Plant Resources

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Isolation and Characterization of Cinnamoyl CoA Reductase Gene from Codonopsis lanceolata

  • Kim Ha-Na (Dept. of Oriental Medicine Material and Processing, Kyung Hee University) ;
  • In Jun-Gyo (Biopia Co., Ltd.) ;
  • Lee Eun-Kyung (Biopia Co., Ltd.) ;
  • Yoon Jae-Ho (College of Agriculture and Life Sciences, Sunchun National University) ;
  • Lee Mee-Sook (College of Food and Nutrition, Hannam University) ;
  • Bae Hyeun-Jong (Department of Forest Products & Technology, Chonnam National University) ;
  • Yang Deok-Chun (Dept. of Oriental Medicine Material and Processing, Kyung Hee University)
  • Published : 2005.12.01
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Abstract

A cinnamoyl CoA reductase (CCR) cDNA (ClCCR) was isolated from tabroot mRNAs of Codonopsis lanceolata by cDNA library construction, and its expression was investigated in relation to abiotic stresses. The ClCCR is 1008 bp in length with an open reading frame (ORF) of 336 amino acids. The deduced amino acid sequence was showed high similarity with cinnamoyl-CoA reductases of P. tremuloides (AAF43141) 87%, F.${\times}$aranassa (AAP46143) 83%, L. album (CAD29427) 80%, E. gunnii (CAA66063) 72%, S. tuberosum (AAN71761) 83%. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was revealed that the ClCCR expression was regulated by abiotic stresses.

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