Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of Growth Regulators and Osmoticums on Somatic Embryogenesis and Plants Regeneration in Aralia elata Cultivar 'Zaoh'

두릅나무 '자오'의 체세포배 유도와 식물체 형성에 미치는 생장조절제 및 삼투압제 효과

  • Kim Ji-Ah (Biotechnology Division., Korea Forest Research Institute) ;
  • Moon Heung-Kyu (Biotechnology Division., Korea Forest Research Institute) ;
  • Kim Yong-Wook (Biotechnology Division., Korea Forest Research Institute)
  • 김지아 (국립산림과학원 생물공학과) ;
  • 문흥규 (국립산림과학원 생물공학과) ;
  • 김용욱 (국립산림과학원 생물공학과)
  • Published : 2005.06.01
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Abstract

Effective micropropagation system via somatic embryognesis was established for a Phytophthora resistant Aralia elata cultivar. Different kinds of growth regulators were needed to induce embryogenic callus with different explant sources. When leaf explants were used, a combination of 2,4-D, TDZ and L-glutamine was needed, whereas when petiole and root explants needed only 1.0 mg/L 2,4-D. Embryogenic callus induction rate under the optimum culture condition was 75.0%, 67.0% and 83.0% from leaf, petiole and root segment, respectively. Somatic embryo germination and plantlet conversion rate appeared to be influenced greatly by various osmoticums. More than 90% of embryos germinated when treated with sucrose, glucose and maltose. However, the highest conversion rate (72%) was recorded on medium with 2% sucrose only. The converted plantlets grew normally on 1/2MS basal medium, were acclimatized on artificial soil mixture and survived more than 95% in the greenhouse condition. The results suggest that the species can be clonally propagated through in vitro culture system via somatic embryogenesis.

체세포배 유도를 통해 두릅나무 역병 저항성 개체인 '자오'의 효율적인 재분화가 가능하였다. 절편에 따라 배발생 캘러스에 요구되는 생장조절제의 종류가 다르게 나타났다. 잎의 경우 MS 배지에 2,4-D와 TDZ, L-glutamine이 처리된 배지에서 캘러스와 체세포배의 유도가 양호하였으며, 엽병과 뿌리는 2,4-D 단독처리 시 가장 좋은 결과를 얻을 수 있었다. 적정 배양조건하에서 배발생캘러스 유도율은 잎, 엽병, 뿌리로부터 각각 75%, 67% 및 83%를 나타냈다. 삼투압제 처리에 따른 체세포배의 발아율은 sucrose, glucose, maltose모두 90% 이상의 발아율을 보였으나, 식물체 형성은 2% sucrose에서 72%로 가장 좋았다. 어린 식물체는 생장조절제를 첨가하지 않은 1/2MS 배지에서 정상 식물체로 생장하였고, 인공상토에 이식하여 95% 이상 순화하였다. 본 실험결과는 두릅나무 역병 저항성 개체 '자오'의 체세포 배발생을 통한 기내 대량증식이 가능함을 보여주었다.

Keywords

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