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The Beneficial Effects of Pectin on Obesity In vitro and In vivo

In vitro 및 In vivo에서 펙틴의 비만 억제 효과

  • 권진영 (부산대학교 식품영양학과 및 김치연구소) ;
  • 안인숙 (한동대학교 생명공학연구소) ;
  • 박건영 (부산대학교 식품영양학과 및 김치연구소) ;
  • 최홍식 (부산대학교 식품영양학과 및 김치연구소) ;
  • 송영옥 (부산대학교 식품영양학과 및 김치연구소)
  • Published : 2005.01.01

Abstract

The effects of pectin on obesity was studied using 3T3-L1 pre-adipocytes and rats fed 20% high fat diets. The concentration of leptin released from 3T3-L1 adipocytes in the presence of pectin was significantly decreased by 85% compared to that of the control (p<0.05), however, glycerol concentration was not changed. These data indicate that pectin seems to inhibit lipids accumulation in the adipocytes rather than enhance the lipolytic activity. Forty Sprague Dawley rats were fed 20% high fat diet for 8 weeks to induce obesity and then divided equally into four groups. Experimental groups were normal diet group (ND), high fat diet group (HFD), HDF with 10% pectin group (HFP10), and HDF with 20% pectin group (HFP20). Diet for the each group was prepared to be iso-caloric following AIN-76 guideline. After obesity was induced, rats were placed on an restricted diet for 9 weeks. The body weight of HFD increased 50% (p<0.05) compared to the ND, while it was decreased by 12% and 16% for HFP10 and HFP20, respectively (p<0.05). The relative amount of visceral fats for HFDl0 and HFD20 were decreased by 45% and 59% compared to that of HDF (130%), respectively (p<0.05). Pectin seems to have a greater effect on reducing visceral fats accumulation than weight reduction. Significantly increased level of triglyceride, total cholesterol or LDL-cholesterol in the plasma of HFD was returned to the normal or even below the normal by pectin diet, while the level of HDL-cholesterol increased. Lipid lowering effect was also observed in the liver and heart. These effects of pectin were dosedependent. In conclusion, the beneficial effect of pectin on the obesity was observed from cell culture experiment and animal study in terms of inhibiting the accumulation of lipids in the adipocytes.

펙틴의 비만억제 효과 및 지질저하 효과를 3T3-Ll adipocyte cell culture system과 20% 고지방식이를 섭취시킨 흰쥐에서 살펴보았다 펙틴을 첨가한 3T3-Ll adipocyte cell의 글리세롤 농도는 대조군에 비해 유의적인 차이가 없었으나, leptin의 농도는 83% 유의 적으로 감소하여 (p<0.01) adipose 세포에 지방 축적을 억제하는 효과가 있음이 확인되었다. 흰쥐를 이용한 동물실험에서 실험군간의 식이섭취량에는 차이가 없었으나 20% 지방을 섭취시킨 쥐는 정상식이군에 비해 체중이 50% 증가하였고, 고지방식이에 펙틴을 10%와 20% 첨가시킨 군에서 는 체중이 각각 12% 및 16% 감소하였다. 상대적인 내장지방의 함량(g/100 g body weight)은 ND 4.3 g, ITFD 5.6 g, HFP10은 3.1 g, HFP20은 2.3 g로 펙틴 첨가군의 상대적인 내장 지방 함량이 정상대조군보다 낮아 펙틴의 비만억제 효과가 현저하였다 고지방식이에 의해 상승된 혈장 중성 지질, 총 콜레스테롤 및 LDL-콜레스테롤 농도는 펙틴 첨가에 의해 감소되었고, HDL-콜레스테롤은 증가하여 펙틴의 첨가에 의한 지질 개선 효과가 관찰되었다. 간 및 심장의 지질 농도 역시 고지방식이에 의해 증가하였으나 펙틴의 첨가에 의해 감소되었다. 특히 분변으로의 지질 배설 현상은 펙틴 첨가군에서 현저하게 나타났는데 이러한 펙틴의 효과는 첨가 농도 의존적으로 관찰되었다. 펙틴의 비만 억제 효과내장지방의 축적을 억제하고, 소장에서 지질의 흡수를 방해하여 분변으로의 지질 배설을 촉진시키는 이외에도 다른 생리 활성이 있을 것으로 생각된다. 이상의 결과를 살펴보면 펙틴은 고지방식이를 섭취 하는 경우 복부지방 축적을 억제하는 효과가 체중감소 효과보다 크며 혈장 중성지질, 콜레스테롤 LDL 콜레스테롤을 떨어뜨리고 HDL 콜레스테롤은 상승시키는 효과가 현저한 것으로 나타났다.구 결과, cook-chill생산 시 녹차 추출물의 첨가가 미생물적 품질유지에 효과가 있다고 사료되는 바 본 연구결과를 기초로 급식소에서 음식 생산 시 녹차 추출물 및 천연 항균성 물질 첨가에 따른 미생물적 품질 및 관능적 품질검사를 통한 레시피 개발에 관한 지속적인 연구가 수행되어야 하겠다.다.다리다보니 점심시간을 활용할 수 없게 되는 문제점에 대한 재검토가 필요하다. 따라서 차후 학교급식의 안전성 확보를 위한 급식환경 개선의 일환으로 식당공간 확보 시 신속한 시간 내에 급식이 가능하도록 넓은 공간과 쾌적한 환경의 식당 조성에 대해 관심을 기울여야 할 것으로 사료된다. 이상 여부를 반영하는 임상증상의 빈도가 높은 청소년기 남녀 중학생의 경우 아침과 저녁의 결식빈도 및 외식과 간식의 빈도가 높았고, 아침식사의 질과 체형만족도가 낮은 것으로 나타나 청소년의 건강과 식습관 및 체형만족도가 상호 관련성이 높은 것으로 나타났다. 따라서 본 연구 결과는 성장기 청소년의 건강 유지를 위하여 바람직한 식습관의 중요성을 재인식할 수 있었으며, 올바른 식습관 확립을 위한 영양교육의 중요성이 재확인되었다.경제적일 것으로 판단된다.er 90 % of good relative dynamic modulus of elasticity due to fineness of formation caused by the increase of the unit powder content and the improvement of flowability, without regard to the replacement of crushed stone fines. Therefore, it can be said that the usage of crushed stone fines can control the strength of super flowing concrete by replacement and reduce heat of hydration.

