Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

Assessment of Factors Influencing Agrobacterium Mediated Transformation in Cucumber (Cucumis sativus L)

  • Sureshkumar P. (Department of Biotechnology, School of Engineering and Technology, Bharathidasan University) ;
  • Selvaraj N. (Department of Botany, Periyar E,V,R College(Autonomous)) ;
  • Ganapathi A. (Department of Biotechnology, Bharathidasan University) ;
  • Kasthurirengan S. (Dept of Biology and Medicinal science, Pai Chai University) ;
  • Vasudevan A. (Department of Forest Resources Development, College of Forest Sciences, Kangwon National University) ;
  • Anbazhagan V. Ramesh (Department of Forest Resources Development, College of Forest Sciences, Kangwon National University)
  • 발행 : 2005.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Five day old cotyledon explants of Cucumber (Cucumis sativus L) cv Poinsett 76 were cocultivated with two Agrobacterium strains (EHA105 and LBA 4404) each carrying GUS as the reporter gene and npt-II as the selection marker gene in the T-DNA region of the vector. Transformed shoots were selected at 150 mg/L kanamycin. A two day cocultivation coupled with $20\;{\mu}M$ acetosyringone increased the frequency (8.2 and 15.4 shoots) of GUS expression in the shoots of transformed plant. Among the two Agrobacterium strains, EHA 105 performed better than LBA 4404 in bringing two-fold increase in transformation efficiency (14%) than LBA 4404 (7.4%). PCR analysis was done to confirm the integration of T-DNA into cucumber genome.

