Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Antioxidative Activity and Irritation Test of a Complex Herbal Bath Extracted from Korean Traditional Plants

  • Chang Zhiqiang (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim Young-Hoan (Kyungpook Veterinary Service Laboratory) ;
  • Song Jae-Chan (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim Nam-Woo (Department of Herbal Biotechnology, Daegu Haany University) ;
  • Lee In-Seon (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee Sam-Pin (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Kim Jong-Choon (College of Veterinary Medicine, Chonnam National University) ;
  • Park Seung-Chun (College of Veterinary Medicine, Kyungpook National University)
  • Published : 2005.03.01
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Abstract

The purpose of this study is to evaluate the free radical scavenging ability and xanthine oxidase inhibitory activity of a complex herbal bath consisted of Artemisiae argyi folium, Angelicae sinensis radix, Ligustici wallichii radix and Angelicae tuhuo radix, and its potential irritation response were also tested for safety use in the rabbits. For antioxidative activity, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the complex herbal bath were examined at five different concentrations (0, 250, 500, 1000 and 2000 ㎍/ml). The concentration of the complex herbal bath required for scavenging DPPH free radical by 50% was 897.2 ㎍/ml. In the inhibition of xanthine oxidase (XO) activity, the concentration of the complex herbal bath required for 50% of inhibition was 221.4 ㎍/ml. In the skin irritation study in rabbit, all animals survived for the duration of the study and the examined skin exhibited no edema, erythema, and eschar formation. In the ocular irritation study in rabbit, after application of the sample to eyes, all of the eyes were normal. In summary, the complex herbal bath has potent antioxidant effects against the DPPH radical and XO and was considered to be a non-irritation bath for safety use.

Keywords

References

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