Journal of Plant Biotechnology

  • 제32권2호
  • /
  • Pages.129-134
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  • 2005
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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두릅나무 '자오'의 체세포배 유도와 식물체 형성에 미치는 생장조절제 및 삼투압제 효과

Effect of Growth Regulators and Osmoticums on Somatic Embryogenesis and Plants Regeneration in Aralia elata Cultivar 'Zaoh'

  • 김지아 (국립산림과학원 생물공학과) ;
  • 문흥규 (국립산림과학원 생물공학과) ;
  • 김용욱 (국립산림과학원 생물공학과)
  • Kim Ji-Ah (Biotechnology Division., Korea Forest Research Institute) ;
  • Moon Heung-Kyu (Biotechnology Division., Korea Forest Research Institute) ;
  • Kim Yong-Wook (Biotechnology Division., Korea Forest Research Institute)
  • 발행 : 2005.06.01
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초록

체세포배 유도를 통해 두릅나무 역병 저항성 개체인 '자오'의 효율적인 재분화가 가능하였다. 절편에 따라 배발생 캘러스에 요구되는 생장조절제의 종류가 다르게 나타났다. 잎의 경우 MS 배지에 2,4-D와 TDZ, L-glutamine이 처리된 배지에서 캘러스와 체세포배의 유도가 양호하였으며, 엽병과 뿌리는 2,4-D 단독처리 시 가장 좋은 결과를 얻을 수 있었다. 적정 배양조건하에서 배발생캘러스 유도율은 잎, 엽병, 뿌리로부터 각각 75%, 67% 및 83%를 나타냈다. 삼투압제 처리에 따른 체세포배의 발아율은 sucrose, glucose, maltose모두 90% 이상의 발아율을 보였으나, 식물체 형성은 2% sucrose에서 72%로 가장 좋았다. 어린 식물체는 생장조절제를 첨가하지 않은 1/2MS 배지에서 정상 식물체로 생장하였고, 인공상토에 이식하여 95% 이상 순화하였다. 본 실험결과는 두릅나무 역병 저항성 개체 '자오'의 체세포 배발생을 통한 기내 대량증식이 가능함을 보여주었다.

Effective micropropagation system via somatic embryognesis was established for a Phytophthora resistant Aralia elata cultivar. Different kinds of growth regulators were needed to induce embryogenic callus with different explant sources. When leaf explants were used, a combination of 2,4-D, TDZ and L-glutamine was needed, whereas when petiole and root explants needed only 1.0 mg/L 2,4-D. Embryogenic callus induction rate under the optimum culture condition was 75.0%, 67.0% and 83.0% from leaf, petiole and root segment, respectively. Somatic embryo germination and plantlet conversion rate appeared to be influenced greatly by various osmoticums. More than 90% of embryos germinated when treated with sucrose, glucose and maltose. However, the highest conversion rate (72%) was recorded on medium with 2% sucrose only. The converted plantlets grew normally on 1/2MS basal medium, were acclimatized on artificial soil mixture and survived more than 95% in the greenhouse condition. The results suggest that the species can be clonally propagated through in vitro culture system via somatic embryogenesis.