키워드

참고문헌

  1. Burza W, Plader S (1995) Direct plant regeneration from leaf explant in Cucumber (Cucumis sativus L.) is free of stable genetic variation. Plant Breed 12: 341-345
  2. Cao X, Liu Q, Rowland LJ, Hammerschlag FA (1998) GUS expression in blueberry (Vaccium spp.) factors influencing Agrobacterium-mediated gene transfer efficiency. Plant Cell Rep 18: 266-270 https://doi.org/10.1007/s002990050569
  3. Cervera M, Pina JA, Juarez J, Navarro L, Pena L (1998) Agrobacterium-mediated transformation of Citrange : factors affecting transformation and regeneration. Plant Cell Rep 18: 271-278 https://doi.org/10.1007/s002990050570
  4. Chee PP. (1990) Transformation of Cucumis sativus tissue by Agrobacterium tumefaciens and the regulations of transformed plants. Plant Cell Rep 9: 245-248
  5. Chee PP, Slightom, JL (1991) Transfer and expression of Cucumber Mosaic Virus Coat protein gene in the genome of Cucumis sativus. J. Amer Soc Sci Hortic 116: 1098-1102
  6. Chilton MD, Currier TC, Farrand SK, Bendich AJ, Gordon MP, Nester EW (1974) Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proct Natl Acad Sci USA 71 :3672-2.676 https://doi.org/10.1073/pnas.71.9.3672
  7. De Bondt A (1994) Agrobacterium mediated transformation of apple (Malus x domestica Borkh): An Assessment of factors affecting gene transfer efficiency during early transformation steps. Plant Cell Rep13: 587-593 https://doi.org/10.1007/BF00234517
  8. Esquinas-Alcazer JT, Gulick PJ (1983) Genetic resources of Cucurbitaceae. IBPGR
  9. Gaba V, Feldmesser E, Amit Gal-on, Antigus KY (1995) Genetic transformation of a recalcitrant melon (Cucumis meta L.) variety. In: Lester G, Dunlap et al. (eds), Cucurbitaceae' 94. Gateway printing, USA, pp 188-190
  10. Ganapathi A, Perl-Treves R (2000) Agrobacterium mediated transformation in Cucumis sativus L. via direct organogenesis. Acta Horticult 510: 405-407
  11. Gomez KA, Gomez KA (1976) Statistical Procedure for Agricultural research, John Wiley and Sons, New Delhi
  12. Hood E.E, Gelvin S.B, Melchers, Hoekema A (1993) New Agrobacterium vectors for plant transformation. Transgenic Research 2:208-218 https://doi.org/10.1007/BF01977351
  13. Hood E.E, Helmer G.C, Fraley RT, Chilton M.D (1986) The hypovirulence of Agrobacterium tumefaciens A281 is encoded in the region of PtiB0542 outside the T-DNA. J Bactriol 168:1291-1301 https://doi.org/10.1128/jb.168.3.1291-1301.1986
  14. Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusion:$\beta$-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901-390-7
  15. Mohinuddin AKM, Harikrishna K, Chowdhury MKU, Abdillah ZC, Napis S (2000) Influence of Acetosyringone on Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.). Plant Tissue Cult 10: 167-173
  16. Mourgues F, Chevreau E, Lambert C, De Bondt R.(1996) Efficient Agrobacterium mediated transformation and recovery of transgenic plants from pear (Pyrus communis L.). Plant Cell Rep 15: 809-814 https://doi.org/10.1007/BF00233145
  17. Muraskige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  18. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321-4325 https://doi.org/10.1093/nar/8.19.4321
  19. Nagata T, Okada K, Kawazu T, Takebe I (1987) Cauliflower mosaic virus 35S promoter directs S phase specific expression in plant cells. Mol Gen Genet 207:242-244 https://doi.org/10.1007/BF00331584
  20. Nishibayashi S, Kaneko H, Hayakawa T (1996) Transformation of Cucumber (Cucumis sativus L.) plants using Agrobacterium tumefaciens and regeneration from hypocotyl explants. Plant Cell Rep 15: 809-814 https://doi.org/10.1007/BF00233145
  21. Pichon M, Journet EP, Dedieu A, Billy F de, Truchet G, Barker, DG (1992) Rhizobium melitoli elicitis transient expression of the early nodulin gene Enod12 in the differentiating toot epidermis of transgenic alfafa. Plant Cell Rep 4: 1199-1211 https://doi.org/10.1105/tpc.4.10.1199
  22. Plader W, Burza W, Rusinowski Z (1998) The relationship between the regeneration system and genetic variability in the cucumber (Cucumis sativus L.). Euphytica 103: 9-15 https://doi.org/10.1023/A:1018359726626
  23. Raharjo SHT, Hernandey MO, Zhang YY, Punja, ZK (1996) Transformation of pickling Cucumber with chitinase - encoding genes using Agrobacterium tumefaciens. Plant Cell Rep 15: 591-596 https://doi.org/10.1007/BF00232459
  24. Sarmento GG, Alpert K, Tang FA, Punja ZK (1992) Factors influencing Agrobacterium tumefaciens-mediated transformation. Plant Cell Tiss Org Cult 31: 185-193
  25. Sharma AK, Mohanty A, Singh Y, Tyagi AK (1999) Transgenic plants for the production of edible vaccines and antibodies for immunotherapy. Curr. Sci 77: 524-529
  26. Suresh kumar, P (2002) In vitro studies and Agrobacterium mediated gene transformation in Cucumis sativus . Ph.D Thesis
  27. Tabei Y, Nishio T, Kurihara K, Kanuo T(1994). Selection of transformed callus in a liquid medium and regeneration of transgenic plants in cucumber (Cucumis sativus L.). Breed Sci 44:47-51
  28. Tabei Y, Kitade S, Nisihizawa Y, Kikuchi N, Kayano T, Hibi, T, Akutsu K (1998) Transgenic cucumber plants harboring a rice chitinase gene exhibit enhanced resistance to gray mold (Botrytis cinerea). Plant Cell Rep 17: 159-164 https://doi.org/10.1007/s002990050371
  29. Trulson, AJ, Shahin EA (1986). In vitro plant regeneration in the genus Cucumis. Plant Sci 47: 35-43 https://doi.org/10.1016/0168-9452(86)90008-7
  30. Valles MP, Lasa JM (1994). Agrobacterium-mediated transformation of commercial melon (Cucumis melo L, cv. Amarillo Oro) Plant Cell Rep 13: 145-148
  31. Van Wordragen MF, Dons HJM(1992). Agrobacterium tumefaciens mediated transformation of recalcirant crops. Plant Mol Biol Rep 10:12-36 https://doi.org/10.1007/BF02669262
  32. Vasudevan A, Ganapathi A, Selvaraj N, Vengadesan G (2002). Factors influencing GUS expression in Cucumber (Cucumis sativus Linn.) Indian Journal of Biotechnology Vol 1, 344-349