Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Genotoxicological Safety of High-Dose Irradiated Porridges

고선량 조사된 시판 분말죽의 유전독성학적 안전성평가

  • Published : 2005.02.01
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Abstract

Gamma irradiation at 30 kGy was applied to porridge to evaluate its possible genotoxicity. The genotoxicity of irradiated porridge was evaluated by Salmonella Typhimurium reversion assay, chromosomal aberration test and in vivo micronucleus assay. The results were negative in the bacterial reversion assay with S. Typhimurium TA98, TA100, TA1535 and TA1537. No mutagenicity was detected in the assay both with and without metabolic activation. In chromosomal aberration tests with CHL cells and in vivo mouse micronucleus assay, no significant difference in the incidences of chromosomal aberration and micronuclei was observed between nonirradiated and 30 kGy-irradiated porridge. These results indicate that porridge irradiated at 30 kGy did not show any genotoxic effects under these experimental conditions.

분말죽의 위생화 및 물리적 특성 개선을 위해 감마선조사 기술의 이용가능성이 높아짐에 따라 이들의 안전성을 확보할 목적으로 30 kGy 고선량 감마선조사 분말죽의 유전독성학적 안전성 평가를 실시하였다. 감마선 조사 및 비조사 분말죽의 S. Typhimurium TA98, TA100, TA1535 및 TA1537에 대한 복귀변이 집락수를 조사한 결과, 대사활성계 도입 및 부재시 모두 시험적용 농도인 0.625∼10mg/plate의 범위에서 복귀변이 집락수의 농도 의존적인 증가 혹은 감소를 보이지 않아 감마선 조사 분말죽(30 kGy)은 돌연변이원성이 없는 것으로 판단되었다. 또한, 설치류 망상적혈구를 이용하여 감마선 조사된 분말죽의 소핵 형 성시험을 수행한 결과, 감마선 조사 분말죽은 시험적용 용량인 625∼5,000 mg/kg의 범위에서 소핵을 가진 망상적혈구의 출현율이 음성대조군과 유의한 차이를 나타내지 않아 소핵을유발하지 않음을 확인하였다. 포유류 배양세포를 이용한 염색체 이상시험에서도 감마선 조사 분말죽(30 kGy)은 시험적용 용량에서 염색체이상 유발능이 5% 미만이었다.

Keywords

References

  1. WHO. 1981. Wholesomeness of irradiated food. Report of a Joint FAO/IAEA/WHO expert committee on the wholesomeness of irradiated food. Technical Report Series, WHO, Geneva. p 659-682
  2. Bruhn C. 1995. Consumer attitudes and market response to irradiated food. J Food Protect 58: 175-179 https://doi.org/10.4315/0362-028X-58.2.175
  3. Hugo WB. 1991. A brief history of heat and chemical preservation and disinfection. J Applied Bacteriology 71: 9-18 https://doi.org/10.1111/j.1365-2672.1991.tb04581.x
  4. Taub IA, Halliday JW, Sevilla MD. 1979. Chemical reactions in proteins irradiated at subfreezing temperatures. Adv Chem Ser 180: 109-140 https://doi.org/10.1021/ba-1979-0180.ch006
  5. World Health Organization. 1992. Review of the safety and nutritional adequacy of irradiated food. WHO/HPP/FOS/ 92.2
  6. Roberts T, Unneverhr L. 1994. New approaches to regulating food safety. Food Rev 17: 2-8
  7. World Health Organization. 1999. High-dose irradiation: Wholesomeness of food irradiated with dises above 10 kGy. Report of a Joint FAO/IAEA/WHO study group, WHO Technical Report Series 890. WHO, Geneva
  8. Yook HS, Lee YS, Lee JW, Oh SH, Kim JH, Kim DS, Byun MW. 2004. Textural and sensory characteristics of gamma irradiated porridges. J Korean Soc Food Sci Nutr 33: 427-432 https://doi.org/10.3746/jkfn.2004.33.2.427
  9. Holm NW, Berry RJ. 1970. Manual on radiation dosimetry. Marcel Dekker Inc., New York
  10. Maron DM, Ames BN. 1983. Revised methods for the Salmonella mutagenicity test. Mutat Res 113: 173-215 https://doi.org/10.1016/0165-1161(83)90010-9
  11. Koyama H, Utakoji T, Ono T. 1970. A new cell line derived from newborn Chinese hamster lung tissue. Gann 61: 161-167
  12. Hayashi M. 1991. The micronucleus test. Scientist Press, Inc., Tokyo, Japan
  13. Ames BN, McCann J, Yamasaki E. 1975. Methods for detecting carcinogens and mutagens with the Salmonella/ mammalian-microsome mutagenicity test. Mutat Res 31: 347-364 https://doi.org/10.1016/0165-1161(75)90046-1
  14. Wakata A, Sasaki MS. 1987. Measurement of micronuclei by cytokinesis-block method in cultured Chinese hamster cells: Comparison with types and rates of chromosome aberrations. Mutat Res 190: 51-57 https://doi.org/10.1016/0165-7992(87)90082-0
  15. Dean BJ, Danford N. 1984. Assays for the detection of chemically-induced chromosome damage in cultured mammalian cells. In Mutagenicity testing-a practical approach. Venitt S, Parry JM, eds. IRL Limited, Oxford, England. p 187-232
  16. Ishidate MJr, Sofuni T, Yoshikawa K. 1981. Chromosomal aberration tests in vitro as a primary screening tool for environmental mutagens and/or carcinogens. Gann Monograph on Cancer Res 27: 95-107
  17. Kang IJ, Kwak HJ, Lee BH, Kim KH, Byun MW, Yook HS. 1998. Genotoxicological and acute toxicological safeties of gamma irradiated beef. J Korean Soc Food Sci Nutr 30: 775-780
  18. Tanaka N. 1992. Induction of polyploids in bone marrow cells and micronuclei in reticulocytes in Chinese hamsters and rats fed with an irradiated wheat flour diet. In Final report of the Food Irradiation Research Committee for 1986-1991. Matsuyama A, ed. The Japan Isotopes Association, Tokyo, Japan. p 212-220
  19. Hayashi M, Yoshimura I, Sofuni T, Ishidate MJr. 1989. A procedure for data analysis of the rodent micronucleus test involving a historical control. Environ Mol Mutagen 13: 347-356 https://doi.org/10.1002/em.2850130412

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