키워드

참고문헌

  1. Amemiya K, Fujiki T, Hyuga S (1990) Mass propagation by tissue culture in Japanese angelica tree (Aralia elata). Ann. Rep. Vamanasi Agri Exp Stn (Japan) 5: 11-22
  2. Amemiya K, Mochizuki T (2002) Somatic embryo formation and plant regeneration in 'Zaoh' line No.2 of Japanese Angelica tree (Aralia elata Seem.). Plant Biotech 19: 383-388 https://doi.org/10.5511/plantbiotechnology.19.383
  3. Biahoua A, Bonneau, L (1999) Control of in vitro somatic embryogenesis of the spindle tree (Euonymus europaeus L.) by the sugar type and the osmotic potential of the culture medium. Plant Cell Rep 19: 185-190 https://doi.org/10.1007/s002990050731
  4. Fuentes SRL, Calheiros MBP, Manetti-Filho J, Vieira LGE (2000) The effects of silver nitrate and different carbohydrate sources on somatic embryogenesis in Correa canephora. Plant Cell Tiss Org Cult 60: 5-13 https://doi.org/10.1023/A:1006474324652
  5. Hernandez I, Celestino C, Alegre J, Toribio M (2003) Vegetative propagation of Quercus suber L. by somatic embryogenesis: II. Plant regeneration from selected cork oak trees. Plant Cell Rep 21: 765-770
  6. Iraqi D, Tremblay FM (2001) The role of sucrose during maturation of black spruce (Picea mariana) and white spruce (Picea glauca) somatic embryos. Physiol Plant 111: 381-388 https://doi.org/10.1034/j.1399-3054.2001.1110316.x
  7. Jhang HH, Park CH, Cho DH, Shin VB (1993) Callus induction and plant regeneration from leaf tissue culture of Aralia elata S. Kor J Crop Sci 38: 366-370
  8. Jhang HH, Park CH, Lee VS, Shin VB (1994) Somatic embryogenesis and plant regeneration in suspension cultures of Aralia elata Seem. Kor J Plant Tiss Cult 21: 167-171
  9. Kaimori N (1986) Mass propagation of Japanese angelica tree (Aralia elata) through biotechnology. Agri Hort 61: 75-77
  10. Karhu ST (1997) Sugar use in relation to shoot induction by sorbitol and cytokinin in apple. J Am Soc Hort Sci 122: 476-480
  11. Ladyman JAR, Girard B (1992) Cucumber somatic embryo development on various gelling agents and carbohydrate sources. HortSci 27: 164 - 165
  12. Lee WS, Choi EG, Kim JH (2004) Mass propagation of somatic embryos and plantlets of Aralia elata through biorector culture. Kor J Plant Biotech 31: 219-223 https://doi.org/10.5010/JPB.2004.31.3.219
  13. Lou H, Kako S (1995) Role of high sugar concentration in inducing somatic embryogenesis from cucumber cotyledons. Sci Hort 64: 11-20 https://doi.org/10.1016/0304-4238(95)00833-8
  14. Mamiya K, Sakamoto Y (2000) Effect of concentration and strength of basal medium on conversion of somatic embryos in Asparagus offcinalis L. Sci Hort 84: 15-26 https://doi.org/10.1016/S0304-4238(99)00098-9
  15. Moon HK, Youn V (1999) Somatic embryogenesis from winter buds of 10-year-old Aralia elata. In : SM Jain, PK Gupta, RJ Newton (eds.), Somatic Embryogenesis in Woody Plants Vol 5, Kluwer Aca Pub, pp 129-134
  16. Moon HK, Oh KE, Son SH (1999) Factors influencing somatic embryo induction and plant regeneration in Aralia elata Seem. Kor J Plant Tiss Cult 26: 275-280
  17. Moon HK, Hong YP, Kim YW, Lee JS (2001) Genotype effect on somatic embryogenesis and plant regeneration of 15 Aralia elata. Kor J Plant Tiss Cult 28: 129-134
  18. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 437-479 https://doi.org/10.1111/j.1399-3054.1962.tb08047.x
  19. Park CH, Lee YS, Jhang HH, Kim NS, Shin, YB (1994) Effect of media and plant growth regulator on germination of somatic embryos of Aralia elata SEEM. Kor J Med Crop Sci 2: 241-245
  20. Ramarosandratana A, Harvengt L, Bouvet A, Galvayrac R, Paques M (2001) Effect of carbohydrate source, polyethylene glycol and gellan gum concentration on embryonalsuspensor mass proliferation and maturation of maritime pine somatic embryos. In Vitro Cell Dev Bioi-Plant 37: 29-34 https://doi.org/10.1007/s11627-001-0006-1
  21. Rodriguez APM, Vendrame WA (2003) Micropropagation of tropical woody species. In : SM Jain and K Ishii (eds.), Micropropagation of Woody Trees and Fruits, Kluwer Academic Pub, pp 153-179
  22. Tachikawa Y, Saitou T, Kamada H, Harada H (1998) Changes in protein pattern during stress induction of carrot (Daucus carota L.) somatic embryogenesis. Plant Biotechnol 15: 17-22 https://doi.org/10.5511/plantbiotechnology.15.17
  23. Tomatsu M, Ohnishi-Kameyama M, Shibamoto N (2003) Aralin, a new cytotoxic protein from Aralia elata, inducing apoptosis in human cancer cells. Cancer Lett 199: 19-25 https://doi.org/10.1016/S0304-3835(03)00348-3
  24. Yang Z, Zhang L, Diao F, Huang M, Wu N (2004) Sucrose regulates elongation of carrot somatic embryo radicles as a signal molecule. Plant Mol Biol 54: 441-459 https://doi.org/10.1023/B:PLAN.0000036375.40006.d3

피인용 문헌

  1. A review of forest trees micropropagation and its current status in Korea vol.37, pp.4, 2010, https://doi.org/10.5010/JPB.2010.37.4.343
  2. Somatic embryogenesis and plant regeneration from immature seeds of Magnolia obovata Thunberg vol.1, pp.4, 2007, https://doi.org/10.1007/s11816-007-0037-0