Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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24-Epibrassinolide Modulate Cellular and Organogenic Response of Explants of Brassica Species, in vitro Culture

  • Rocha Andrea da S.R. (Centro de Biotecnologia e, UFPel. Campus Universitario) ;
  • Coutinho Camila M. (Centro de Biotecnologia e, UFPel. Campus Universitario) ;
  • Braga Eugenia J.B. (Departamento de Botanica da Universidade Federal de Pelotas, UFPel. Campus Universitario) ;
  • Peters Jose A. (Departamento de Botanica da Universidade Federal de Pelotas, UFPel. Campus Universitario) ;
  • Binsfeld Pedro Canisio (Departamento de Botanica da Universidade Federal de Pelotas, UFPel. Campus Universitario)
  • Published : 2005.09.01
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Abstract

Brassinosteroids are steroidal plant hormones and are known to modulate physiological and cellular response in a wide range of plant species. Considerable insights has been achieved of the physiological role of brassinosteroid in Brassica species in the past few years, but their effect on direct organogenesis has not been extensively studied. In this sense, under optimal basal media and growth conditions we tested the cellular and organogenic response of 24-epibrassinolide (EBL) in a variable concentration (0.1 to $5.0\;{\mu}M$) and Zeatin (Z) (1.0 to $100\;{\mu}M$) and their synergic effect on hypocotyl explants of cauliflower and broccoli. The isolated EBL accelerated cell elongation and promotes direct organogenesis. One micromolar EBL + $10\;{\mu}M$ of Z was the most efficient combination for cell elongation, cell differentiation as well as for organogenesis. A suppressing effect on root induction was confirmed for all the tested hormone levels. The general results indicate a synergic effect of EBL-Z and EBL potentates Zeatin activity, at least in certain tissues. Besides de genetic factors, we can speculate that the natural hormone concentration in the explants might affect the responses by application of exogenous growth regulators. Experiments with new plant growth regulators, like brassinolide, are important aiming to maximize or accelerate plant regeneration for in vitro multiplication or for genetic transformation.

Keywords

References

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