Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Hypoglycemic Effect of Collybia confluens Exobiopolymer Produced by Submerged Mycelial Culture on Diabetic Rats

  • Published : 2005.02.01
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Abstract

The hypoglycemic effect of Collybia confluens exobiopolymer was investigated in streptozotocin (STZ)-induced diabetic rats. In a dose-dependent study, the exobiopolymer, at 150 mg/kg body weight (BW) dose substantially lowered the plasma glucose level by 29.3%, as compared to the control group. It also lowered the plasma total cholesterol and triglyceride levels by 23.3 and 30.7%, respectively, and reduced the liver total cholesterol and triglyceride levels by 23.0 and 33.5%, respectively. The activity of alanine transaminase (ALT) and aspartate transaminase (AST) was reduced 34.6% and 23.6% respectively, by the exobiopolymer administration, compared to the control group. The exobiopolymer was found to contain 83.2% carbohydrate and 16.8% protein. The sugar and amino acid of the exobiopolymer were also analyzed in detail.

Keywords

References

  1. Bar-On, H., P. S. Roheim, and H. A. Eder. 1976. Hyperlipoproteinemia in streptozotocin-treated rats. Diabetes 25: 509- 515 https://doi.org/10.2337/diabetes.25.6.509
  2. Bursch, W. and R. Schulte-Hermann. 1986. Cytoprotective effect of the prostacyclin derivative Hoprost against liver cell death induced by the hepatotoxins carbon tetrachloride and bromobenzene. Klin. Wochenschr. 64: 47- 50
  3. Chihara, G., Y. Maeda, J. Hamuro, T. Sasaki, and F. Fukuoka. 1969. Inhibition of mouse sarcoma 180 by polysaccharides from Lentinus edodes (Berk.) sing. Nature 222: 687- 688 https://doi.org/10.1038/222687a0
  4. Dubois, M., K. Gilles, J. K. Hamilton, P. A. Rebers, and F. Smith. 1951. A colorimetric method for determination of sugar. Nature 168: 167 https://doi.org/10.1038/168167a0
  5. Duncan, D. M. 1957. Multiple-range tests for correlated and heteroscedastic means. Biometrics 13: 164- 170 https://doi.org/10.2307/2527799
  6. Folch, J., M. Lees, and G. H. Sloane-Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226: 497- 509
  7. Furuse, M., C. Kimura, R. T. Mabayo, H. Takahashi, and J. Okumura. 1993. Dietary sorbose prevents and improves hyperglycemia in genetically diabetic mice. J. Nutr: 123: 59- 65 https://doi.org/10.1093/jn/123.1.59
  8. Hikino, H., M. Ishiyama, Y. Suzuki, and C. Konno. 1989. Mechanisms of hypoglycemic activity of Ganoderan B: A glycan of Ganoderma lucidum fruit bodies. Planta Med. 55: 423-428 https://doi.org/10.1055/s-2006-962057
  9. Johnson, I. M. and J. M. Gee. 1981. Effect of gel-forming gums on the intestinal un stirred layer and sugar transport in vitro. Gut 22: 398- 403 https://doi.org/10.1136/gut.22.5.398
  10. Jones, T. M. and P. O. Albersheim. 1972. A gas chromatographic method for the determination of aldose and uronic acid constituents of plant cell wall polysaccharides. Plant Physiol. 49: 926- 936 https://doi.org/10.1104/pp.49.6.926
  11. Junod, A., A. E. Lambert, L. Orci, R. Pietet, A E. Gonet, and A E. Renold. 1967. Studies of the diabetogenic action of streptozotocin. proc. Soc. Exp. Biol Med. 126: 201- 205
  12. Kabir, Y., M. Yamaguchi, and S. Kimura. 1987. Effect of Shiitake (Lentinus edodes) and Maitake (Grifola frondosa) mushrooms on blood pressure and plasma lipids of spontaneously hypertensive rats. J. Nutr. Sci. Vitaminol 33: 341- 346 https://doi.org/10.3177/jnsv.33.341
  13. Kiho, T., H. Morimoto, M. Sakushima, S. Usui, and S. Ukai. 1995. Polysaccharides in fungi. XXXV. Anti-diabetic activity of and acidic polysaccharide from the fruiting bodies of Tremella aurantia. Biol. Pharm. Bull. 18: 1627- 1629 https://doi.org/10.1248/bpb.18.1627
  14. Kiho, T., K. Ookubo, S. Usui, S. Ukai, and K. Hirano. 1999. Structural features and hypoglycemic activity of a polysaccharide (CS-F10) from the cultured mycelium of Cordyceps sinensis. Biol .Pharm. Bull. 22: 966- 970 https://doi.org/10.1248/bpb.22.966
