한국식품조리과학회지 (Korean journal of food and cookery science)

한국식품조리과학회 (Korean Society of Food and Cookery Science)
권호 표지

이산화탄소 유체를 이용한 녹차의 용매 추출물의 래디칼 소거 기능

Effect of Free Radical Scavenging from Green Tea Extraction using Carbon Dioxide Fluid

  • 강옥주 (경남대학교 식품영양학과)
  • Kang Ok-Ju (Department of Food Nutrition Science Kyungnam University)
  • 발행 : 2005.12.01
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초록

녹차 잎을 건조 분말하여 래디칼 소거기능을 추출하는 공정으로 순환 이산화탄소유체추출기를 활용할 때의 추출 최적조건을 구하였다. 건조한 녹차 잎의 분말사이즈는 80/100 mesh로 하고, 추출 압력과 온도를 각각 125 kgf 및 $25^{\circ}C$로 12시간 추출한 경우 가장 추출효율이 좋았다. 또한 용매를 열수추출과 에탄을 농도 를 달리한 추출 방법으로 나누어 래디칼 소거기능을 추출하였다. 그 결과, 에탄올 $95\%$농도에서 추출한 경우 가장 효율이 높았으며, 온도는 $100^{\circ}C$로 30분 추출이 최적 조건이었다. 각 추출 방법에 따른 추출 수율과 안정성을 비교한 결과, 이산화탄소유체를 이용한 녹차 래디칼 소거기능 추출 공정이 열수나 에탄올에 비하여 각각 2.8배 및 1.87배 수율이 높았으며, 안정성 실험에서도 활성의 소실이 거의 없었다.

Green tea was extracted with different solvents such as hot water, varying concentrations of ethanol and carbon dioxide fluid. The carbon dioxide fluid extraction of green tea was a very effective extraction method in terms of antioxidant yield and stability. At pressure of 125kgf, antioxidant extract of green tea produced a yield 1.87 and 2.8 times higher than hot water and $95\%$ ethanol extract, respectively. Antioxidant extract of carbon dioxide fluid was also very stable during 7-day storage at 35?.

키워드

참고문헌

  1. Blois M.S. 1958. Antioxidant determination by use of stable free radical, Nature 26: 1199-1203
  2. Choi HJ, Lee WS, Hwang SJ, Lee IJ, Shin DH, Kim HY, Kim KU. 2000. Changes in chemical compositions of green tea(Camellia sinensis L.) under the different extraction conditions. Korean J Life Sci 10: 202-209
  3. Choi HJ. 1977. Changes in chemical compositions of green tea (Camellia sinensis L.) under the different extraction conditions. MS Thesis, Kyungpook National Univ., Daegu
  4. Hai L, Sun H, Li Z. 1998. Simultaneous extraction of tea polyphenol and caffeine from green tea. Wei Sheng Yan Jiu 27: 195-196
  5. Hara Y, Ishigami T. 1989. Antibacterial activities of tea polyphenols against food borne pathogenic bacteria. Nippon Shokuhin Logyo gakkaishi 36: 996-999 https://doi.org/10.3136/nskkk1962.36.12_996
  6. Hibasami H, Achiwa Y, Fujikawa T, Komiya T. 1996. Induction of programmed cell death (apotosis) in human lymphoid leukemia cells by catechin compounds. Anticancer Res 16: 1942-1946
  7. J.I. Folch, I. Ascoll, M. Lees, J.A.Meath, F.N.Lebaron. 1951. Preparation of lipid extracts from brain tissue. J. Biol. Chem., 191: 833-841
  8. Kuhr S, Engelhardt U.H. 1991. Determination of flavanols, theogallin, gallic acid and caffeine in tea using HPLC, Z Lebensm unters forsch 192: 526-529 https://doi.org/10.1007/BF01202507
  9. Lee YJ, Ahn MS, Oh WT. 1998. A study on the catechins contents and antioxidative effect of various solvent extracts of green, oolong and black tea. J Fd Hyg Safety 13: 370-376
  10. Matsumoto M, Ishigaki F, Ishigaki A, Iwashina H, Hara Y. 1993. Reduction of blood glucose levels by tea catechin. Biosci Biotech Biochem 57: 525-527 https://doi.org/10.1271/bbb.57.525
  11. Muramatsu K, Fukuyo M, Hara Y. 1986. Effect of green tea catechins on plasma cholesterol level in cholesterol-fed rats. J Nutri Sci Vitaminol 32: 613-622 https://doi.org/10.3177/jnsv.32.613
  12. Row KH, Jin Y. 2005. Recovery of catchin compounds from Korean tea by solvent extraction. Bioresource technology in Press
  13. SAS Institute, Inc. 1985. SAS User's Guide; Statistics. 5th ed. Cary, NC, USA
  14. Sazuka M, Imazawa H, Shoji Y, Mita T, Hara Y, Isemura M. 1997. Inhibition of collagenases from mouse lung carcinoma cells by green tea catechins and black tea theaflavins. Biosci Biotech Biochem 61: 1504-1506 https://doi.org/10.1271/bbb.61.1504
  15. Yeo S, Ahn CY, Lee Y, Lee T, Park Y, Kim S. 1995. Antioxidative effect of tea extract from green tea, oolong tea and black tea. J Korean Soc Food Nutri 24(2), 299-304