한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제22권1호
  • /
  • Pages.36-42
  • /
  • 2005
  • /
  • 1598-298X(pISSN)
  • /
  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

쓰레기 매립지로부터 발생된 오염이 동물 림프구의 DNA 이상에 미치는 영향

DNA Damage of Peripheral Lymphocytes of Animals Exposed with Pollution at Waste Depository

  • 김재우 (건국대학교 수의과대학) ;
  • 이병한 (건국대학교 수의과대학) ;
  • 임좌진 (건국대학교 수의과대학) ;
  • 이수한 (건국대학교 수의과대학) ;
  • 배춘식 (전남대학교 수의과대학 및 생물공학연구소) ;
  • 김진영 (건국대학교 수의과대학) ;
  • 정순옥 (건국대학교 수의과대학) ;
  • 박희명 (건국대학교 수의과대학) ;
  • 정병현 (건국대학교 수의과대학)
  • Kim Jea-woo (College of Veterinary Medicine, Konkuk University) ;
  • ;
  • Lim Joa-jin (College of Veterinary Medicine, Konkuk University) ;
  • Lee Soo-han (College of Veterinary Medicine, Konkuk University) ;
  • Bae Chun-sik (College of Veterinary Medicine, Biotechnology Research institute, Chonnam National University) ;
  • Kim Jin-young (College of Veterinary Medicine, Konkuk University) ;
  • Jeong Soon-wuk (College of Veterinary Medicine, Konkuk University) ;
  • Park Hee-myung (College of Veterinary Medicine, Konkuk University) ;
  • Chung Byung-hyun (College of Veterinary Medicine, Konkuk University)
  • 발행 : 2005.03.01
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초록

Lots of waste are produced from cities and embeded at the depository. The waste produces chemicals and organic matters. Those toxicants contaminate air and water. The pollution causes the increase of insects thereby to spray pesticide on the depository as well as its outskirts. Chemicals and pesticide ingredients are accumulated on the depository and released into outskirts. Those toxic agents are assumed to generate DNA damage to animals exposed to the water and air pollutions. To prove the possibility of DNA damage to pollution, comet assay was conducted on lymphocytes of animals exposed to the pollution of a depository at Southern part of Seoul. Peripheral lymphocytes of animals were treated with endonuclease III and electrophoresed. Broken DNA was released and measured under fluorescence microscope. The measurement showed no statistically significant DNA damage but some individuals showed higher DNA damage than in that of control group. These experiments were carried out on rabbits and dogs, the most and the least contaminated, respectively. The rate of DNA damage of cows was in between that of rabbits and dogs.

키워드

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