Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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Inhibition of Production of Reactive Oxygen Species and Gene Expression Profiles by Cirsii Japonici Herba Extract Treatment in HepG2 Cells

  • Published : 2005.12.30
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Abstract

Cirsii Japonici Herba (CJH) extract has been used for hundreds of years in Asian countries as a treatment for pollutant, radiation, and alcohol-induced liver damage. The reducing effect of CJH on hydrogen peroxide-induced reactive oxygen species (ROS) production, the main cause of cell damage or death, was evaluated using the HepG2 cell line. Cell survival was determined using MTS assay. The viability of cells treated with CJH was not significantly different from oxidative-stressed HepG2 cells. A dose-dependent inhibitory effect by CJH on ROS production was shown in oxidative-stressed cells using the $H_{2}DCFDA$ assay. To identify candidate genes responsible for the anti-oxidative effects of CJH on HepG2 cells, an oligonucleotide microarray analysis was performed. The expressions of five genes were decreased, whereas nineteen genes were up-regulated in CJH plus hydrogen peroxide treated cells, compared to only hydrogen peroxide treated cells. Among them, the expression of 5 genes was decreased in hydrogen peroxide treatment when compared to control. These genes are known to regulate cell survival and progression. On the other hand, it was shown that its main compounds were not a sylimarin or its analogs. The list of differentially expressed genes may provide further insight on the action and mechanism behind the anti-oxidative effects of Cirsii Japonici Herba.

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References

  1. Hunt, J.V., Dean, R.T. & Wolff, S.P. Hydroxyl radical production and autoxidative glycosylation. Glucose autoxidation as the cause of protein damage in the experimental glycation model of diabetes mellitus and ageing. Biochem. J. 256, 205-212 (1988)
  2. Casaril, M., Corso, F. & Corrocher, R. [Free radicals in human pathology]. Recenti. Prog. Med. 82, 39-44 (1991).
  3. Ames, B.N., Shigenaga, M.K. & Hagen, T.M. Oxidants, antioxidants, and the degenerative diseases of aging. Proc. Natl. Acad. Sci. U. S. A. 90, 7915-7922 (1993) https://doi.org/10.1073/pnas.90.17.7915
  4. Shin. M.K. Clinical traditional herbalogy Clinical phytology. Young Lim's Publisher, Seoul, pp 433-434 (1994)
  5. Zhi, F., Kong, L.Y. & Peng, S.X. [Studies on the chemical constituents of Cirsium japonicum DC.]. Yao Xue Xue Bao 38, 442-447 (2003).
  6. Halliwell, B. & Aruoma, O.I. DNA damage by oxygen- derived species. Its mechanism and measurement in mammalian systems. FEBS Lett. 281, 9-19 (1991) https://doi.org/10.1016/0014-5793(91)80347-6
  7. Wagner, H., Horhammer, L. & Munster, R. [On the chemistry of silymarin (silybin), the active principle of the fruits from Silybum marianum (L.) Gaertn. (Carduus marianus L.)]. Arzneimittelforschung 18, 688-696 (1968).
  8. Wellington, K. & Jarvis, B. Silymarin: a review of its clinical properties in the management of hepatic disorders. BioDrugs 15, 465-489 (2001) https://doi.org/10.2165/00063030-200115070-00005
  9. Kireeva, M.L., Mo, F.E., Yang, G.P. & Lau, L.F. Cyr61, a product of a growth factor-inducible immediate- early gene, promotes cell proliferation, migration, and adhesion. Mol. Cell. Biol. 16, 1326-1334 (1996) https://doi.org/10.1128/MCB.16.4.1326
  10. Leu, S.J., Lam, S.C. & Lau, L.F. Pro-angiogenic activities of CYR61 (CCN1) mediated through integrins alphavbeta3 and alpha6beta1 in human umbilical vein endothelial cells. J. Biol. Chem. 277, 46248-46255 (2002) https://doi.org/10.1074/jbc.M209288200
  11. Ho, S.N. The role of NFAT5/TonEBP in establishing an optimal intracellular environment. Arch. Biochem. Biophys. 413, 151-157 (2003) https://doi.org/10.1016/S0003-9861(03)00130-9
  12. Weber, G. et al. Increased signal transduction activity and down-regulation in human cancer cells. Anticancer Res. 16, 3271-3282 (1996)
  13. Pozzan, T., Rizzuto, R., Volpe, P. & Meldolesi, J. Molecular and cellular physiology of intracellular calcium stores. Physiol Rev 74, 595-636 (1994) https://doi.org/10.2466/pr0.1994.74.2.595
  14. Loguercio, C. & Federico, A. Oxidative stress in viral and alcoholic hepatitis. Free Radic. Biol. Med. 34, 1-10 (2003) https://doi.org/10.1016/S0891-5849(02)01167-X
  15. Kaplowitz, N. Mechanisms of liver cell injury. J Hepatol. 32, 39-47 (2000)
  16. Mori, N. & Hirayama, K. Long-term consumption of a methionine-supplemented diet increases iron and lipid peroxide levels in rat liver. J. Nutr. 130, 2349- 2355 (2000) https://doi.org/10.1093/jn/130.9.2349
  17. Loguercio, C. et al. Intravenous load of fructose and fructose 1,6-diphosphate: effects on uricemia in patients with nonalcoholic liver disease. Am J Gastroenterol. 91, 559-564 (1996)
  18. Schlenker, T. et al. Functional interactions between oxidative stress, membrane Na (+) permeability, and cell volume in rat hepatoma cells. Gastroenterol. 118, 395-403 (2000) https://doi.org/10.1016/S0016-5085(00)70222-8
  19. Certa, U., de Saizieu, A. & Mous, J. Hybridization analysis of labeled RNA by oligonucleotide arrays. Methods Mol. Biol. 170, 141-156 (2001)
  20. Lockhart, D.J. et al. Expression monitoring by hybridization to high-density oligonucleotide arrays. Nat Biotechnol. 14, 1675-1680 (1996) https://doi.org/10.1038/nbt1296-1675