Anti Angiogenic Effects of Isorhamnetin Isolated from Persicaria thunbergii

  • Lee Hyo-Jung (Department of Oncology, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim Kwan-Hyun (Department of Oncology, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Baek Nam-In (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Kim Dae-Keun (Department of Pharmacy, WooSuk University) ;
  • Yang Deok-Chun (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Kim Sung-Hoon (Department of Oncology, Graduate School of East-West Medical Science, Kyung Hee University)
  • Published : 2005.12.01

Abstract

Persicaria thunbergii has been utilized for the treatment of cancer as a folk medicine. We examined the effect of isorhamnetin, a flavonoid isolated from Persicaria thunbergii, on angiogenesis in vitro and in vivo. Basic fibroblast growth factor (bFGF) is a potent angiogenic factor found in various tumors. In this study, we found that the isorhamnetin decreased bFGF-induced human umbilical vein endothelial cells (HUVECs) proliferation and migration in a concentration-dependent manner (5, 10 and $20\;{\mu}M$) whereas, it did not inhibit bFGF-induced capillary-like formation of HUVECs. The chicken chorioallantoic membrane assay revealed that addition of isorhamnetin (10, 20 and $40\;{\mu}M$) displayed an antiangiogenic effect in vivo. These results suggest that the isorhamnetin inhibits the proliferation and migration of endothelial cells induced by bFGF, which may explain its anti-angiogenic properties.

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