The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Biological Characterization and Sequence Analysis of Cucumber mosaic virus isolated from Capsicum annuum

  • Kim, Min-Jea (Plant Virus GenBank, Division of Environmental & Life Sciences, Seoul Women's University) ;
  • Choi, Seung-Kook (USDA-ARS, Department of Plant Pathology, Cornell University) ;
  • Yoon, Ju-Yeon (Plant Virus GenBank, Division of Environmental & Life Sciences, Seoul Women's University) ;
  • Choi, Jang-Kyung (Division of Biological Environment, Kangwon National University) ;
  • Ryu, Ki-Hyun (Plant Virus GenBank, Division of Environmental & Life Sciences, Seoul Women's University)
  • Published : 2005.01.01
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Abstract

Whereas most of isolates of Cucumber mosaic virus(CMV) can induce green mosaic systemic symptoms on zucchini squash, foliar symptoms of a pepper isolate of CMV (Pf-CMV)-infected zucchini squash revealed systemic chlorotic spots. To assess this biological property, infectious full-length cDNA clones of Pf-CMV were constructed using long-template RT-PCR. The complete nucleotide sequences of RNA2 and RNA3 of Pf-CMV were determined from the infectious fulllength cDNA clones, respectively. RNA 2 and RNA3 of Pf-CMV contain 3,070 nucleotides and 2,213 nucleotides, respectively. Overall sequence homology of two RNAs revealed high similarity (90%) between CMV strains, and 60% similarity to those of Tomato aspermy virus and Peanut stunt virus strains. By sequence analysis with known representative strains of CMV, Pf- CMV belongs to a typical member of CMV subgroup IA. The virus has high evolutionary relationship with Fny-CMV, but the pathology of Pf-CMV in zucchini squash was quite different from that of Fny-CMV. The pesudorecombinant virus, F1P2P3, induced chlorotic spot leaf symptom and timing of systemic symptom in squash plants, similar to the plants infected by Pf-CMV. No systemic symptoms were observed when Pf-CMVinoculated cotyledons were removed at 5 days postinoculation (dpi) while Fny-CMV showed systemic symptom at 2 dpi. These results suggest that the pepper isolate of CMV possesses unique pathological properties distinguishable to other isolates of CMVs in zucchini squash.

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References

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