Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Immunohistochemical Study of Wild Ginseng on Benzopyrene Induced $TNF-{\alpha}$ and COX-2 Expression in Rats

장뇌산삼이 Benzopyrene으로 유도된 간조직의 $TNF-{\alpha}$와 COX-2의 면역조직학적 분포에 미치는 영향

  • Ahn Sang-Hyun (Department of Anatomy, The College of Oriental Medicine. Dongguk University) ;
  • Jo Sung-Jun (Oriental Medicine-Bio R&D Center, Dongguk University) ;
  • Yoon Chang-Hwan (Oriental Medicine-Bio R&D Center, Dongguk University) ;
  • Cho Min-Kyung (Department of Pharmacology, The College of Oriental Medicine. Dongguk University) ;
  • Kim Jin-Taek (Department of Anatomy, The College of Oriental Medicine. Dongguk University) ;
  • Shin Heung-Muk (Department of Physiology, The College of Oriental Medicine. Dongguk University)
  • 안상현 (동국대학교 한의과대학 해부학교실) ;
  • 조성준 (동국대학교 한의바이오혁신사업단) ;
  • 윤창환 (동국대학교 한의바이오혁신사업단) ;
  • 조민경 (동국대학교 한의과대학 약리학교실) ;
  • 김진택 (동국대학교 한의과대학 해부학교실) ;
  • 신흥묵 (동국대학교 한의과대학 생리학교실)
  • Published : 2005.12.01
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Abstract

Polycyclic aromatic hydrocarbon (PAH), such as benzo(a)pyrene (B(a)P), are toxic environmental contaminants known to enhance oxidative stress, production of pro-inflammatory and inflammatory cytokines. The present study was designed in order to determine whether wild ginseng (Panax ginseng C. A. Meyer) protect PAH-induced oxidative stress and inflammation. B(a)P (0.5 mg/kg, i.p.) treatment increased the distribution of immunoreactive cells for tumor necrosis factor $(TNF)-\alpha$ and cyclooxygenase (COX)-2 in peri-portal triad region and immunoreaction was shown in the cytoplasm of macrophage. Pre-treatment with wild ginseng significantly decreased immune responses in the rats treated with B(a)p. The rats given 50 mg/kg/day for 4 weeks before B(a)P treatment had 1.39-fold and 1.5-fold inhibition of $TNF-\alpha$ and COX-2 positive reaction, respectively. Wild ginseng extract alone had no effect on the distributional changes. The SOD activity as scavenger enzymes after wild ginseng administration dose-dependantly increased compared with butylated hydroxytoluene, a general radical scavenger. These data likely indicate that wild ginseng extract may act as inflammatory regulator in conjunction with inhibition of oxidant dependent metabolic activation in environmental contaminants-induced hepatic inflammation.

Keywords

References

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