Keywords

References

  1. Aro A, Uusitupa M, Voutilainen E, Korhonen T. 1984. Effects of guar gum in male subjects with hypercholesterolemia. Am J Clin Nutr 39: 911-916
  2. Kay M, Truswell AS. 1977. Effect of citrus pectin on blood lipids and fecal steroid excretion in man. Am J Clin Nutr 30: 171-175
  3. Judd PA, Truswell AS. 1985. The hypocholesterolemic effect of pectins in rats. Br J Nutr 53: 409-424 https://doi.org/10.1079/BJN19850051
  4. Fernandez ML, Lin ECK, Trejo A, McNamara DJ. 1992. Prickly pear (Opuntia sp) pectin reverses low density lipoprotein receptor suppression induced by a hypercholesterolemic diet in guinea pigs. J Nutr 122: 2330-2340
  5. Fernandez ML, Sun DM, Tosca MA, McNamara DJ. 1994. Citrus pectin and cholesterol interact to regualte hepatic cholesterol homeostasis and lipoprotein metabolism: A dose-response study in guinea pigs. Am J Clin Nutr 59: 869-878
  6. Cassidy MM, Calvert RJ. 1993. Effects of dietary fiber on intestinal absorption of lipids. In CRC handbook of dietary fiber in human nutrition. 2nd ed. Spiller GA, ed. CRC press, New York. p 153-162
  7. Imaizumi K, Tominaga A, Maivatari K, Sugano M. 1982. Effect of cellulose and guar gum on the secretion of mesenteric lymph chylomicrons in meal-fed rats. Nutr Rep Int 26: 263-269
  8. Cassidy MM, Lightfoot FG, Vahouny GV. 1982. Morphological aspects of dietary fiber in the intestine. Adv Lipid Res 19: 203-229 https://doi.org/10.1016/B978-0-12-024919-0.50013-8
  9. Schwartz SE, Starr C, Bachman S, Holtzapple PG. 1983 Dietary fiber decreases cholesterol and phospholipid synthesis in rats intestine. J Lipid Res 24: 746-752
  10. Garcia-Diez F, Garcia-Mediavilla G, Bayon JE, Gonzalez-Gallego G. 1996. Pectin feeding influences fecal bile acid excretion, hepatic bile acid and cholesterol synthesis and serum cholesterol in rats. J Nutr 126: 1766-1771
  11. Abbey M, Triantafilidis C, Topping DL. 1993. Dietary non-starch polysaccharides interact with cholesterol and fish oil in their effects on plasma lipids and hepatic lipoprotein receptor activity in rats. J Nutr 123: 900-908
  12. Wilson JN, Wilson SP, Eaton RP. 1984. Dietary fiber and lipoprotein metabolism in the genetically obese Zucker rat. Arteriosclerosis 4: 147-153 https://doi.org/10.1161/01.ATV.4.2.147
  13. Rotenberg S, Jakobsen PE. 1978. The effect of dietary pectin on lipid composition of blood, skeletal muscle and internal organs of rats. J Nutr 108: 1384-1392
  14. Bueno AAR, Cappel TG, Sumvold GD, Moxley RA, Reinhart GA, Clemens ET. 2000. Feline colonic microbes and fatty acid transport: Effects of feeding cellulose, beet pulp and pectin/gum arabic fibers. Nutr Res 20: 1319-1328 https://doi.org/10.1016/S0271-5317(00)00211-6