  15. Kiho, T., S. Itahashi, M. Sakushima, T. Matsunaga, S. Usui, H. Mori, H. Sakamoto, and Y. Ishiguro. 1997. Polysaccharide in fungi. XXXVIII. Anti-diabetic activity and structural feature of galactomannan elaborated by Pestalotiopsis species. Biol. Pharm. Bull. 20: 118-121 https://doi.org/10.1248/bpb.20.118
  16. Kiho, T., S. Sobue, and S. Ukai. 1994. Structural features and hypoglycemic activities of two polysaccharides from a hot-water extract of Agrocybe cylindracea. Carbohydr. Res. 251:81-87 https://doi.org/10.1016/0008-6215(94)84277-9
  17. Kim, D. H., B. K. Yang, S. C. Jeong, N. J. Hur, S. Das, J. W. Yun, J. W. Choi, Y. S. Lee, and C. H. Song. 2001. A preliminary study on the hypoglycemic effect of the exopolymers produced by five different medicinal mushrooms. J. Microbiol Biotechnol 11: 167- 171
  18. Kim, S. H., J. S. Kim, M. R. Jin, H. W. Kim, E. C. Choi, and B. K. Kim. 1993. Studies on antitumor components of Collyhia confluens. Kor. J. Pharmacogn. 24: 267 - 281
  19. Kim, S. H., H. W. Kim, E. C. Choi, and B. K. Kim. 1993. Immunologcial studies on Collyban of Collyhia confluens. J. Kor. Cancer Association 25: 288- 298
  20. Kimura, Y., H. Okuda, and S. Arichi, 1988. Effects of the extracts of Ganoderma lucidum on blood glucose level in rats. Planta Med. 54: 290- 294 https://doi.org/10.1055/s-2006-962435
  21. Lorenzen, K. and T. Anke. 1998. Basidiomycetes as a source for new bioactive natural products. Curr. Org. Chem. 2: 329- 364
  22. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265- 275
  23. Nelson, R. W. 1989. The role of fiber in managing diabetes mellitus. Vet. Med. 84: 1156- 1160
  24. Omodeo-Sale, F., S. Marchesini, P. H. Fishman, and B. Berra. 1984. A sensitive enzymatic assay for determination of cholesterol in lipid extracts. Anal. Biochem. 142: 347-350 https://doi.org/10.1016/0003-2697(84)90475-5
  25. Park, J. K., H. J. Cho, Y. G. Lim, Y. H. Cho, and C. H. Lee. 2002. Hypocholesterolemic effect of CJ90002 in hamsters: A potent inhibitor for squalene synthase from Paeonia moutan. J. Microbiol. Biotechnol. 12: 222- 227
  26. Raabo, E. and T. C. Terkildsen. 1960. On the enzymatic determination of blood glucose. Scand. J. Clin. Lab. Invest. 12: 402- 407 https://doi.org/10.3109/00365516009065404
  27. Simon, B., T. Anke, U. Anders, M. Neuhaus, and F. Hansske. 1995. Collybial, a new antibiotic sesquiterpenoid from Collybia confluens (Basidiomycetes). Z. Naturforsch. (C) 50: 173- 180
  28. Takasu, N., I. Korniya, T. Asawa, Y. Nagasawa, and T. Yamada. 1991. Streptozotocin and alloxan-induced $H_{2}O_{2}$ generation and DNA fragmentation in pancreatic islets. Diabetes 40: 1141- 1145 https://doi.org/10.2337/diabetes.40.9.1141
  29. Van Horn, L. V. 1996. Lipid metabolism and choices for persons with diabetes, pp. 336- 359. In Powers, M. A. (ed.), Handbook of Diabetes Medical Nutrition Therapy. Aspen Publishers Inc., Gaithersburg, Maryland
  30. Waters Associates. 1980. Official method of amino acid analysis. Amino acid analysis system in the operation manual of Waters Associates, p. 37. U.S.A.
  31. Yang, B. K., D. H. Kim, S. C. Jeong, S. Das, Y. S. Choi, J. S. Shin, S. C. Lee, and C. H. Song. 2002. Hypoglycemic effect of a Lentinus edodes exo-polyrner produced from submerged mycelial culture. Biosci. Biotechnol. Biochem. 66: 937- 942 https://doi.org/10.1271/bbb.66.937
  32. Yang, B. K., J. B. Park, and C. H. Song. 2002. Hypolipidemic effect of exo-polymer produced in submerged mycelial culture of five different mushroom. J. Microbiol. Biotechnol. 12: 957-961
  33. Yang, B. K., S. C. Jeong, and C. H. Song. 2002. Hypolipidemic effect of exo- and endo-biopolymers produced from submerged mycelial culture of Ganoderma lucidum in rats. J. Microbiol. Biotechnol. 12: 872- 877
  34. Zhang, X. H., H. B. Hu, Y. L. Tang, R. S. Huang, J. F. Luo, and B. K. Hur. 2002. The hepatoprotective effects of polysaccharides isolated from submerged fermentation of Ganoderma lucidum. J. Microbiol. Biotechnol. 12: 367-370
  35. Yang, B.-K., M. A. Wilson, K. Y. Cho, and C.-H. Song. 2004. Hypoglycemic effect of exo- and endo-biopolymers produced by submerged mycelial culture of Ganoderma lucidum in streptozotocin-induced diabetic rats. J. Microbiol. Biotechnol. 14: 972- 977