Journal of Animal Science and Technology

  • 제47권3호
  • /
  • Pages.363-370
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  • 2005
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  • 2672-0191(pISSN)
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  • 2055-0391(eISSN)
한국축산학회 (Korean Society of Animal Sciences and Technology)
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돼지 미성숙 난포란의 체외성숙 시 β-mercaptoethanol의 첨가가 체외성숙, 체외수정 및 Glutathione 수준에 미치는 영향

Effect of β-Mercaptoethanol Supplement during In Vitro Maturation on IVM, IVF and Glutathione Level in Porcine Oocytes

  • 오신애 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 김창근 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 정영채 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 장유민 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 방명걸 (중앙대학교 산업과학대학 동물자원과학과)
  • Oh, Shin-Ae (Department of Animal Science & Technology, College of Industrial Sciences, Chung-Ang University) ;
  • Kim, Chang-Keun (Department of Animal Science & Technology, College of Industrial Sciences, Chung-Ang University) ;
  • Chung, Yung-Chai (Department of Animal Science & Technology, College of Industrial Sciences, Chung-Ang University) ;
  • Chang, Yoo-Min (Department of Animal Science & Technology, College of Industrial Sciences, Chung-Ang University) ;
  • Pang, Myung-Geol (Department of Animal Science & Technology, College of Industrial Sciences, Chung-Ang University)
  • 발행 : 2005.06.30
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초록

Experiments were conducted to determine the effects of beta-mercaptoethanol ($\beta$-ME) supplements to the in vitro maturation (IVM) medium on in vitro fertilization (IVF) and intracellular glutathione (GSH) concentration. Porcine cumulus-intact oocytes were matured in TCM-I99 medium containing porcine follicular fluid, sodium pyruvate, D-glucose, FBS, hormonal supplements, and $\beta$-ME (0, 25, 50 and 100 ${\mu}$M) for 36 to 46h. After culture, cumulus-free matured oocytes were co-incubated with epididymal spermatozoa for 18h. There were no significant differences in the maturation rate among treatment groups. However, increases (P < 0.05) in intracellular GSH concentration before and after. fertilization were observed in 50 ${\mu}$M $\beta$-ME supplements to the IVM medium. Also, increases (P < 0.05) in male pronuclear formation after IVF were observed in same treatment group. In conclusion, supplementing $\beta$-ME into the IVM medium increased intracellular GSH concentrations and increased fertilization in vitro.

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참고문헌

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