Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effects of Epigallocatechin Gallate on Adhesion, Invasion and Matrix Metalloproteinase Activity in MDA-MB-231 Human Breast Cancer Cells

Epigallocatechin Gallate(EGCG)가 MDA-MB-231 인체 유방암 세포의 부착성, 침윤성과 Matrix Metalloproteinase 활성에 미치는 영향

  • Bang Myung Hee (Department of Food Science and Nutrition, Dankook University) ;
  • Kim Ji Hye (Department of Food Science and Nutrition, Dankook University) ;
  • Kim Woo Kyoung (Department of Food Science and Nutrition, Dankook University)
  • 방명희 (단국대학교 식품영양학과) ;
  • 김지혜 (단국대학교 식품영양학과) ;
  • 김우경 (단국대학교 식품영양학과)
  • Published : 2005.03.01
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Abstract

Tumor invasion is composed of four steps: cell adhesion to the extracellular matrix, degradation of the extracellular matrix components, tumor cell motility followed by cell detachment. Matrix metalloproteinases (MMPs) are important proteinases that associated with degradation of matrix component. Epigallocatechin gallate (EGCG) is a major polyphenotic constituent of green tea. In the study, we examined the anti-invasive and MMP activity suppression effects of EGCG in MDA-MB-231 human breast cancer cells. MDA-MB-23l human breast cancer cells were cultured with various concentrations 0 - 100 μM of EGCG. EGCG significantly inhibited the cell adhesion to the fibronectin. Cell motility through gelatin filter and invasion to Matrigel were inhibited dose-dependently by EGCG treatment. EGCG also inhibited the activities of MMP-2, -9 and the amount of MMP-9 (α = 0.05). Therefore, EGCG may contribute to the potential beneficial food component to prevent the invasion and metastasis in breast cancer. (Korean J Nutrition 38(2): 104～111, 2005)

Keywords

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