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Optimization of Treatment Concentration and Screening of Exogenous Plant Growth Regulators for Improvement of Salidroside Yield in Rhodiola sachalinensis A. Bor Cell Suspension Cultures

참돌꽃 (Rhodiola sachalinensis A. Bor) 현탁세포배양에서 Salidroside 생산을 위한 외재 호르몬의 영향 및 처리 농도의 최적화

  • Choi Hye-Jin (Dept. of Biology, Chonnam National University) ;
  • Kim Su-Jeong (Kumho Life & Enviromental science Lab, Korea Kumho Petrochemical Co., Ltd) ;
  • Hwang Baik (Dept. of Biology, Chonnam National University) ;
  • Ahn Jun-Cheul (Dept. of Life Science, Seonam University, SM-bio Co.)
  • 최혜진 (전남대학교 생물학과) ;
  • 김수정 (금호석유화학 연구소) ;
  • 황백 (전남대학교 생물학과) ;
  • 안준철 (서남대학교 생명과학과, (주)에스엠바이오)
  • Published : 2005.06.01

Abstract

To enhance salidroside productivity from a cell suspension cultures of Rhodiola sachalinensis, various combinations of auxin (NAA) and cytokinins (BA, Kinetin) concentration and addition of $GA_3$, TDZ, zeatin, spermine and spermidine at a hormone combination to be established were examined. NAA/BA combination is superior to NAA/kinetin combination in biomass and salidroside content. Maximum salidroside production ($64.6{\pm}8.9\;mg/L$ medium) was obtained from $2B_5$ medium with 1 mg/L NAA and 5 mg/L BA. The adding of $GA_3$ ($0.01\;mg/L{\sim}5\;mg/L$) was beneficial for salidroside accumulation and the highest productivity of salidroside, $90.3{\pm}8.34\;mg/L$, was obtained from $2B_5$ medium supplemented with 1 mg/L NAA, 5 mg/L BA and 0.1 mg/L $GA_3$. On TDZ, zeatin, spermine and spermidine, expectant results were not obtained except a little affirmative effect of spermidine ($69{\pm}2.88$ at 1 mg/L).

참돌꽃 (Rhodiola sachalinensis A. Bor) 현탁배양에서 salidroside 생산성을 개선하고자 auxin인 NAA와 cytokinin인 BA와 kinetin, 그 외에 $GA_3$, TDZ, zeatin, spermine과spermidine을 조합하여 조사하였다. NAA/BA 조합은 NAA/kinetin조합과 비교했을 때 생장과 salidroside 함량이 증가하였다. Salidroside 함량의 증가에 의한 생산성 ($64.6{\pm}8.9$ mg/L medium)은 1 mg/L. NAA와 5 mg/L BA가 첨가된 $2B_5$ 배지에서 높게 나타났으며, 최대 salidroside 의 생산성은 1 mg/L NAA, 5 mg/L BA와 1 mg/L $GA_3$이 첨가된$2B_5$ 배지에서 $90.3{\pm}8.34$ mg/L로 나타났다. 그 밖의 TDZ. zeatin, spermine과 spermidine 처리에서는 생산성에 있어서 증가를 보이지 않았으며, spermidine의 경우에 약간의 유의적인 생산성 증가를 보였다.

Keywords

References

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