Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Apoptotic Effects and Mechanism Study of Scopoletin in HepG2 Cells

Scopoletin의 간암세포에 대한 고사 유도 효과 및 기전 연구

  • Kwon Kang-Beom (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Kim Eun-Kyung (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Park Sung-Joo (Department of herbology, School of Oriental Medicine, Wonkwang University) ;
  • Song Ho-Joon (Department of herbology, School of Oriental Medicine, Wonkwang University) ;
  • Lee Young-Rae (Department of Biochemistry, Institute for Healthcare Technology Development, Medical School, Chonbuk National University) ;
  • Park Byung-Hyun (Department of Biochemistry, Institute for Healthcare Technology Development, Medical School, Chonbuk National University) ;
  • Park Jin-Woo (Department of Biochemistry, Institute for Healthcare Technology Development, Medical School, Chonbuk National University) ;
  • Ryu Do-Gon (Department of Physiology, School of Oriental Medicine, Wonkwang University)
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 김은경 (원광대학교 한의과대학 생리학교실) ;
  • 박성주 (원광대학교 한의과대학 본초학교실) ;
  • 송호준 (원광대학교 한의과대학 본초학교실) ;
  • 이영래 (전북대학교 의과대학 생화학교실, 전북대학교 헬스케어기술개발사업단) ;
  • 박병현 (전북대학교 의과대학 생화학교실, 전북대학교 헬스케어기술개발사업단) ;
  • 박진우 (전북대학교 의과대학 생화학교실, 전북대학교 헬스케어기술개발사업단) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2005.12.01
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Abstract

Scopoletin (6-methoxy-7-hydrorycournarin) is a phenolic coumarin and a member of the phytoalexins. In this study we investigated whether scopoletin causes apoptosis in human hepatoma HepG2 cells and, if so, by what mechanisms. We report that scopoletin induced apoptosis as confirmed by a chromatin condensation. The signal cascade acivated by scopoletin included the activation of caspase-3 as evidenced by increased pretense activity. Activation of caspase-3 resulted in the cleavage of 116 kDa poly(ADP-ribose) polymerase (PARP) to 85 kDa cleavage product in a dose-dependent fashion. Also, scopoletin-induced apoptotic mechanism of HepG2 cells involved the generation of hydrogen peroxide. Taken together, these results suggest that scopgletin induces hydrogen peroxide generation, which, in turn, causes activation of caspase-3, degradation of PARP, and eventually leads to apoptotic cell death in HepG2 cells.

Keywords

References

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