Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Statistical Optimization of Chitinase Production by Pantoea dispersa to Enhance Degradation of Crustacean Chitin Waste

  • GOHEL (Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S. University of Baroda) ;
  • VIPUL (Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S. University of Baroda) ;
  • DERICK JIWAN (Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S. University of Baroda) ;
  • PRANAV VYAS (Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S. University of Baroda) ;
  • H. S. CHHATPAR (Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S. University of Baroda)
  • Published : 2005.02.01
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Abstract

A novel chitinase-producing bacterial strain of Pantoea dispersa was isolated from the sea near Bhavnagar, India for efficient disposal of chitinous waste from the seafood processing industry. The medium components were optimized by using a cubic model in the central composite design for increasing chitinase production. The optimal concentrations for higher production of chitinase were (g l-1) chitin, 10.0; urea, 0.35; MgSO4 7H2O, 0.08, and CaCl2, 0.15. Here, peptone (0.05 g l-1) was used as a constant variant in all trials. Using a statistical optimization method, the chitinase production was found to increase from 108 to 486.4 units ml-1. Chitin was prepared from the crustacean waste, and Fourier Transform Infrared (FTIR) Spectroscopy was used to characterize the isolated chitin. Chitinous waste degradation was studied in terms of chitinase production.

Keywords

References

  1. Bhushan, B. 2000. Production and characterization of thermostable chitinase from a new alkalophilic Bacillus sp. BG-11. J. Appl. Microbiol. 88: 800- 808 https://doi.org/10.1046/j.1365-2672.2000.01016.x
  2. Dani, V. R. 1992. Important chemical group for chitin detection by IR, 4th Ed., Organic Spectroscopy. Tata McGraw-Hill publishing company, New Delhi
  3. Holt, J. G., N. R. Krieg, P. H. Sneath, J. T. Staley, and S. T. Williams. 1994. Bergey's Manual of Determinative Bacteriology, 9th Ed. Williams and Wilkins, Maryland
  4. James, M. 1976. Which response surface design is best. Technomet. 18: 411- 417 https://doi.org/10.2307/1268656
  5. Kole, M. M. and I. Altosaar. 1985. Increased chitinase production by a non-pigmented mutant of Serratia marcescens. FEMS Microbiol. Lett. 26: 265- 269 https://doi.org/10.1111/j.1574-6968.1985.tb01608.x
  6. Marcin, H. S., R. Hahlweg, and G. P. Martin. 2000. Comparative analysis of chitosans from insects and crustacea. Adv. Chitin Sci. 4: 40- 49
  7. Monreal, J. and E. T. Reese. 1969. The chitinase of Serratia marcescens. Can. J. Microbiol. 15: 689- 696 https://doi.org/10.1139/m69-122
  8. Muzzarelli, R. A. A. 1997. Chitin. Pergamon Press, Oxford
  9. No, H. K. and S. P. Meyers. 1997. Preparation of chitin and chitosan, pp. 475- 489. In R. A. A. Muzzarelli and M. G. Peter (eds.). Chitin Hand book. Atec, Grottammare, Italy
  10. Patil, S. R., V. Ghormade, and M. V. Deshpande. 2000. Chitinolytic enzyme: An exploration. Enzyme Microb. Technol. 26: 473- 483 https://doi.org/10.1016/S0141-0229(00)00134-4
  11. Plackett, R. L. and J. P. Burman. 1946. The design of optimum multifactorial 24 experiments. Biomet. 37: 305-25
  12. Pujari, V. and T. S. Chandra. 2000. Statistical optimization of medium components for enhanced riboflavin production by a UV-mutant of Eremothecium ashbyii. Process. Biochem. 36:31-37 https://doi.org/10.1016/S0032-9592(00)00173-4
  13. Rao, K. J., C. H. Kim, and S. K. Rhee. 2000. Statistical optimization of medium for the production of recombinant hirudin from Saccharomyces cerevisiae using response surface methodology. Process. Biochem. 35: 639- 647 https://doi.org/10.1016/S0032-9592(99)00129-6
  14. Sabry. 1992. Microbial degradation of shrimp-shell waste. J. Basic Microbiol. 32: 107- 115 https://doi.org/10.1002/jobm.3620320207
  15. Shaikh, S. A. and M. V. Deshpande. 1993. Chitinolytic enzyme: An exploration. Enzyme Microb. Technol. 9: 468-475
  16. Suresh, P. V. and M. Chandrasekaran. 1998. Utilization of prawn waste for chitinase production by the marine fungus Beauveria bassiana by solid state fermentation. World J. Microbiol. Biotechnol. 14: 655- 660 https://doi.org/10.1023/A:1008844516915
  17. Vaidya, R. J., S. L. A. Macmil, P. R. Vyas, and H. S. Chhatpar. 2003. The novel method for isolating chitinolytic bacteria and its application in screening for hyperchitinase producing mutant of Alcaligenes xylosoxydans. Lett. Appl. Microbiol. 36: 129- 134 https://doi.org/10.1046/j.1472-765X.2003.01274.x
  18. Vyas, P. and M. V. Deshpande. 1989. Chitinase production by Myrothecium verruca ria and its significance for fungal mycelia degradation. J. Gen. Appl. Microbiol. 35: 343- 350 https://doi.org/10.2323/jgam.35.343