Journal of Photoscience
- Volume 12 Issue 1
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- Pages.1-9
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- 2005
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- 1225-8555(pISSN)
Cloning and Expression Characteristics of Pharbitis nil COP1 (PnCOP1) During the Floral Induction
- Kim, Yun-Hee (Department of Biology, School of Biosciences and Biotechnology, Chungnam National University) ;
- Kim, Seong-Ryong (Department of Biology, School of Biosciences and Biotechnology, Chungnam National University) ;
- Heo, Yoon-Kang (Department of Biology, School of Biosciences and Biotechnology, Chungnam National University)
- Published : 2005.04.01
Abstract
The ubiquitin E3 ligase COP1 (Constitutive Photomorphogenesis 1) is a protein repressor of photomorphogenesis in Arabidopsisplants, and it found in various organisms, including animals. The COP1 protein regulates the stability of many of the light-signaling components that are involved in photomorphogenesis and in the developmental processes. To study the effect of COP1 on flowering in a short day plant, we have cloned a full-length of PnCOP1 (Pharbitis nil COP1) cDNA from Pharbitis nil Choisy cv. Violet, and we examined its transcript levels under various conditions. A full-length PnCOP1 cDNA consists of 2,280 bp nucleotidesthat contain 47 bp of 5'-UTR, 232 bp of 3'-UTR including the poly (A) tail, and 1,998 bp of the coding sequence. The deduced amino acid sequence contains 666 amino acids, giving it a theoretical molecular weight of 75 kD and a isolectric point of 6.2. The PnCOP1 contains three distinct domains, an N-terminal