Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Dark Hydrogen Production by a Green Microalga, Chlamydomonas reinhardtii UTEX 90

  • SIM SANG JUN (Department of Chemical Engineering, Sungkyunkwan University) ;
  • GONG GYEONG TAEK (School of Chemical Engineering, Seoul National University) ;
  • KIM MI SUN (Biomass Research Team, Korea Institute of Energy Research) ;
  • PARK TAl HYUN (School of Chemical Engineering, Seoul National University)
  • Published : 2005.12.01
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Abstract

The production of hydrogen by Chlamydomonas reinhardtii UTEX 90, a marine green alga, was performed under dark fermentation. The effects of initial nitrogen and phosphorus concentration on the cell growth and the production of hydrogen and organic substances were investigated. In the growth stage, the maximum dry cell weight (DCW) was 3 g/l when the initial ammonium concentration was 15 mM. In the dark fermentation, the maximum hydrogen production was $3.5\;{\mu}mol/\;mg$ DCW when the initial nitrogen concentration was 7.5 mM. The nitrogen concentration had a greater effect on organic compound and hydrogen production than the phosphorus concentration during the dark fermentation. An investigation of the duration of dark fermentation showed that, at least until three days, dark fermentation should be prolonged for maximum hydrogen production.

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