  15. Cater JW, Hardman WE, Heiuman DW, Cameron IL. 1998. Type and amount of individual dietary fibers on: serum lipid profiles, serum glucose concentration and energy intake in rats. Nutr Res 18: 1743-1756 https://doi.org/10.1016/S0271-5317(98)00142-0
  16. Bonfield CT. 1995. Dietary fiber and body management. In Dietary fiber in health and disease. Kritchevsky D, Bonfield CT, eds. Eagan Press, Washington, DC. p 459-465
  17. Knopp RH, Superko HR, Davidson M, Insull W, Dujpvne CA, Kwiterovich PO, Zavoral JH, Graham K, O'Connor RR, Edelman DA. 1999. Long-term blood cholesterol-lowering effects of a dietary fiber supplement. Am J Prev Med 17: 18-23 https://doi.org/10.1016/S0749-3797(99)00039-2
  18. Yamada H. 1994. Pectic polysaccharides from Chinese herbs: structure and biological activity. Cabohydrate Polymers 25: 269-276 https://doi.org/10.1016/0144-8617(94)90052-3
  19. Pienata KJ, Naik H, Akhtar A, Yamazaki K, Replogle TS, Lehr J, Donat LT, Tait L, Hogan V, Raz A. 1995. Inhibition of spontaneous metastasis in a rat prostate cancer model by oral administration of modified citrus pectin. J Natl Cancer Inst 87: 348-35 https://doi.org/10.1093/jnci/87.5.348
  20. Hirano M, Kiyohara H, Matsumoto T, Yamada H. 1994. Structural studies of endopolygalacturonase-resistant fragments of an antiulcer pectin from the roots of Bupleurum falcatum L. Carbohydrate Res 251: 145-162 https://doi.org/10.1016/0008-6215(94)84282-5
  21. Caro JF, Sinha MK, Kolaczynski JW, Zhang P, Considine RV. 1996. Leptin: The tale of an obesity gene. Diabetes 45: 1455-1462 https://doi.org/10.2337/diab.45.11.1455
  22. Considine RV, Sinha MK, Heiman ML, Kriau-ciunas A, Stephens TW, Nyce MR, Ohannesian JP, Marco CC, McKee LJ, Caro JF. 1996. Serum immunoreactive-leptin concentrations in normal-weight and obese humans. N Engl J Med 334: 292-295 https://doi.org/10.1056/NEJM199602013340503
  23. Folch IL, Stanley GH. 1956. A simple method for the isolation and purification of total lipids from animal tissue. J Biochem 223: 497-509
  24. Buege JA, Aust SD. 1978. Microsomal lipid peroxidation. In Methods in Enzymol. Academic Press, New York. Vol 52, p 302-310
  25. Carroll KK. 1986. Biological effects of fish oils in relation to chronic disease. Lipids 21: 731-732 https://doi.org/10.1007/BF02535402
  26. Aebi H. 1984. Catalase in vitro. In Methods in Enzymology. Academic Press, Orlando, Florida. Vol 105, p 121-126
  27. Oyanagui Y. 1984. Reevalutation of assay methods and establishment of kit for superoxide dismutase activity. Anal Biochem 142: 290-296 https://doi.org/10.1016/0003-2697(84)90467-6

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