The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Antioxidative and Antimutagenic Effects of Ganoderma lucidum Krast Extracts

영지버섯(Ganoderma lucidum Krast) 추출물의 항산화 및 항돌연변이 효과

  • Oh Se-In (Dept. of Food and Nutrition, Seoil College) ;
  • Lee Mee-Sook (Dept. of Food and Nutrition, Hannam University)
  • Published : 2005.03.01
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Abstract

The antioxidative effect, antimutagenic capacity and inhibitory effect of cancer cell proliferation in ethanol extracts of Ganoderma lucidum were studied for suggestion of prevention and dietetic treatment of chronic diseases and development of antioxidative, antimutagenic and anticancer functional food by employing biological and biochemical assay. The IC/sub 50/ of MDA with BSA conjugation reaction, lipid peroxidation and scavenging effect on DPPH radical in ethanol extracts of Ganoderma lucidum showed 1026.21 mg/mL, 0.152 mg/mL and 0.412 mg/mL, respectively. So, the most effective antioxidative capacity in ethanol extracts of Ganoderma lucidum was the lipid peroxidation, among the method used this study. The indirect and direct antimutagenic effects of ethanol extracts of Ganoderma lucidum were examined by Ames test using Salmonella typimurium TA98 and TA100. The inhibition rates on indirect mutagenicity mediated by 2-Anthramine showed 100% in the Salmonella typimurium TA98 and 98.74% in the Salmonella typimurium TA100 and the direct mutagenicity mediated by sodium azide in Salmonella typimurium TA100 was 82.96%. But, the inhibitory effect on indirect mutagenicity mediated by 2-Nitrofluorene in Salmonella typimurium TA98 was low(7.81%). The inhibitory Effect of Ganoderma lucidum ethanol extracts on cell proliferation in Hela and MCF-7 by MTT test were 74.36% in HeLa and 73.90% in MCF-7 at the 0.50 mg/assay concentration and IC/sub 50/ were 0.163 mg/mL and 0.196 mg/mL respectively. From this result, it is suggested that Ganoderma lucidum is believed to have a possible antioxidative, antimutagenic and anticancer capacities.

