한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제34권2호
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  • Pages.261-266
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  • 2005
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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고선량 조사된 시판 분말죽의 유전독성학적 안전성평가

Genotoxicological Safety of High-Dose Irradiated Porridges

  • 발행 : 2005.02.01
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초록

분말죽의 위생화 및 물리적 특성 개선을 위해 감마선조사 기술의 이용가능성이 높아짐에 따라 이들의 안전성을 확보할 목적으로 30 kGy 고선량 감마선조사 분말죽의 유전독성학적 안전성 평가를 실시하였다. 감마선 조사 및 비조사 분말죽의 S. Typhimurium TA98, TA100, TA1535 및 TA1537에 대한 복귀변이 집락수를 조사한 결과, 대사활성계 도입 및 부재시 모두 시험적용 농도인 0.625∼10mg/plate의 범위에서 복귀변이 집락수의 농도 의존적인 증가 혹은 감소를 보이지 않아 감마선 조사 분말죽(30 kGy)은 돌연변이원성이 없는 것으로 판단되었다. 또한, 설치류 망상적혈구를 이용하여 감마선 조사된 분말죽의 소핵 형 성시험을 수행한 결과, 감마선 조사 분말죽은 시험적용 용량인 625∼5,000 mg/kg의 범위에서 소핵을 가진 망상적혈구의 출현율이 음성대조군과 유의한 차이를 나타내지 않아 소핵을유발하지 않음을 확인하였다. 포유류 배양세포를 이용한 염색체 이상시험에서도 감마선 조사 분말죽(30 kGy)은 시험적용 용량에서 염색체이상 유발능이 5% 미만이었다.

Gamma irradiation at 30 kGy was applied to porridge to evaluate its possible genotoxicity. The genotoxicity of irradiated porridge was evaluated by Salmonella Typhimurium reversion assay, chromosomal aberration test and in vivo micronucleus assay. The results were negative in the bacterial reversion assay with S. Typhimurium TA98, TA100, TA1535 and TA1537. No mutagenicity was detected in the assay both with and without metabolic activation. In chromosomal aberration tests with CHL cells and in vivo mouse micronucleus assay, no significant difference in the incidences of chromosomal aberration and micronuclei was observed between nonirradiated and 30 kGy-irradiated porridge. These results indicate that porridge irradiated at 30 kGy did not show any genotoxic effects under these experimental conditions.

키워드

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피인용 문헌

  1. Scientific Opinion on the Chemical Safety of Irradiation of Food vol.9, pp.4, 2011, https://doi.org/10.2903/j.efsa.2011.1930
  2. Toxicity of 30 kGy Irradiated Cereal Powders for Three Months vol.37, pp.10, 2008, https://doi.org/10.3746/jkfn.2008.37.10.1264