tebufenozide (57.6 ${\sim}$ 78.9%)>DEHP (46.2 ${\sim}$ 85.7%). The deformity type of the mentum showed MIX (MLT+LT, 32 ${\sim}$ 46%)>MLT (25 ${\sim}$ 34%)>LT (lateral teeth, 3 ${\sim}$ 7%). Also, the incidence of MIX type was in the order of BPA (46%)>DEHP (33%) >tebufenozide (32%), and that of MLT type showed DEHP (34%)>tebufenozide (31%)>BPA (25%). As the concentration of Tebufenozide increased, the incidence of light brown mentum was dose dependent. While the incidences of light brown mentum following exposure to BPA and DEHP were not associated with their concentrations.

야외에서 채집한 C. plumosus를 대상으로 잠재적 내 분비계 교란물질인 DEHP, BPA 및 Tebufenozide를 처리물질로 하여 형태적인 교란 효과를 살펴보았다. 약제에 노출된 개체의 하순기절은 부드러워지거나(smooth) 손실(loss)이 가장 많았으며 엷은 갈색을 보였다. 처리 물질에 따른 기형의 정도를 살펴보면, DEHP는 46.2 ${\sim}$ 85.7%, BPA는 73.7 ${\sim}$ 90.9% 그리고 tebufenozide는 57.6 ${\sim}$ 78.9%가 기형을 입어 BPA>tebufenozide>DEHP 순으로 기형이 크게 나타났다. 처리 물질별 기형 부위를 살펴보면, MIX (32 ${\sim}$ 46%)>MLT (25 ${\sim}$ 34%)>LT (3 ${\sim}$ 7%)로 두 부분이 함께 기형을 입는 형태가 가장 많았으며 LT 단독의 기형이 가장 적었다. MIX 형태로 기형이 가장 높은 물질은 BPA (46%)>DEHP (33%)>tebufenozide (32%)이고, MLT에 가장 높은 기형을 보인 것은 DEHP (34%)>tebufenozide (31%)>BPA (25%)로 나타났다. Tebufenozide 처리에 따른 하순기절의 색은 처리 농도가 높아지면 엷은 갈색 하순기절의 발생 빈도가 증가되었다 (27.8 ${\sim}$ 84.2%). 반면, BPA (8.7%)와 DEHP (18.2%)는 $1\;{\mu}g\;L^{-1}$에서 가장 낮은 엷은 갈색의 하순기절의 발생 빈도를 보였으나 $0.3\;{\mu}g\;L^{-1}$처리에서는 매우 높은 엷은 갈색의 하순기절이 다수 발생하였다 (BPA 52.6%; DEHP 30.8%).



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