Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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Toxicogenomics Study on Carbon Tetrachloride-induced Hepatotoxicity in Mice

  • Jeong, Sun-Young (Department of Biopotency/Toxicology Evaluation, University of Science & Technology) ;
  • Lim, Jung-Sun (Korea Institute of Toxicology, Korea Research Institute of Chemical Technology) ;
  • Hwang, Ji-Yoon (Korea Institute of Toxicology, Korea Research Institute of Chemical Technology) ;
  • Park, Han-Jin (Korea Institute of Toxicology, Korea Research Institute of Chemical Technology) ;
  • Cho, Jae-Woo (Korea Institute of Toxicology, Korea Research Institute of Chemical Technology) ;
  • Song, Chang-Woo (Korea Institute of Toxicology, Korea Research Institute of Chemical Technology) ;
  • Kim, Yang-Seok (Bioinformatics Division, ISTECH Inc.) ;
  • Lee, Wan-Seon (Bioinformatics Division, ISTECH Inc.) ;
  • Moon, Jin-Hee (Bioinformatics Division, ISTECH Inc.) ;
  • Han, Sang-Seop (Korea Institute of Toxicology, Korea Research Institute of Chemical Technology) ;
  • Yoon, Seok-Joo (Korea Institute of Toxicology, Korea Research Institute of Chemical Technology)
  • Published : 2005.12.30
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Abstract

Carbon tetrachloride ($CCl_4$) is well known hepatotoxicant. Its overdose cause severe centrilobular hepatic necrosis in human and experimental animals. We administered $CCl_{4}$ at low (0.2 mL/kg p.o.) and high (2 mL/kg p.o.) doses to mice. Mice were sacrificed at 24 h after administration. We evaluated liver toxicity by serum AST and ALT level and by microscopic observation. Using cDNA chip, we conducted gene expression analysis in liver. Mean serum activities of the hepatocellular leakage enzymes, ALT and AST, were significantly increased compare to control, respectively, in the low and high dose groups. H&E evaluation of stained liver sections revealed $CCl_{4}-related$ histopathological findings in mice. Moderate centrilobular hepatocellular necrosis was present in all $CCl_{4}$ treated mice. We found that gene expression pattern was very similar between low and high dose group. However, some stress related genes were differently expressed. These results could be a molecular signature for the degree of liver injury. Our data suggest that the degree of severity could be figure out by gene expression profiling.

Keywords

References

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