Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Gibberellins-Producing Fungi from the Root of Several Sesamum indicum Plants

  • CHOI, WHA-YOUL (Department of Microbiology, Kyungpook National University) ;
  • RIM, SOON-OK (Department of Microbiology, Kyungpook National University) ;
  • LEE, JIN-HYUNG (Department of Microbiology, Kyungpook National University) ;
  • LEE, JIN-MAN (Department of Microbiology, Kyungpook National University) ;
  • LEE, IN-JUNG (Department of Agriculture, Kyungpook National University) ;
  • CHO, KANG-JIN (National Institute of Agricultural Biotechnology) ;
  • RHEE, IN-KOO (Department of Agricultural Chemistry, Kyungpook National University) ;
  • KWON, JUNG-BAE (Institute for Bioresources Research Gyeongbuk Provincial A.T.A.) ;
  • KIM, JONG-GUK (Department of Microbiology, Kyungpook National University)
  • Published : 2005.02.01
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Abstract

Gibberellins (GAs) play important roles in plant growth and development. Fifty-four fungi were isolated from the roots of 4 kinds of Sesamum indicum plants, and the production of GAs was spectrophotometrically examined. The number of GA-producing fungi was two strains from S. indicum, four strains from Gold S. indicum, and five strains from Brown S. indicum. Eleven fungi with GAs-producing activity were incubated for seven days in 40 ml of Czapek's liquid medium at $25^{\circ}C$ and 120 rpm, and the amount of each GA in the medium was measured by gas chromatographymass spectrometery (GC-MS). Penicillium commune KNU5379 produced more $GA_3$, $GA_4$, and $GA_7$ than Gibberella fujikuroi, Fusarium proliferatum, and Neurospora crassa which are known as GAs-producing fungi. GAs-producing activity of the P. commune KNU5379 was shown to produce 71.69 ng of $GA_1$, 252.42 ng of $GA_3$, 612.00 ng of $GA_4$, 259.00 ng of $GA_7$, and 202.69 ng of $GA_9$ in 25 ml of liquid medium. Bioassay of culture fluid of GA-producing fungi was also performed on rice sprout.

Keywords

References

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