Keywords

References

  1. Farag, RS, Badei, AZMA and Baroty, GSAE. Influence of thyme and clove essential oils in cotton seed oil oxidation. JAOCS 66:800-804. 1989 https://doi.org/10.1007/BF02653671
  2. Lrson, RA. The antioxidants of higher plants. Phytochemistry 27:969-978. 1988 https://doi.org/10.1016/0031-9422(88)80254-1
  3. Colditz, GA, Branch, LG, Lipnick, RJ, Willett, WC, Rosner, B, Posner, BM and Hennekens, CH. Increased green and yellow vegetable intake and lowered cancer deaths in an elderly population. Am. J. Clin. Nutr. 41 :32-36. 1985
  4. Kim, JO, Kim, YS, Lee, JH, Kim, MN, Rhee, SH, Moon, SH and Park, KY. Antimutagenic effect of the major volatile compounds identified from Mugwort (Artemisia asictica Nakai) leaves. J. Korean Soc. Food Nutr. 21(3):308-313. 1992
  5. Tada, M, Matsumoto, R, Yamaguchi, H and Chiba, K. Novel antioxidants isolated from Perilla frutescnes Britton var. crisp(Thunb.). Biosci. Biotech. Biochem. 60(8):1093-1095. 1996 https://doi.org/10.1271/bbb.60.1093
  6. Park, KY, Lee, KI and Rhee, SH. Inhibitory effect of green-yellow vegetables on the mutagenicity in Salmonella assay system and on the growth of AZ-521 human gastric cancer cells. J. Korean Soc. Food Nutr. 21:149-153. 1992
  7. Lee, KI, Rhee, SK, Park, KY and Kim, JO. Antimutagenic compound identified from perilla leaf. J. Korean Soc. Food Nutr. 21(3):302-307. 1992
  8. Park, JG, Hyun, JW, Lim, KH, Shin, JE, Won, YJ, Yi, YD, Shin, KH, Chnag, IM and Woo, WS. Antineoplastic effects from traditional medicinal plants. Korean J. Pharmacol. 24:223-230. 1993
  9. Cheung, LM, Cheung, PCK and Ooi, VEC. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem. 80:1-7. 2003 https://doi.org/10.1016/S0308-8146(02)00227-3
  10. Mau, JL, Lin, HC and Song, SF. Antioxidant properties of several speciality mushroom. Food Res. Int. 35:519-526. 2002 https://doi.org/10.1016/S0963-9969(01)00150-8
  11. Yang, JH, Lin, HC and Mau, JL. Antioxidant properties of several commercial mushroom. Food Chem. 77:229-2357. 2002 https://doi.org/10.1016/S0308-8146(01)00342-9
  12. Lie, F, Ooi, VEC and Chang, ST. Free radical scavenging activities of mushroom polysaccharide ext- racts. Life Sci. 60:763-771. 1997 https://doi.org/10.1016/S0024-3205(97)00004-0
  13. Lee, BC, Bae, JT, Pyo, HB, Choe, TB, Kim, SW, Hwang, HJ and Yun, JW. Biological activities of the polysaccharides produced from submerged culture of the edible Basidimycete Grifola frondosa. Enz. Microb. Technol. 6274:1-8. 2003
  14. Comporti, M Lipid, peroxidation: An overview. In: Free Radicals: From Basic Science to Medicine: .Molecular and cell biology updates (Poli G, Albano E & Dianzani MU, eds.), pp. 65-79. Birkhauser Verlag, Basel, Switzerland. 1993
  15. Meneghini, R, Martins, EAL and Calderaro, M. DNA damage by reactive oxygen species: The role of metal. In: Free Radicals: From Basic Science to Medicine: Molecular and cell biology updates (Poli G, Albano E & Dianzani MD, eds.), pp. 102-112. Birkhauser Verlag, Basel, Switzerland. 1993
  16. Park, YH. Effect of polyamine on modification of biomodics by aldehyde. PhD in Medicine Thesis. Seoul National University, 2000
  17. Gomez-Sanchez, A, Hermonsin, I and Mayo, I. Cleavage andoligomerization of malondialdehyde under physiological conditions. Tetrahedron Letters 28: 4077-4080. 1990
  18. Saija, A, Scalese, M, Lanza, M, Marzullo, D, Bonina F and Castelli, F. Flavonoids as antioxidant agents: Importance of their interaction with biomembranes. Free. Radical Biology & Medicine 19(4): 481-486. 1995 https://doi.org/10.1016/0891-5849(94)00240-K
  19. Haase, G and Dunkley, WL. Ascorbic acid and copper in linoleate oxidation. I. Measurement of oxidation by ultraviolet spectrophotometry and the thiobarbituric acid test. Journal of Lipid Research 10: 555-560. 1969
  20. Chen, HM, Muramoto, K, Yamauchi, F, Fujimoto, K and Nokihara, K. Antioxidative properties of histidine-containing peptides designed from peptide fragments found in the digests of a soybean protein. J. Agricultural & Food Chem. 46:49-53. 1998 https://doi.org/10.1021/jf970649w
  21. Maron, DM and Ames, BN. Revised methods for the Salmonella mutagenicity test. Mutat. Res. 113: 173-215. 1983 https://doi.org/10.1016/0165-1161(83)90010-9
  22. Mutsushima, T, Sugimura, T, Nagao, M, Yahagi, T, Shirai, A and Sawamura, M. Factors modulating mutagenicity in microbial test, In 'Short-term test, systems for detecting carcinogens'. Norphth KH and Gamer RC (eds.), Springer, Berling, p. 273. 1980
  23. Denizot, F and Lang, R. Rapid colorimetric assay for cell growth and survival modification to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 89:271-277. 1966 https://doi.org/10.1016/0022-1759(86)90368-6
  24. Song, JH, Lee, HS, Hwang, JK, Chung, TY, Hong, SR and Park, KM. Physiological activities of Phelliuns ribis Extracts. Korean J. Food Sci. Technol. 35(4):690-695. 2003
  25. Kang, YH, Park, YK and Lee, GD. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J. Food Sci. Technol. 28:232-239. 1996
  26. Bok, JW, Lee, SK, and Kim, BK. Studies of development of new pharmacologically active components of Ganoderma lucidum. J. Korean Biochem. 27:149-153. 1994
  27. Miyasaki, T and Nishijima, M. Studies on fungal polysaccharides. XVII. Structural examination of a water-soluble, antitumor polysaccharide of Ganoderma lucidum. Chem. Pharm. Bull. 29:3611. 1981
  28. Yun, TK and Kim, SH. Inhibition of development of benzo(a) pyrene-induced mouse pulmonary adenoma by several natural products in medium-term bioassay system. J. Korean Cancer Res. Assoc. 20:133. 1988
  29. Yun, TK and Lee, YS. Effects of Ganoderma lucidum on mouse pulmonary adenoma induced by benzo(a) pyrene. J. Korean Cancer Assoc. 25(4):531538. 1993
  30. Kim, JS and Lee, YS. Effects of Ganoderma lucidum extract on the development of rat liver preneoplastic lesions. Korean J. Toxicol. 5:135. 1989