Tribology and Lubricants

Korean Tribology Society (한국트라이볼로지학회)
권호 표지
DOI QR코드

DOI QR Code

Adhesion Strength Measurement of Rabbit Knee Chondrocyte

연골세포 부착력 평가

  • 이권용 (세종대 기계공학과 의공학연구소) ;
  • 박상국 (세종대 기계공학과 의공학연구소) ;
  • ;
  • 박종철 (연세대학교 의학공학교실)
  • Published : 2005.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

In order to prepare for the suitable surfaces of implants or medical devices, quantitative evaluation of adhesion between cells and biomaterials is essential. To better understand adhesion formation between cells and biomaterials, we used the cytodetachment technique which measures the adhesive force of a single cell through changing the, culture time and detachment speed. The results showed that the adhesive force could be affected by the culture time of cells on the surface of materials and the detachment speed. Moreover, there was a large discrepancy among the adhesion strength measured by similar techniques conducted on the different cells and substrates. It can be 'concluded that the variation of the force measurement technique can seriously alter the level of the force required to detach a cell on the surface of materials.

Keywords

References

  1. Edelman C. M., 'Cell adhesion molecules,' Science, pp. 291, 1983
  2. Sung K-LP, Kwan M. K., Maldonado F. and Akeson W. H., 'Adhision strength of human ligament fibroblasts'. Journal of Biomechanical Engineering, Vol. 116, pp. 237-242, 1994 https://doi.org/10.1115/1.2895725
  3. Garcia A. J., Ducheyne P. and Boettiger D., 'Quantification of cell adhesion using a spinning disc device and application to surface-reactive materials'. Biomaterials, Vol. 18, pp. 1091-1098, 1997 https://doi.org/10.1016/S0142-9612(97)00042-2
  4. Shin D. and Athanasiou K. A., 'Cytoindentation for obtaining cell biomechanical properties,' Journal of Orthopedic Research, Vol. 17, pp. 880-890, 1999 https://doi.org/10.1002/jor.1100170613
  5. Yamamoto A., Mishima S., Maruyama N. and Sumita M., 'Quantative evaluation of cell attachment to glass, polystyrene, and fibronectin- or collagen-coated polystyrene by measurement of cell adhesive shear force and cell detachment energy,' Journal of Biomedical Material Research, Vol. 50, pp. 114-124, 2000 https://doi.org/10.1002/(SICI)1097-4636(200005)50:2<114::AID-JBM4>3.0.CO;2-6
  6. Athanasio K. A., Thoma, B. S., Lanctot D. R., Shin D., Agrawal C. M. and Lebaron R. G., 'Development of the cytodetachment technique to quantify mechanical adhesiveness of the single cell,' Biomaterials, Vol. 20, pp. 2405-2415, 1999 https://doi.org/10.1016/S0142-9612(99)00168-4
  7. 김영지, 신정욱, 박기동, 이진우, 김정구, 지경수, 류수하, '세포와 구분자 표면간의 정량적 부착력 측정,' 생체재료학회지, 제6권, 제2호, pp. 65-71, 2002